Cell interactions and cytotoxic studies of cellulose nanofibers from Curauá natural fibers

Souza, Sivoney Ferreira de; Marcos, M.; Reis, Dennys; Lombello, Christiane Bertachini; Ferreira, M.; Sain, Mohini

doi:10.1016/j.carbpol.2018.08.056

Cited by 44 publications

(17 citation statements)

References 34 publications

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“…After 7 days of culture, we evidenced a qualitative difference in the morphology of cells cultured in C1, which appeared more elongated, compared to cells embedded inside crosslinked alginate, still characterized by a more spherical morphology, as confirmed by higher magnification images (Figure 8). This difference can be given by the 3D structure of TOUS-CNFs, which mimic the extracellular matrix (ECM) structure and subsequently promote cell spreading, as demonstrated for spread cells cultured on CNF films [66]. Thus, despite the debate on the safety of using nanocellulose fibers for biomedical applications [67], our data suggest the absence of indirect cytotoxic effects together with the possibility of embedding viable cells inside TOUS-CNFs/Ca 2+ hydrogels.…”

Section: Cytocompatibility Of 2266-tetramethylpiperidine 1-oxyl (Tmentioning

confidence: 71%

TEMPO-Nanocellulose/Ca2+ Hydrogels: Ibuprofen Drug Diffusion and In Vitro Cytocompatibility

et al. 2020

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Stable hydrogels with tunable rheological properties were prepared by adding Ca2+ ions to aqueous dispersions of 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO)-oxidized and ultra-sonicated cellulose nanofibers (TOUS-CNFs). The gelation occurred by interaction among polyvalent cations and the carboxylic units introduced on TOUS-CNFs during the oxidation process. Both dynamic viscosity values and pseudoplastic rheological behaviour increased by increasing the Ca2+ concentration, confirming the cross-linking action of the bivalent cation. The hydrogels were proved to be suitable controlled release systems by measuring the diffusion coefficient of a drug model (ibuprofen, IB) by high-resolution magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy. IB was used both as free molecule and as a 1:1 pre-formed complex with β-cyclodextrin (IB/β-CD), showing in this latter case a lower diffusion coefficient. Finally, the cytocompatibility of the TOUS-CNFs/Ca2+ hydrogels was demonstrated in vitro by indirect and direct tests conducted on a L929 murine fibroblast cell line, achieving a percentage number of viable cells after 7 days higher than 70%.

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Section: Cytocompatibility Of 2266-tetramethylpiperidine 1-oxyl (Tmentioning

confidence: 71%

TEMPO-Nanocellulose/Ca2+ Hydrogels: Ibuprofen Drug Diffusion and In Vitro Cytocompatibility

et al. 2020

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“…During the isolation of CNF, it is exposed to some physical, chemical, and even mechanical changes that may affect its toxicity to cells. Many studies confirm that there is no sign of toxicity in pure CNF [ 85 , 86 , 87 ], and other studies reported low or no significant toxicity [ 84 , 88 ], making it possible that either the preparation method or the source of CNF affect the cytotoxicity level. The long-term effect of most of the used materials is yet to be revealed, as well as the impact of them inside the body.…”

Section: Preparation Of Cnf Aerogel and Propertiesmentioning

confidence: 99%

A Review on Plant Cellulose Nanofibre-Based Aerogels for Biomedical Applications

et al. 2020

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Cellulose nanomaterials from plant fibre provide various potential applications (i.e., biomedical, automotive, packaging, etc.). The biomedical application of nanocellulose isolated from plant fibre, which is a carbohydrate-based source, is very viable in the 21st century. The essential characteristics of plant fibre-based nanocellulose, which include its molecular, tensile and mechanical properties, as well as its biodegradability potential, have been widely explored for functional materials in the preparation of aerogel. Plant cellulose nano fibre (CNF)-based aerogels are novel functional materials that have attracted remarkable interest. In recent years, CNF aerogel has been extensively used in the biomedical field due to its biocompatibility, renewability and biodegradability. The effective surface area of CNFs influences broad applications in biological and medical studies such as sustainable antibiotic delivery for wound healing, the preparation of scaffolds for tissue cultures, the development of drug delivery systems, biosensing and an antimicrobial film for wound healing. Many researchers have a growing interest in using CNF-based aerogels in the mentioned applications. The application of cellulose-based materials is widely reported in the literature. However, only a few studies discuss the potential of cellulose nanofibre aerogel in detail. The potential applications of CNF aerogel include composites, organic–inorganic hybrids, gels, foams, aerogels/xerogels, coatings and nano-paper, bioactive and wound dressing materials and bioconversion. The potential applications of CNF have rarely been a subject of extensive review. Thus, extensive studies to develop materials with cheaper and better properties, high prospects and effectiveness for many applications are the focus of the present work. The present review focuses on the evolution of aerogels via characterisation studies on the isolation of CNF-based aerogels. The study concludes with a description of the potential and challenges of developing sustainable materials for biomedical applications.

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“…[ 127 ]. On the medical side, many studies evaluated the biocompatibility and cytotoxicity of cellulose-based aerogels [ 128 , 129 , 130 , 131 ], and it was concluded that no cytotoxicity has been observed with cellulose-based aerogels, confirming that they can be safely incorporated with antibacterial substances as a promising material for dressing and healing of wounds.…”

Section: Biopolymer-based Aerogels For Antibacterial Deliverymentioning

confidence: 99%

A Review on Revolutionary Natural Biopolymer-Based Aerogels for Antibacterial Delivery

et al. 2020

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A biopolymer-based aerogel has been developed to become one of the most potentially utilized materials in different biomedical applications. The biopolymer-based aerogel has unique physical, chemical, and mechanical properties and these properties are used in tissue engineering, biosensing, diagnostic, medical implant and drug delivery applications. Biocompatible and non-toxic biopolymers such as chitosan, cellulose and alginates have been used to deliver antibiotics, plants extract, essential oils and metallic nanoparticles. Antibacterial aerogels have been used in superficial and chronic wound healing as dressing sheets. This review critically analyses the utilization of biopolymer-based aerogels in antibacterial delivery. The analysis shows the relationship between their properties and their applications in the wound healing process. Furthermore, highlights of the potentials, challenges and proposition of the application of biopolymer-based aerogels is explored.

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Cell interactions and cytotoxic studies of cellulose nanofibers from Curauá natural fibers

Cited by 44 publications

References 34 publications

TEMPO-Nanocellulose/Ca2+ Hydrogels: Ibuprofen Drug Diffusion and In Vitro Cytocompatibility

TEMPO-Nanocellulose/Ca2+ Hydrogels: Ibuprofen Drug Diffusion and In Vitro Cytocompatibility

A Review on Plant Cellulose Nanofibre-Based Aerogels for Biomedical Applications

A Review on Revolutionary Natural Biopolymer-Based Aerogels for Antibacterial Delivery

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