Cell interactions in the suppression of in vitro antibody responses.

Calkins, Catherine E.; Orbach-Arbouys, S.; Stutman, Osias; Gershon, Richard K.

doi:10.1084/jem.143.6.1421

Cited by 27 publications

(16 citation statements)

References 14 publications

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“…Durkin et al (23) demonstrated that low numbers of OVA-sensitized T memory cells added to higher numbers of lymph node cells from the same OVA-sensitized rats sometimes helped or potentiated and sometimes totally suppressed proliferative responses to either OVA or phytohemagglutinin. In the present studies, as was true of the earlier studies by others (20 -23), the mechanisms involved in the network(s) leading to help/potentiation or suppression of memory responses obviously are complex, involving other cells and mediators released by cells participating in the memory response and feedback loops (24,25).…”

Section: Discussionsupporting

confidence: 65%

Two Distinct T Cell Subsets, CD4+ and CD8+CD60+, and Their Cytokines Are Required for In Vitro Induction of Human Ragweed-Specific Memory IgE Responses

Smith‐Norowitz

Silverberg

Norowitz

et al. 2008

The Journal of Immunology

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CD8+CD60+ T cells (80–98% CD45RO+; 20% CD23+) are significantly increased in the blood of serum IgE+ ragweed-sensitized (RS) compared with serum IgE-nonatopic humans (p = 0.001). CD8+CD60+ T cells of the RS patients produced IL-2, IL-4, IL-10, IL-12, IFN-α. and IFN-γ, but not IL-6 or IL-13. When their PBMC were cultured with ragweed Ag (RA), peak IgE responses occurred on day 10; none was induced with non-cross-reacting or without Ag; nonatopic PBMC did not respond to any stimulant. When either CD4+ or CD8+CD60+ T cells were depleted from RS PBMC before culture with RA, no IgE responses were induced. If purified CD4+ T cells or low numbers of CD8+CD60+ T cells were added back to the depleted PBMC, IgE responses were restored. However, higher numbers of CD8+CD60+ T cells totally suppressed IgE responses. Total suppression also was obtained when RS PBMC were cultured with RA and either anti-IL-2, IL-4, IL-10, IL-12, IFN-γ (all concentrations), or IFN-α (low concentrations), but not anti-IL-6 or IL-13. Higher concentrations of anti-IFN-α potentiated IgE responses.

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Section: Discussionsupporting

confidence: 65%

Two Distinct T Cell Subsets, CD4+ and CD8+CD60+, and Their Cytokines Are Required for In Vitro Induction of Human Ragweed-Specific Memory IgE Responses

Smith‐Norowitz

Silverberg

Norowitz

et al. 2008

The Journal of Immunology

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“…SRBC and TNP-CRBC were used as antigens. The preparation of TNP-CRBC has been described elsewhere (20), as have the controls for the effect of increased cell numbers on these responses (11). The procedure of mitogen stimulation of proliferation was done as described previously (21).…”

Section: Methodsmentioning

confidence: 99%

“…Experimental Protocol. As reported previously (11), immune spleen cells from adult mice primed 8 days earlier with SRBC and pertussis were put into Mishell and Dutton-type cultures with normal spleen cells from adult (greater than 10 wk) or young (3-21 days) animals. Duplicate cultures were assayed for direct and indirect plaque-forming cells (PFC) on days 4 and 5 by using a slide modification (19) of the Jerne plaque assay.…”

Section: Methodsmentioning

confidence: 99%

“…In a previous report, we described the activity of specific suppressor cells among normal splenic lymphocytes cultured in the presence of specifically primed spleen cells (11). When these two cell populations (normal and sheep erythrocyte [SRBC] immune) were mixed and challenged with SRBC, both the primary response of the normal cells and the secondary response of the immune cells were suppressed.…”

mentioning

confidence: 99%

“…The suppression is specific in that the primary response to trinitrophenylated chicken erythrocytes (TNP-CRBC) is not suppressed in the same cultures that show suppressed SRBC responses. That the suppressor cells are mature thymus-derived lymphocytes (T cells) is suggested by their nonadherence to nylon wool (11), their presence in lymph node and cortisoneresistant thymus cell populations, their lower sensitivity to anti-Thy. 1 and C, and their relative absence from the normal thymus (14).…”

mentioning

confidence: 99%

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Changes in suppressor mechanisms during postnatal development in mice.

Calkins¹,

Stutman²

1978

The Journal of Experimental Medicine

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The activity of suppressor cells from spleens of mice of varying ages was assessed by their addition to cultures of normal or SRBC immune spleen cells together with a challenge of SRBC. 1-wk and adult spleen cells were highly suppressive of the secondary in vitro antibody response to SRBC. 3-wk spleen cells were less active in suppressing this response. The nature of the suppression and the character of the suppressor cells changed in this period. Whereas adult spleen cells demonstrated specificity, 1-wk cells nonspecifically suppressed all responses tested. Further, unlike adult suppressor cells (which are Thy.1.2 positive), 1-wk suppressor cells are insensitive to anti-Thy.1.2 treatment in this system. Both cells are nonadherent to glass beads and nylon wool and are undetectable in the normal thymus.

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Regulation of the immune response. I. Regulatory cell equilibrium in the “virgin” state

Kelsoe

Černý

1978

Eur J Immunol

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Spleen cells from pairs of inbred mice (age- and sex-matched) were challenged in vitro with a T-independent antigen. The specific plaque-forming cell (PFC) response in cultures containing cells from both donors was not intermediate to the responses of independently cultured donor spleen cells. Instead, a pattern of significant suppression (67% of pairs tested) or enhancement (22% of pairs tested) was observed. The suppression or enhancement was antigen-specific and did not represent a general phenomenon within the mixed cultures. A suppressive outcome in mixed cell cultures seems to be associated with cells from the donor with the higher individual PFC response. The converse is true for enhancement. The nonadditive nature of the PFC response in mixed cultures and the frequency of suppression to enhancement (3:1) imply that (a) specific, ongoing immunoregulation occurs even in the naive individual, and (b) this regulation is characterized by regular cycles of specific suppression and enhancement.

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Cell interactions in the suppression of in vitro antibody responses.

Abstract: Initiation of humoral immune responses requires a complex series of interac-

Cited by 27 publications

References 14 publications

Two Distinct T Cell Subsets, CD4+ and CD8+CD60+, and Their Cytokines Are Required for In Vitro Induction of Human Ragweed-Specific Memory IgE Responses

Two Distinct T Cell Subsets, CD4+ and CD8+CD60+, and Their Cytokines Are Required for In Vitro Induction of Human Ragweed-Specific Memory IgE Responses

Changes in suppressor mechanisms during postnatal development in mice.

Regulation of the immune response. I. Regulatory cell equilibrium in the “virgin” state

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