Microbial Pathogenesis

2020

DOI: 10.1016/j.micpath.2019.103860

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Cell-mediated immune response associated with Chlamydia pneumoniae infection in atherosclerotic patients

Loubna El Yazouli

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Fouad Seghrouchni

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et al.

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“…cell apoptosis was determined using an Epics XL. MCL flow cytometer (Beckman Coulter, Inc.) and analyzed using FlowJo version 10.6 software (FlowJo LLC) (21).…”

Section: Reverse Transcription-quantitative Pcr (Rt-qpcr)mentioning

confidence: 99%

HIF‑1α affects trophoblastic apoptosis involved in the onset of preeclampsia by regulating FOXO3a under hypoxic conditions

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et al. 2020

Mol Med Report

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Preeclampsia (Pe) is a pregnancy-specific syndrome that has severe implications on perinatal mortality and morbidity. excessive apoptosis of trophoblasts induced by hypoxia may be associated with the development of Pe, but the exact pathogenesis is unknown. Forkhead box o transcription factor 3a (FoXo3a) is activated under hypoxic conditions. Furthermore, hypoxia-inducible factor-1α (HiF-1α) is sensitive to variations in partial oxygen pressure. Thus, the aims of the present study were to investigate the expression levels of HiF-1α and FoXo3a in placental samples of early onset severe Pe, and their effect on trophoblastic apoptosis under hypoxic conditions. cobalt chloride was used to establish the hypoxic model. The present study examined the expression levels of HiF-1α and FoXo3a in the placental tissues and HTr8/SVneo cells under hypoxic conditions. it was found that HiF-1α and FoXo3a were highly expressed in placental tissues of patients with Pe and in HTr8/SVneo cells under hypoxic conditions. Furthermore, knockdown of FOXO3a using a specific small interfering rna (sirna) decreased apoptosis in HTr8/SVneo cells. Moreover, it was found that after knockdown of HiF-1α using sirna, FoXo3a expression and the apoptotic rate were reduced in HTr8/SVneo cells. Therefore, the present results indicated that the elevated expression of HiF-1α increased trophoblastic apoptosis by regulating FoXo3a, which may be involved in the pathogenesis of Pe.

“…cell apoptosis was determined using an Epics XL. MCL flow cytometer (Beckman Coulter, Inc.) and analyzed using FlowJo version 10.6 software (FlowJo LLC) (21).…”

Section: Reverse Transcription-quantitative Pcr (Rt-qpcr)mentioning

confidence: 99%

HIF‑1α affects trophoblastic apoptosis involved in the onset of preeclampsia by regulating FOXO3a under hypoxic conditions

¹

,

²

,

³

et al. 2020

Mol Med Report

View full text Add to dashboard Cite

Preeclampsia (Pe) is a pregnancy-specific syndrome that has severe implications on perinatal mortality and morbidity. excessive apoptosis of trophoblasts induced by hypoxia may be associated with the development of Pe, but the exact pathogenesis is unknown. Forkhead box o transcription factor 3a (FoXo3a) is activated under hypoxic conditions. Furthermore, hypoxia-inducible factor-1α (HiF-1α) is sensitive to variations in partial oxygen pressure. Thus, the aims of the present study were to investigate the expression levels of HiF-1α and FoXo3a in placental samples of early onset severe Pe, and their effect on trophoblastic apoptosis under hypoxic conditions. cobalt chloride was used to establish the hypoxic model. The present study examined the expression levels of HiF-1α and FoXo3a in the placental tissues and HTr8/SVneo cells under hypoxic conditions. it was found that HiF-1α and FoXo3a were highly expressed in placental tissues of patients with Pe and in HTr8/SVneo cells under hypoxic conditions. Furthermore, knockdown of FOXO3a using a specific small interfering rna (sirna) decreased apoptosis in HTr8/SVneo cells. Moreover, it was found that after knockdown of HiF-1α using sirna, FoXo3a expression and the apoptotic rate were reduced in HTr8/SVneo cells. Therefore, the present results indicated that the elevated expression of HiF-1α increased trophoblastic apoptosis by regulating FoXo3a, which may be involved in the pathogenesis of Pe.

“…In addition, adaptive immune response, a biological process in which antigen-specific T/B lymphocytes activate, proliferate, and differentiate into effector cells after receiving antigen stimulation, is involved in innate and adaptive immunity [ 42 ]. Previous study revealed that the expression of CD4 might be increased during inflammation and thrombosis, altering the immune cell-mediated response and leading to atherosclerosis [ 43 ]. In addition, Noble et al [ 44 ] found that CD4 were increased in the lamina propria of inflamed inflammatory bowel disease tissue.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis Exploring the Molecular Mechanisms of Hanchuan Zupa Granules in Alleviating Asthma in Rat

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et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Context. Hanchuan Zupa granule (HCZP), as a Chinese traditional medicine, is used to treat asthma. Objective. To investigate the molecular mechanisms of HCZP treatment of asthma. Materials and Methods. Thirty Sprague Dawley (SD) rats were divided into normal, asthma, and HCZP groups (n = 10). The asthma model was sensitized by 1 mg ovalbumin (OVA)/aluminum hydroxide Al(OH)3mixture and then challenged with 1% aerosolized OVA for four weeks. Rats in the HCZP group received 10.08 g/kg/d HCZP for four weeks during OVA challenge. Then, lung tissues of rats in each group were collected for RNA sequencing. Moreover, the expression level of some core genes was detected by using western blotting and immunohistochemistry. Results. Inflammatory cell infiltration and pathological damage of the lungs improved in the HCZP group. Compared with the asthma group (0.049 ± 0.002 mm2/mm; 0.036 ± 0.006 mm2/mm; and 0.014 ± 0.001 mm2/mm), total wall thickness (0.042 ± 0.001 mm2/mm), inner wall thickness (0.013 ± 0.001 mm2/mm), and smooth muscle layer thickness (0.012 ± 0.001 mm2/mm) significantly decreased in the HCZP group. Bioinformatics analysis showed that hub genes such as bradykinin receptor B2 (Bdkrb2) and CD4 molecule (Cd4) had different expression patterns between model and HCZP groups. Two transcription factors, forkhead box Q1 (Foxq1) and nuclear factor of activated T cells 2 (Nfatc2), served important regulatory roles in asthma. Compared with the model group, Bdkrb2 protein expression increased and Nfatc2 protein expression decreased in the HCZP group. Discussion and Conclusion. HCZP could alleviate asthma via regulating the expression of several hub genes, which might serve as therapeutic targets for asthma. However, the mechanism of these genes will be studied in the future.

“…The activation of cell-mediated immune responses is presumed to be significant for protective immunity against this infection. The cytokine response of monocytes/macrophages contributes to two situations; it may contribute to infection control and induction of immune reactive disorders (31).…”

Section: Discussionmentioning

confidence: 99%

Investigation of Possible Role of Chlamydia in Pseudoexfoliation Syndrome

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et al. 2021

Jundishapur J Microbiol

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Background: Pseudoexfoliation syndrome (PES) is a systemic disease characterized by the aggregation of fibrillar extracellular material in intraocular and extraocular tissues with unknown etiology. Clarifying the etiopathogenesis of PES would be important for public health. Objectives: We aimed to investigate the possible role of Chlamydia in the etiology of PES. Methods: This cross-sectional study was carried out in the ophthalmology clinic of a tertiary hospital. The study included two groups, including the patient group (PES patients with nuclear cataracts) and the control group (patients with nuclear cataracts). Patients with other ophthalmic problems and systemic diseases were excluded. Blood samples and conjunctival swabs taken from 49 patients and 42 controls were used in the study. Anti-Chlamydia trachomatis IgG and IgM, anti-C. pneumoniae IgG and IgM, Interleukin (IL)-6, and IL-20 were studied in the serum samples. The PCR study was performed with conjunctival swab samples and sequence analysis of PCR-positive samples was performed. Results: According to the results of the study, there was no statistically significant difference between the groups in terms of anti-C. trachmatis IgG, anti-C. trachmatis IgM, anti-C. pneumoniae IgM, IL-6, and PCR results. There was a statistically significant difference between patient and control groups in terms of anti-C. pneumoniae IgG and IL-20 levels. The DNA sequencing of all PCR products was found to be compatible with C. pneumoniae. Conclusions: It seems that C. pneumoniae might have an important role in the etiology and development of PES. However, further studies in larger groups are needed to clarify these results.

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