Cell-mediated immune response to epitopic MAP (multiple antigen peptide) construct of LcrV antigen of Yersinia pestis in murine model

LcrV Delivered via Type III Secretion System of Live Attenuated Yersinia pseudotuberculosis Enhances Immunogenicity against Pneumonic Plague

“…ϩ T-cell immune responses primed to LcrV appear to confer protection against Y. pestis in mice (48,49). Studies demonstrated that Chinese-origin rhesus macaques immunized with EV76 or mice immunized with the Y. pestis ⌬smpB-ssrA mutant primed a higher anti-F1 IgG titer but an almost undetectable titer with respect to LcrV antigen (38,50), results that are consistent with other studies of animals immunized with the EV76 or KWC vaccine (51)(52)(53)(54)(55)(56)(57).…”

Flagellin adjuvanted F1/V subunit plague vaccine induces T cell and functional antibody responses with unique gene signatures

“…33 Multiple antigen peptide constructs of V antigen increases lymphoproliferative responses in mice, with highest levels of Th1 and Th17 cytokines in culture supernatants. 34 IL-17 appears to mediate enhanced mucosal protection, and neutralization of vaccine-induced IL-17 decreases the survival of mice following challenge with Yp. 32 Our results similarly showed that Flagellin/F1/V vaccine significantly increased the number of proliferating and IL-10 or IL-4 producing CD4 T cells.…”

MAP of F1 and V antigens from Yersinia pestis astride innate and adaptive immune response

“…This study was extended in designing of MAP, incorporating all the relevant B and T cell epitopes of V and F1 antigen. All the individual epitopes contributed to overall mucosal and humoral immune response [23] and cell mediated immune response by VMAP [24]. Recently, F1 MAP showed mucosal, humoral and cellular protection in a mouse model [25].…”