Cell migration and adhesion of a human melanoma cell line is decreased by cold plasma treatment

Schmidt, Anke; Bekeschus, Sander; Woedtke, Thomas von; Hasse, Sybille

doi:10.1016/j.cpme.2015.05.003

Cited by 67 publications

(45 citation statements)

References 40 publications

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“…Several theories have been proposed, including a decrease of cell adhesion [21,22], interruption of the cell cycle [23][24][25], induction of apoptosis [26][27][28][29], and DNA fragmentation [30]. However, it is not yet clear whether CAP-generated reactive species are crucial for apoptosis and its associated DNA strand break or whether plasma-induced direct DNA damage provokes cell cycle checkpoint signaling that leads to apoptosis [31].…”

Section: Introductionmentioning

confidence: 99%

The Canady Helios Cold Plasma Scalpel Significantly Decreases Viability in Malignant Solid Tumor Cells in a Dose-Dependent Manner

Rowe¹,

Cheng²,

Ly³

et al. 2018

Plasma

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Abstract:To determine appropriate treatment doses of cold atmospheric plasma (CAP), the Canady Helios Cold Plasma Scalpel was tested across numerous cancer cell types including renal adenocarcinoma, colorectal carcinoma, pancreatic adenocarcinoma, ovarian adenocarcinoma, and esophageal adenocarcinoma. Various CAP doses were tested consisting of both high (3 L/min) and low (1 L/min) helium flow rates, several power settings, and a range of treatment times up to 5 min. The impact of cold plasma on the reduction of viability was consistently dose-dependent; however, the anti-cancer capability varied significantly between cell lines. While the lowest effective dose varied from cell line to cell line, in each case an 80-99% reduction in viability was achievable 48 h after CAP treatment. Therefore, it is critical to select the appropriate CAP dose necessary for treating a specific cancer cell type.

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Section: Introductionmentioning

confidence: 99%

The Canady Helios Cold Plasma Scalpel Significantly Decreases Viability in Malignant Solid Tumor Cells in a Dose-Dependent Manner

Rowe¹,

Cheng²,

Ly³

et al. 2018

Plasma

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“…This discovery was reported in vitro and in vivo for different non-thermal plasmas, where studies were conducted on a variety of cancer types 6 including melanoma 7 , cervical cancer 8 , hepatocellular carcinoma, breast carcinoma 9 , papilloma, colon cancer, glioblastoma, etc. 6 .…”

Section: Introductionmentioning

confidence: 99%

Plasma-induced selectivity in bone cancer cells death

Canal

Fontelo

Hamouda

et al. 2017

Free Radical Biology and Medicine

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“…An important effect of CAP treatment is the changes in membrane and cytoskeletal architecture. In this way, the CAP induces a considerable malfunction of the cell and can thus also lead to its inactivation [42]. These effects have already been extensively investigated on bacterial membranes [43][44][45][46], but the effect of CAP on the eukaryotic cytoplasmic membrane has not yet been described.…”

Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma Treatment of Chondrosarcoma Cells Affects Proliferation and Cell Membrane Permeability

Haralambiev

Nitsch

Jacoby

et al. 2020

IJMS

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Chondrosarcoma is the second most common malign bone tumor in adults. Surgical resection of the tumor is recommended because of its resistance to clinical treatment such as chemotherapy and radiation therapy. Thus, the prognosis for patients mainly depends on sufficient surgical resection. Due to this, research on alternative therapies is needed. Cold atmospheric plasma (CAP) is an ionized gas that contains various reactive species. Previous studies have shown an anti-oncogenic potential of CAP on different cancer cell types. The current study examined the effects of treatment with CAP on two chondrosarcoma cell lines (CAL-78, SW1353). Through proliferation assay, the cell growth after CAP-treatment was determined. A strong antiproliferative effect for both cell lines was detected. By fluorescein diacetate (FDA) assay and ATP release assay, alterations in the cell membrane and associated translocation of low molecular weight particles through the cytoplasmic membrane were observed. In supernatant, the non-membrane-permeable FDA and endogenously synthesized ATP detected suggest an increased membrane permeability after CAP treatment. Similar results were shown by the dextran-uptake assay. Furthermore, fluorescence microscopic G-/F-actin assay was performed. G- and F-actin were selectively dyed, and the ratio was measured. The presented results indicate CAP-induced changes in cell membrane function and possible alterations in actin-cytoskeleton, which may contribute to the antiproliferative effects of CAP.

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Cell migration and adhesion of a human melanoma cell line is decreased by cold plasma treatment

Cited by 67 publications

References 40 publications

The Canady Helios Cold Plasma Scalpel Significantly Decreases Viability in Malignant Solid Tumor Cells in a Dose-Dependent Manner

The Canady Helios Cold Plasma Scalpel Significantly Decreases Viability in Malignant Solid Tumor Cells in a Dose-Dependent Manner

Plasma-induced selectivity in bone cancer cells death

Cold Atmospheric Plasma Treatment of Chondrosarcoma Cells Affects Proliferation and Cell Membrane Permeability

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