Cell Origins and Diagnostic Accuracy of Interleukin 27 in Pleural Effusions

Abstract

The objective of the present study was to investigate the presence of interleukin (IL)-27 in pleural effusions and to evaluate the diagnostic significance of pleural IL-27. The concentrations of IL-27 were determined in pleural fluids and sera from 68 patients with tuberculous pleural effusion, 63 malignant pleural effusion, 22 infectious pleural effusion, and 21 transudative pleural effusion. Flow cytometry was used to identify which pleural cell types expressed IL-27. It was found that the concentrations of pleural IL-27 in tuberculous group were significantly higher than those in malignant, infectious, and transudative groups, respectively. Pleural CD4+ T cells, CD8+ T cells, NK cells, NKT cells, B cells, monocytes, macrophages, and mesothelial cells might be the cell sources for IL-27. IL-27 levels could be used for diagnostic purpose for tuberculous pleural effusion, with the cut off value of 1,007 ng/L, IL-27 had a sensitivity of 92.7% and specificity of 99.1% for differential diagnosing tuberculous pleural effusion from non-tuberculous pleural effusions. Therefore, compared to non-tuberculous pleural effusions, IL-27 appeared to be increased in tuberculous pleural effusion. IL-27 in pleural fluid is a sensitive and specific biomarker for the differential diagnosing tuberculous pleural effusion from pleural effusions with the other causes.

Recruitment of IL-27-Producing CD4+ T Cells and Effect of IL-27 on Pleural Mesothelial Cells in Tuberculous Pleurisy

“…As expected, we observed that addition of IL-27Ra-Fc chimera into the TPE supernatant exerted a potent inhibitory activity for PMC wound healing, compatible with findings that high concentrations of IL-27 were detected in TPE [7]; in contrast, addition of anti-IFN-c mAb significantly improved PMC wound healing, compatible with findings that high concentrations of IFN-c were detected in TPE [10] (Fig. 4).…”

“…T cells, NK cells, NKT cells, B cells, monocytes, macrophages, as well as pleural mesothelial cells (PMCs) are cell sources of pleural IL-27 [7]. More recently, our data further showed that the numbers of IL-27-producing CD4 ?…”

Increased soluble and membrane-bound PD-L1 contributes to immune regulation and disease progression in patients with tuberculous pleural effusion

“…Patients were diagnosed with TPE on the basis of: i) Presence of acid fast bacilli in a pleural fluid specimen, growth of M. tb from pleural fluid, or demonstration of granulomatous pleurisy on closed pleural biopsy specimen in the absence of any evidence of other granulomatous diseases; ii) an exudative lymphocytic effusion with an adenosine deaminase level of >40 U/l, a positive purified protein derivative skin test result and the exclusion of any other potential causes of pleurisy; iii) or a good response to TB treatment (18). Exudative effusion was classified according the criteria outlined by Light et al (19).…”

Diagnostic accuracy of interleukin 27 for tuberculous pleural effusion: two prospective studies and one meta-analysis

“…A few years ago, we reported for the first time that interleukin 27 (IL-27)—a cytokine of the IL-12 cytokine family produced by antigen-presenting cells under the stimulation of pathogen-associated molecular patterns binding to their toll-like receptors—can distinguish between TPE and non-TPEs, with a sensitivity of 92.7% and a specificity of 99.1% 6. In another study we indicated that the diagnostic efficiency of IL-27 is better than that of ADA 7.…”