Pathological processes associated with disturbed keratinization and risks of cancer tend to become more common and affect younger people. However, the currently available diagnostic methods are not always reliable, which explains the need to further study this. The aim of the study was to improve the efficiency of early diagnosis of keratoses of the oral cavity under autofluorescence spectroscopy incorporated into the proposed screening algorithm. The examination methods involved clinical, luminescent, analytical, statistical evaluation. The study allowed obtaining optical images through autofluorescence stomatoscopy in patients with keratinization disorders, identifying their color range within affected and healthy tissues, as well as confirming their reliability employing the Color Spatioplotter ver 2.46 software. The tested autofluorescent stomatoscopy method featured sufficient sensitivity (98%) against relative specificity (75%), with prediction of a positive outcome (100%).