Cell proliferation and tumour suppressor gene expression in iodine unstained area surrounding oral squamous cell carcinoma

Yokoo, Keiko; Noma, Hiroyasu; Inoue, Takashi; Hashimoto, Sadamitsu; Shimono, Masaki

doi:10.1054/ijom.2002.0457

Cited by 23 publications

(28 citation statements)

References 26 publications

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“…Yajima et al reported that telomerase activity was significantly different between iodine-stained areas of normal epithelium and -unstained areas of epithelial dysplasia (17). In another study, p53 expression in iodine-unstained areas was markedly higher than in iodine-stained areas (14). Our findings along with those suggest that the Lugol-unstained area is consistent with premalignant and malignant lesions in the oral mucosa, and should be completely removed if it surrounds dysplasia or a squamous cell carcinoma.…”

Section: Discussionsupporting

confidence: 75%

“…Malignant epithelium has a limited amount of glycogen granules as compared with normal squamous epithelium (3). It has been reported that intermediate filaments, precursors of keratosis, in epithelial cells are increased in the cytoplasm according to the degree of epithelial dysplasia, while glycogen granules in moderate and severe dysplasia are significantly decreased compared with those in the normal epithelium (14). In the present study, all border areas stained by Lugol solution were normal or mild dysplastic epithelium corresponded with PAS-positive, which indicates parakeratotic epithelium containing a large amount of glycogen.…”

Section: Discussionmentioning

confidence: 99%

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Histopathological evaluation including cytokeratin 13 and Ki-67 in the border between Lugol-stained and -unstained areas

Ohta

Ogawa²,

Ono³

et al. 2010

Oncol Rep

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Abstract. Lugol's iodine staining (Lugol-staining) has been widely used to detect malignant changes in the cervix uteri and esophagus. However, pathological and histochemical changes in the border between Lugol-stained and -unstained areas in oral epithelial dysplastia and malignant lesions are not well understood. We examined the histological appearance of 20 cases of epithelial dysplasia surrounding squamous cell carcinoma using HE and PAS staining in the borders between Lugol-stained and -unstained areas. Subsequently, differences in the expression of cytokeratin 13 (CK13), an epithelium differentiation marker, and Ki-67, a cell proliferative marker, in those areas were investigated by immunohistochemistry. Lugol-stained areas of all cases showed mild dysplasia or normal epithelium appearance, while Lugol-unstained areas in most cases were diagnosed as moderate/severe dysplasia and carcinoma in situ. PAS reactions were limited or not found in the Lugol-unstained areas as compared to intense positivity in Lugol-stained areas. CK13 and Ki-67 protein expression was significantly different between Lugol-stained and -unstained areas. It was confirmed that epithelia showing precancerous or cancerous features were detected as Lugol-unstained boundary areas. A reduction in glycogen production caused by alterations of cell differentiation and proliferation associated with malignant changes may result in a lack of Lugol-staining.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Histopathological evaluation including cytokeratin 13 and Ki-67 in the border between Lugol-stained and -unstained areas

Ohta

Ogawa²,

Ono³

et al. 2010

Oncol Rep

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“…Toluidine blue by its property of retaining in the increased DNA and RNA cellular activity areas, aids in delineating the suspicious areas [2,3].…”

Section: Introductionmentioning

confidence: 99%

Utility of Toluidine Blue Test in Accessing and Detecting Intra-Oral Malignancies

Singh

Shukla²

2014

Indian J Otolaryngol Head Neck Surg

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In vivo staining reveals cytological details that might otherwise not be apparent. The aim of the study was to test the utility of toluidine blue test in detecting various types of malignant and premalignant lesions in early stage. Fifty patients with lesion in oral cavity having suspicion of malignancy clinically were selected. After subjecting the patients to clinical examination, the suspicious lesions were stained with 1 % toluidine blue. The biopsy site was selected on the basis of clinical appearance and dye retention and in the sites where no retention of the stain occurred, clinical judgment directed the biopsy site. The sensitivity of toluidine blue in detecting premalignant or malignant lesions was found to be 97.8 % and the over all specificity was found to be 100 %. The positive predictive value, negative predictive value and diagnostic accuracy was reported to be 100, 80 and 90 % respectively. Toluidine Blue staining is highly a reliable source for the detection of insitu and invasive carcinomas. Staining with this stains is an adjunct to clinical judgment, assist in the choice of biopsy site, follow up of premalignant lesions and marginal demarcation of the malignant lesions enabling an intervention method to be adopted earlier for the disease, which carries a high rate of morbidity and mortality.

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“…Yokoo et al 9 investigated the relationship between epithelial dysplasia unstained with iodine and the expression of proliferating cell nuclear antigen and/or the tumor suppressor gene (p53). They found that mild dysplastic epithelia stained with iodine may be in the category of normal epithelia, whereas both moderate and severe dysplasia unstained with iodine may be suggestive of malignant lesions.…”

Section: Discussionmentioning

confidence: 99%

“…Vital staining with iodine solution has been employed intra-operatively with success in determining the extent and precise border of the cancerous/dysplastic epithelium, ensuring complete resection of the tumor at the mucosal surface. [7][8][9][10][11] The use of vital staining as an adjunct to determining resection margins for oral dysplastic/cancerous lesions may, theoretically, improve the margin control of oral malignant lesions. Therefore, the purpose of this retrospective study was to assess the efficacy of vital staining with iodine solution to control local recurrence in superficially spread possibly malignant or malignant lesion of oral mucosa.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of vital staining with iodine solution in reducing local recurrence after resection of dysplastic or malignant oral mucosa

Kitamura

Kamata

et al. 2012

British Journal of Oral and Maxillofacial Surgery

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Abstract.The purpose of this retrospective study was to assess the efficacy of vital staining with iodine solution in reducing local recurrence in the resection of dysplastic or cancerous oral mucosa.The conventional group (a historical control group) underwent surgical resection of dysplastic or cancerous mucosa solely by direct inspection/palpation. The vital staining group did with the aid of vital staining with iodine solution.Seven of 25 patients (28.0%) in the conventional group experienced recurrence of dysplastic or cancerous oral mucosa around the primary site, while no patient reported recurrence in the vital staining group (Fisher's exact probability test, p<0.01).Kaplan-Meier assessment showed that the 5-year primary-control rate was 100% in the vital staining group and 75% in the conventional group.Although this retrospective study has some limitations, the results suggested the possible value of the vital staining with iodine solution in reducing the incidence of recurrence of dysplastic or cancerous epithelium at the same site. Further well-controlled study is mandatory.

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Cell proliferation and tumour suppressor gene expression in iodine unstained area surrounding oral squamous cell carcinoma

Cited by 23 publications

References 26 publications

Histopathological evaluation including cytokeratin 13 and Ki-67 in the border between Lugol-stained and -unstained areas

Histopathological evaluation including cytokeratin 13 and Ki-67 in the border between Lugol-stained and -unstained areas

Utility of Toluidine Blue Test in Accessing and Detecting Intra-Oral Malignancies

Effectiveness of vital staining with iodine solution in reducing local recurrence after resection of dysplastic or malignant oral mucosa

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