Cell proliferation markers in the transplanted canine transmissible venereal tumor

Santos, F.G.A.; Moro, Luciana; Cassali, Geovanni Dantas; Campos, Paula Peixoto; Silva, S.S.; Vasconcelos, A.C.

doi:10.1590/s0102-09352011000600010

Cited by 7 publications

(5 citation statements)

References 23 publications

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“…The number and area of NORs represent parameters that reflect the level of proliferative activity of cells (Santos et al 2011). Santos et al (2011, in a study on canine transmissible venereal tumor (TVT), demonstrated that the greater the number of small NORs, the greater the proliferative activity of cells, with tumors in the growth phase but this was not observed in the present study.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of cell proliferation and endometrial thickness of bitches in different periods of diestrus

Gossler

Santos

Azevedo

et al. 2017

An. Acad. Bras. Ciênc.

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This study aimed to determine the histological features of the endometrium of bitches, as well as the cell proliferation at specific moments of diestrus, 10, 20, 30, 40, 50 and 60 days post ovulation, correlating the endometrial thickness with the uterine cell proliferation and the metabolic state (weight, blood glucose and plasma cholesterol) of the animals. Therefore, the right and left uterine horns of 26 clinically healthy bitches submitted to ovariohysterectomy were histologically analyzed 10, 20, 30, 40, 50 and 60 days post ovulation. The hematoxylin-eosin and AgNOR staining techniques were performed. All parameters were evaluated by ANOVA and post-hoc Tukey test (p<0.05). The correlation between endometrial thickness and uterine cell proliferation, weight, blood glucose and plasma cholesterol of animals was observed using the Pearson method (p<0.05). In the present study, it is concluded that endometrial thickness does not differ at any of the moments analyzed in diestrus. The endometrial thickness is not influenced by hormones, weight, blood glucose or serum cholesterol of bitches in this phase of the estrous cycle. However, there is greater cell proliferation in the endometrium at day 40 compared to day 60 post ovulation under the influence of the endocrine profile.

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Section: Resultsmentioning

confidence: 99%

Evaluation of cell proliferation and endometrial thickness of bitches in different periods of diestrus

Gossler

Santos

Azevedo

et al. 2017

An. Acad. Bras. Ciênc.

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“…Ki-67 PI is related to tumor progression, metastatic ability, and prognostic value (30, 31, 36). It was higher in the pre-treated samples than the post-treated samples.…”

Section: Discussionmentioning

confidence: 99%

“…All slides were immunostained with polymer-based, non-avidin-biotin EnVision detection system TM (Dako, Denmark), and 3, 3′-diaminobenzidine tetrahydrochloride (DAB) substrate (Dako, Denmark) as a visualization system. The Ki-67 PI was defined as the percentage of nuclear immunolabeling positive cells (at least 1,000 cells were counted) among the total number of tumor cells counted in at least 10 HPF (36).…”

Section: Methodsmentioning

confidence: 99%

Conventional-Vincristine Sulfate vs. Modified Protocol of Vincristine Sulfate and L-Asparaginase in Canine Transmissible Venereal Tumor

et al. 2019

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Background: Vincristine (VCR) is a mono-chemotherapy for canine transmissible venereal tumor (CTVT). L-asparaginase (LAP) is usually used in combination with other drugs. Previously, LAP-VCR protocol was applied for the CTVT-VCR-resistant cases. However, there were a few reports about using this protocol since the first visit.Aims: To firstly investigate the effectiveness of combining chemotherapy (Vincristine and L-asparaginase, VCR-LAP) in normal CTVT case. Secondly, to compare this protocol with the conventional (Vincristine, VCR) protocol before and during treatment in 24 CTVT dogs.Materials and Methods: Clinical signs, tumor relative volume, and histopathological change [amount of CTVT cells, tumor-infiltrating lymphocytes (TILs), TILs/CTVT ratio, collagen area, and Ki-67 proliferative index (PI)] were the treatment evaluation parameters. Moreover, transcriptome analysis of apoptotic (Bcl-2, Bax), drug-resistant genes (ABCB1, ABCG2), and BCL-2 and BAX expression were also included.Results: Both protocols gave the decreased tumor volume, increased TILs/CTVT ratios and collagen area in the mass. Interestingly, the combination protocol decreased treatment time. There were two resistant cases after treatment with VCR. The expression of Bcl-2 and Bax were decreased, and this may indicate the better response after treatment. Moreover, both drug resistant genes did not increase after treatment.Conclusion: The main finding of this study is that the combination protocol did not only decrease treatment duration time but also gave the effectiveness of treatment outcomes in CTVT cases. Therefore, the application of the new protocol could be used by the field practitioners.

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“…The number of AgNOR is related to the cell cycle and proliferative activity of the cell 33 . The quantification of AgNOR area is related to the tumor growth rate, cell proliferative activity and consequently tumor progression 34 , 35 . Thus, we used AgNOR quantification to evaluate the antitumor effect of EEOb in vivo .…”

Section: Resultsmentioning

confidence: 99%

Ocimum basilicum L. (basil) presents pro-apoptotic activity in an Ehrlich’s experimental tumor murine model

Sant’Ana,

Lima,

Lopes

et al. 2024

Acta Cir. Bras.

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Purpose: This study aimed to evaluate the therapeutic effect of an ethanol extract of Ocimum basilicum L. (EEOb) aerial parts against Ehrlich’s experimental tumor (EET) in mice. Methods: Swiss mice were divided into two groups (control and treated; n = 6). On day 21, all mice were inoculated subcutaneously with 2 × 10 6 (0.05 mL) EET cells in the left paw for solid tumor development. This study lasted 28 days. Treatment began 24 hours after inoculation with EET. Measurements of dorsoplantar thickness were used to assess tumor growth. The paw pad was collected for histopathological analysis and stained using the argyrophilic nucleolar organizing regions (AgNOR) technique and immunohistochemistry for proliferating cell nuclear antigen, Bcl-2 and Bax. Results: The treatment of animals with EEOb at 100 mg/kg intraperitoneally was able to reduce the growth (Control = 3.7 ± 0.1 mm vs. EEOb = 5.7 ± 0.2 mm) and the number of AgNORs of solid Ehrlich tumor. The antitumor effect of EEOb was associated with the induction of apoptosis of tumoral cell, as suggested by the reduction of the content of Bcl-2 induced by extract. Conclusions: The study demonstrated that daily administration of EEOb is able to reduce the growth of EET by induce apoptosis of tumoral cells.

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Cell proliferation markers in the transplanted canine transmissible venereal tumor

Cited by 7 publications

References 23 publications

Evaluation of cell proliferation and endometrial thickness of bitches in different periods of diestrus

Evaluation of cell proliferation and endometrial thickness of bitches in different periods of diestrus

Conventional-Vincristine Sulfate vs. Modified Protocol of Vincristine Sulfate and L-Asparaginase in Canine Transmissible Venereal Tumor

Ocimum basilicum L. (basil) presents pro-apoptotic activity in an Ehrlich’s experimental tumor murine model

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