Cell proliferation studies in the intestinal epithelium of the rat

Cairnie, A. B.; Bentley, R. E.

doi:10.1016/0014-4827(67)90079-1

Cited by 141 publications

(93 citation statements)

References 15 publications

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“…In each animal the data were projected on to a standard crypt the height of which was the mean crypt height of the animal concerned. The previously described modification (Wright, Morley and Appleton, 1972) of the method of Cairnie and Bentley (1967) to compensate for variation in crypt height was used to produce labelling and mitotic-index distribution diagrams. The circumference of crypts was measured by counting the number of cells appearing in transverse sections of crypts containing metaphases: "crypt column count".…”

Section: Methodsmentioning

confidence: 99%

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Kinetics of changes in the crypts of the jejunal mucosa of dimethylhydrazine-treated rats

Sunter¹,

Appleton²,

Wright³

et al. 1978

Br J Cancer

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Summary.-When symmetrical 1,2 dimethylhydrazine was administered to rats by weekly s.c. injection, 37% of the animals had developed small intestinal carcinomas after 21-27 weeks. These lesions were largely localized to duodenum and upper jejunum. At the same time there was a diffuse crypt hyperplasia in the jejunum which affected all the treated animals, not just those with neoplasms. This marked hyperplasia was preceded by a modest sustained crypt elongation which was seen soon after DMH injections began.In these hyperplastic jejunal crypts the absolute size of the proliferative compartment was increased, but the growth fraction calculated from labelling studies appeared to fall, probably by reduction in relative size of the proliferating population within the proliferative compartment.No convincing alteration in actual cell-cycle time was observed in the abnormal crypts. There was a slight (25%) increase in cell-production rate in the abnormal crypts.

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Section: Methodsmentioning

confidence: 99%

“…Cairnie and Bentley (1967) described crypt hyperplasia as a normal physiological event during lactation. They found an increase in column count also, and a suggestion of increased migration rates; growth fraction remained uinchanged.…”

Section: -16mentioning

confidence: 99%

Kinetics of changes in the crypts of the jejunal mucosa of dimethylhydrazine-treated rats

Sunter¹,

Appleton²,

Wright³

et al. 1978

Br J Cancer

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“…The increased higher accumulation of villus tip supported the key role of mucin in the protection of gut epithelium against luminal threats. Furthermore, the decreased stain in the crypt tip compared with midsection and base may relate to the process of proliferation and maturation of goblet cells, which are present mainly in crypts, but can also occur along the entire length of the villus of the chicken (Cairnie et al, 1965;Uni et al, 1998).…”

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confidence: 99%

Modulation of intestinal mucin composition and mucosal morphology by dietary phytogenic inclusion level in broilers

Tsirtsikos

Fegeros

Kominakis

et al. 2012

Animal

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The effect of a dietary phytogenic feed additive (PFA) inclusion level in mucin monosaccharide composition, mucosal morphometry and mucus histochemistry along the broiler intestinal tract was studied. Cobb male broilers (n 5 525) were allocated into five experimental treatments that, depending on the type of addition in the basal diet (BD), were labeled as follows: C (BD based on maize-soybean meal with no other additions), E1 (80 mg PFA/kg BD), E2 (125 mg PFA/kg BD), E3 (250 mg PFA/kg of BD) and A (2.5 mg avilamycin/kg BD). Samples from duodenum, ileum and cecum of 14-and 42-day-old broilers were collected and analyzed. In 14-day-old broilers, treatments E2 and E3 had higher (P , 0.01) duodenal mannose than treatments C, E1 and A. Ileal mannose was lower (P , 0.05) in treatment C compared with PFA treatments, and ileal galactose (Gal) was higher (P , 0.01) in treatments E2 and E3 compared with C and A. Polynomial contrast analysis with respect to PFA inclusion level showed that in 14-day-old broilers there was a linear increase (P 5 0.001) in duodenal mannose and a quadratic effect (P 5 0.038) in duodenal N-acetyl-galactosamine with increasing PFA level. Ileal Gal and mannose increased linearly (P 5 0.002 and P 5 0.012, respectively) with PFA inclusion level. There were no significant differences between treatments in mucin monosaccharide molar ratios of 42-day-old broilers. However, increasing PFA inclusion level resulted in a linear decrease of ileal fucose (P 5 0.021) and cecal N-acetylgalactosamine (P 5 0.036). Experimental treatments did not differ (P . 0.05) regarding duodenal villus height (Vh), crypt depth (Cd) and Vh/Cd ratio, irrespective of broiler age and the intestinal segment examined. However, increasing dietary PFA inclusion level showed a pattern of linear increase of duodenal Vh/Cd ratio in 14-day-old broilers and ileal Vh in 42-day-old broilers (P 5 0.039 and P 5 0.039, respectively). Alcian Blue-Periodic AcidSchiff (pH 2.5) staining of neutral and acidic mucins showed that the staining intensity of mucus layer in villi was fragment (i.e. tip, midsection and base) dependent, whereas in crypts it was dependent both on intestinal segment (i.e. duodenum, ileum and cecum) and fragment. Finally, mucus layer thickness did not differ (P . 0.05) between treatments, yet a pattern of linear increase (P , 0.05) with PFA inclusion level was observed in the duodenum of 42-day-old broilers. In conclusion, the dietary inclusion level of PFA modulated broiler intestinal mucin composition and morphology. Further studies are required to elucidate the physiological implications of such changes in host-microflora interactions.Keywords: phytogenic feed additive, essential oils, broiler, mucin, intestinal morphology ImplicationsThis study has shown that the inclusion level of a phytogenic feed additive in broiler diets has resulted in changes in the gut mucin monosaccharide composition and the intestinal mucosal architecture morphology. Given the fact that animal performance and disease prevention are prerequisite...

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“…The criterion for a labelled nucleus was 5 or more autoradiographic grains located directly over it. Using a modification (Wright et al, 1972) of the method of Cairnie & Bentley (1967) diagrams showing the mean labelling and mitotic indices at each cell position in a crypt of mean length were produced for each sample.…”

Section: Methodsmentioning

confidence: 99%

Kinetics of the non-neoplastic mucosa of the large bowel of dimethylhydrazine-treated rats

Sunter¹,

Watson²,

Appleton³

1981

Br J Cancer

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Summary.-Administration of 1,2 dimethylhydrazine (DMH) to rats by weekly s.c. injections causes the development of multiple epithelial tumours of the large bowel. These appear to arise as localized dysplastic abnormalities in hitherto apparently morphologically normal crypts. This study was undertaken in order to examine cell proliferation in such apparently normal crypts of DMH-treated animals.A number of proliferative abnormalities are evident, including changes in the size of the crypts, changes in the disposition of proliferating cells within them and reduced cell-cycle times. The nature and the extent of the abnormalities vary from site to site along the length of the bowel, and reflect the vulnerability of the different segments of the bowel, not only to the carcinogenic effects of DMH, but also to short-term toxicity.

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Cell proliferation studies in the intestinal epithelium of the rat

Cited by 141 publications

References 15 publications

Kinetics of changes in the crypts of the jejunal mucosa of dimethylhydrazine-treated rats

Kinetics of changes in the crypts of the jejunal mucosa of dimethylhydrazine-treated rats

Modulation of intestinal mucin composition and mucosal morphology by dietary phytogenic inclusion level in broilers

Kinetics of the non-neoplastic mucosa of the large bowel of dimethylhydrazine-treated rats

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