Cell-sheet technology combined with a thienoindazole derivative small compound TD-198946 for cartilage regeneration

Yano, Fumiko; Hojo, Hironori; Ohba, Shinsuke; Saito, Taku; Honnami, Muneki; Mochizuki, Manabu; Takato, Tsuyoshi; Kawaguchi, Hiroshi; Chung, Ung‐il

doi:10.1016/j.biomaterials.2013.04.008

Cited by 25 publications

(18 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to these anabolic effects with chondrocytes, TD‐198946 acts as a chondrogenic inducer for mouse mesodermal cell line (Yano et al, ), mouse‐induced pluripotent cells (Saito et al, ), and human bone marrow‐derived mesenchymal stem cells in vitro (Yano et al, ). Therefore, TD‐198946 may be a potential reagent to induce chondrocyte differentiation and create cartilaginous tissue from hSSCs with an optimal protocol and with appropriate doses of the compound.…”

Section: Introductionmentioning

confidence: 99%

Effect of the small compound TD ‐198946 on glycosaminoglycan synthesis and transforming growth factor β3‐associated chondrogenesis of human synovium‐derived stem cells in vitro

Chijimatsu

Yano

Saito

et al. 2019

J Tissue Eng Regen Med

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of the small compound TD ‐198946 on glycosaminoglycan synthesis and transforming growth factor β3‐associated chondrogenesis of human synovium‐derived stem cells in vitro

Chijimatsu

Yano

Saito

et al. 2019

J Tissue Eng Regen Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…Our previous studies revealed that TD-198946 enhances GAG synthesis in mouse chondrocytes, human bone marrow stromal cells, and human synovium-derived stem cells (hSSCs) 10 , 17 , 18 . Moreover, our previous study identified a unique effect of TD-198946 on hyaluronan secretion by hSSCs 17 .…”

Section: Discussionmentioning

confidence: 99%

The small compound, TD-198946, protects against intervertebral degeneration by enhancing glycosaminoglycan synthesis in nucleus pulposus cells

Kushioka

Kaito

Chijimatsu

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Degeneration of the nucleus pulposus (NP) might serve as a trigger for intervertebral disc degeneration (IDD). A recent drug screening study revealed that the thienoindazole derivative, TD-198946, is a novel drug for the treatment of osteoarthritis. Because of the environmental and functional similarities between articular cartilage and intervertebral disc, TD-198946 is expected to prevent IDD. Herein, we sought to evaluate the effects of TD-198946 on IDD. TD-198946 enhanced glycosaminoglycan (GAG) production and the related genes in mouse NP cells and human NP cells (hNPCs). Further, Kyoto Encyclopedia of Genes and Genomes pathway analysis using the mRNA sequence of hNPCs suggested that the mechanism of action of TD-198946 primarily occurred via the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway. The Akt inhibitor suppressed the enhancement of GAG production induced by TD-198946. The effects of TD-198946 on IDD at two different time points (immediate treatment model, immediately after the puncture; latent treatment model, 2 weeks after the puncture) were investigated using a mouse tail-disc puncture model. At both time points, TD-198946 prevented a loss in disc height. Histological analysis also demonstrated the preservation of the NP structures. TD-198946 exhibited therapeutic effects on IDD by enhancing GAG production via PI3K/Akt signaling.

show abstract

“…After the cells were cultured for several days or weeks to form cell sheets, the temperature-responsive culture dishes were exposed to 20 °C or room temperature for a period of time, and polyvinylidene fluoride (PVDF) membranes or forceps were used to harvest the cell sheets. 20,21,43,44…”

Section: Preparation Of Cell Sheetsmentioning

confidence: 99%

Recent advances in cell sheet technology for bone and cartilage regeneration: from preparation to application

Zhang

Wang

et al. 2019

Int J Oral Sci

View full text Add to dashboard Cite

Bone defects caused by trauma, tumour resection, infection and congenital deformities, together with articular cartilage defects and cartilage-subchondral bone complex defects caused by trauma and degenerative diseases, remain great challenges for clinicians. Novel strategies utilising cell sheet technology to enhance bone and cartilage regeneration are being developed. The cell sheet technology has shown great clinical potential in regenerative medicine due to its effective preservation of cell-cell connections and extracellular matrix and its scaffold-free nature. This review will first introduce several widely used cell sheet preparation systems, including traditional approaches and recent improvements, as well as their advantages and shortcomings. Recent advances in utilising cell sheet technology to regenerate bone or cartilage defects and bone-cartilage complex defects will be reviewed. The key challenges and future research directions for the application of cell sheet technology in bone and cartilage regeneration will also be discussed.

show abstract

Cell-sheet technology combined with a thienoindazole derivative small compound TD-198946 for cartilage regeneration

Cited by 25 publications

References 33 publications

Effect of the small compound TD ‐198946 on glycosaminoglycan synthesis and transforming growth factor β3‐associated chondrogenesis of human synovium‐derived stem cells in vitro

Effect of the small compound TD ‐198946 on glycosaminoglycan synthesis and transforming growth factor β3‐associated chondrogenesis of human synovium‐derived stem cells in vitro

The small compound, TD-198946, protects against intervertebral degeneration by enhancing glycosaminoglycan synthesis in nucleus pulposus cells

Recent advances in cell sheet technology for bone and cartilage regeneration: from preparation to application

Contact Info

Product

Resources

About