Cell-spray auto-grafting technology for deep partial-thickness burns: Problems and solutions during clinical implementation

An in vitro feasibility investigation considering primary human melanocytes for spray- grafting of freshly isolated autologous skin cells for burn treatment and a clinical case report

“…LDH liberation showed a similar trend between pipetted and sprayed cells (Figure 9), As a LDH liberation as a result of a damage occurs immediately after damage, the 21 day values are giving only an indirect information in regard to the LDH liberation behavior of the surviving cells. Case reports of patients in which the cell isolation method was clinically used under an innovative practice approach were already given previously, and we here present anecdotal photo documentation from an Afro-American patient with dark skin coloration [7,8,14,15]. Satisfying results of this one patient with dark skin pigmentation suffering severe second-degree gas explosion burns are shown in ( Figure 10).…”

“…The photo also reveals areas in the neck that represented third-degree burns, which were treated with split skin grafting, but where the cell graft was additionally sprayed into the gaps between the mesh. Information on the cell isolation and the clinical procedure is given in [7,8,14,15]…”

“…Using a previously clinically applied cell isolation method, we have demonstrated that autologous freshly isolated HEK containing skin cell suspensions can be used for immediate spray-grafting with clinically satisfying results [7,8,14,15]. This method differs from the process published by of Wood et al in performing an initial step of separation of the dermal and epidermal layer using the enzyme dispase followed by an extra cell washing step [12].…”

Recent Advances in Enabling Technologies in 3D Printing for Precision Medicine

“…Cell therapies for skin wounds are developing as treatments, demonstrating accelerated wound healing compared to biological skin equivalents. While fast wound coverage has been achieved through delivery of cell suspensions through cell spraying devices, these methods lack the ability to spatially pattern cells for the generation of complex skin structures . Through the design of this in situ printing system, the group aimed to create a portable platform that could be easily transported and sterilized to then identify a variety of wound topologies and deliver multiple cell types in precise, patient‐specific geometries .…”

“…While fast wound coverage has been achieved through delivery of cell suspensions through cell spraying devices, these methods lack the ability to spatially pattern cells for the generation of complex skin structures. [114][115][116] Through the design of this in situ printing system, the group aimed to create a portable platform that could be easily transported and sterilized to then identify a variety of wound topologies and deliver multiple cell types in precise, patient-specific geometries. [114] The main components of the system include a handheld 3D laser scanner to capture an entire wound in one continuous scan, processing software to translate the scan to a 3D print file, and print cartridges for bioinks.…”