Cell‐Surface‐Bound Circulating DNA as a Prognostic Factor in Lung Cancer

Abstract

Since the mortality of lung cancer patients remains very high, development of prognostic methods essential for efficient therapy is an immediate task. This study was designed to assess the value of circulating DNA (cirDNA) in blood as a prognostic marker in patients with non-small cell lung cancer. The average concentration of cirDNA in plasma was shown to be similar in healthy donors and lung cancer patients. However, the concentration of cell-surface-bound circulating DNA (csb-cirDNA) in lung cancer patients is significantly lower than that found in healthy donors (P < 0.0001) and correlates with a poor prognosis of tumor disease. Quantification of the cell-surface-bound DNA in blood of untreated patients allows persons with a poor prognosis of tumor disease to be detected with 94% sensitivity and 50% specificity.

Value of quantitative analysis of circulating cell free DNA as a screening tool for lung cancer: A meta-analysis

“…After full-text review, seven studies [14,15,[25][26][27][28][29] were excluded ( Fig. 1): five articles [14,15,[26][27][28] were excluded because they did not allow the calculation of sensitivity or specificity; and two studies [25,29] were excluded because had significant overlap (the same author, the same institution, time period and some data were repeated) with another study which was better quality. In total, 10 studies [30][31][32][33][34][35][36][37][38][39] including 752 lung cancer patients and 635 healthy controls were available for analysis ( Table 1).…”

“…After independent review, 17 publications [14,15,[25][26][27][28][29][30][31][32][33][34][35][36][37][38][39] dealing with quantitative analysis of circulating plasma DNA for the diagnosis of lung cancer were considered to be eligible for inclusion in the analysis. After full-text review, seven studies [14,15,[25][26][27][28][29] were excluded ( Fig.…”

Characteristics of exosomes andmicroparticles discovered in human tears

“…ДНК 6 образцов здоровых людей элюировали в соответствии с методом, применяемым для удаления ДНК с поверхности клеток [17] или обрабатывали ДНКазой I (1 ед акт/мкл). Ферментативную обработку экзосом СЖ проводили следующим образом: к 460 мкл экзосом добавляли 8 мкл ДНКазы I и 52 мкл 10´буфера для ДНКазы ("Fermentas"), инкубировали в течение 30 мин (37°С), и ингибировали фермент, как описано выше.…”

Biology of Circulating Nucleic Acids and Possible Roles in Diagnosis and Treatment in Diabetes and Cancer

“…Although Tamkovich et al. [188] found the average concentration of circulating DNA in plasma to be similar in healthy donors and lung cancer patients, the concentration of cellsurface-bound circulating DNA was significantly lower for lung cancer patients than for healthy donors (P < 0.0001) so correlating with a poor prognosis of tumor disease. This approach allows the individuals with poor prognosis of tumor disease to be detected with 94 % sensitivity and 50% specificity.…”