Cell Surface Glycosylation Is Required for Efficient Mating of Haloferax volcanii

Shalev, Yarden; Turgeman-Grott, Israela; Tamir, Adi; Eichler, Jerry; Gophna, Uri

doi:10.3389/fmicb.2017.01253

Cited by 43 publications

(78 citation statements)

References 28 publications

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“…It is unclear if pili or other types of lectin molecules are involved in cellular interactions that initiate Haloferax fusion events. Similar to our findings, different Haloferax species are also known to be differentially glycosylated (45) leading to semi-specific cell-cell recognition (44). Cell fusion between different Haloferax species could also be observed but with far lower efficiency (46).…”

Section: Discussionsupporting

confidence: 90%

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Species-specific recognition of Sulfolobales mediated by UV-inducible pili and S-layer glycosylation patterns

Wolferen

Shajahan

Heinrich

et al. 2019

Preprint

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18The UV-inducible pili system of Sulfolobales (Ups) mediates the formation of species-19 specific cellular aggregates. Within these aggregates, cells exchange DNA in order to repair 20 DNA double strand breaks via homologous recombination. Substitution of the S. 21 acidocaldarius pilin subunits UpsA and UpsB with their homologs from Sulfolobus tokodaii 22 42 43 glycan chain (Glc 1 Man 2 GlcNAc 2 QuiS, containing glucose, mannose, N-acetylglucosamine 132 and the Sulfolobus-specific sulfoquinovose residues) (37) (Figure 2). The addition of N-133 acetylgucosamine or glucose did not result in altered cellular aggregation (Figure 2A and B); 134 however, in the presence of mannose, cell aggregates were significantly smaller ( Figure 2B). 135This suggests that the mannose molecules partially saturate the binding sites of the Ups-pili 136 and thereby inhibit interactions between pili and the glycan chains on the S-layer of the host 137 cell resulting in reduced aggregation. 138Defining the glycosylation pattern of S. tokodaii S-layer proteins 139

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Section: Discussionsupporting

confidence: 90%

“…Among the euryarchaeal Haloferax species, glycosylation was found to be essential for cell fusion (44), emphasizing the importance of glycosylation in Archaeal cellular recognition in general. It is unclear if pili or other types of lectin molecules are involved in cellular interactions that initiate Haloferax fusion events.…”

Section: Discussionmentioning

confidence: 99%

Species-specific recognition of Sulfolobales mediated by UV-inducible pili and S-layer glycosylation patterns

Wolferen

Shajahan

Heinrich

et al. 2019

Preprint

View full text Add to dashboard Cite

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“…Indeed, only recently, S-layer glycoproteins of hyperthermophilic archaea have been shown to accumulate iron and sulfur from the environment 27,28 . Also, recent studies revealed S-layer glycosylation to be crucial for cell-to-cell interactions during the mating process of the archaeon Haloferax 29 and comparative genomics suggest a within-niche variation of glycosylation enabling Haloferax to discriminate between such mating partners and also viruses 30 . Similarly, virus-host interaction between Halorubrum sp.…”

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confidence: 99%

CRISPR-mediated gene silencing reveals involvement of the archaeal S-layer in cell division and virus infection

et al. 2019

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The S-layer is a proteinaceous surface lattice found in the cell envelope of bacteria and archaea. In most archaea, a glycosylated S-layer constitutes the sole cell wall and there is evidence that it contributes to cell shape maintenance and stress resilience. Here we use a gene-knockdown technology based on an endogenous CRISPR type III complex to gradually silence slaB, which encodes the S-layer membrane anchor in the hyperthermophilic archaeon Sulfolobus solfataricus. Silenced cells exhibit a reduced or peeled-off S-layer lattice, cell shape alterations and decreased surface glycosylation. These cells barely propagate but increase in diameter and DNA content, indicating impaired cell division; their phenotypes can be rescued through genetic complementation. Furthermore, S-layer depleted cells are less susceptible to infection with the virus SSV1. Our study highlights the usefulness of the CRISPR type III system for gene silencing in archaea, and supports that an intact S-layer is important for cell division and virus susceptibility.

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“…Among the euryarchaeal Haloferax species, glycosylation was found to be essential for cell fusion (49), emphasizing the importance of glycosylation in archaeal cellular recognition in general. It is unclear if pili or other types of lectin molecules are involved in cellular interactions that initiate Haloferax fusion events.…”

Section: Discussionmentioning

confidence: 99%

Species-Specific Recognition of Sulfolobales Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns

Wolferen

Shajahan

Heinrich

et al. 2020

mBio

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The UV-inducible pili system of Sulfolobales (Ups) mediates the formation of species-specific cellular aggregates. Within these aggregates, cells exchange DNA to repair DNA double-strand breaks via homologous recombination. Substitution of the Sulfolobus acidocaldarius pilin subunits UpsA and UpsB with their homologs from Sulfolobus tokodaii showed that these subunits facilitate species-specific aggregation. A region of low conservation within the UpsA homologs is primarily important for this specificity. Aggregation assays in the presence of different sugars showed the importance of N-glycosylation in the recognition process. In addition, the N-glycan decorating the S-layer of S. tokodaii is different from the one of S. acidocaldarius. Therefore, each Sulfolobus species seems to have developed a unique UpsA binding pocket and unique N-glycan composition to ensure aggregation and, consequently, also DNA exchange with cells from only the same species, which is essential for DNA repair by homologous recombination. IMPORTANCE Type IV pili can be found on the cell surface of many archaea and bacteria where they play important roles in different processes. The UV-inducible pili system of Sulfolobales (Ups) pili from the crenarchaeal Sulfolobales species are essential in establishing species-specific mating partners, thereby assisting in genome stability. With this work, we show that different Sulfolobus species have specific regions in their Ups pili subunits, which allow them to interact only with cells from the same species. Additionally, different Sulfolobus species have unique surface-layer N-glycosylation patterns. We propose that the unique features of each species allow the recognition of specific mating partners. This knowledge for the first time gives insights into the molecular basis of archaeal self-recognition.

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Cell Surface Glycosylation Is Required for Efficient Mating of Haloferax volcanii

Cited by 43 publications

References 28 publications

Species-specific recognition of Sulfolobales mediated by UV-inducible pili and S-layer glycosylation patterns

Species-specific recognition of Sulfolobales mediated by UV-inducible pili and S-layer glycosylation patterns

CRISPR-mediated gene silencing reveals involvement of the archaeal S-layer in cell division and virus infection

Species-Specific Recognition of Sulfolobales Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns

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