Cell surface phenotyping and cytokine production of Epstein–Barr Virus (EBV)-transformed lymphoblastoid cell lines (LCLs)

Wroblewski, Joanne M.; Copple, Angela; Batson, Lydia P.; Landers, Cheri; Yannelli, John R.

doi:10.1016/s0022-1759(01)00565-8

Cited by 34 publications

(29 citation statements)

References 36 publications

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“…According to previous studies, EBV transformation of primary human B cells changes various cellular properties (23,24), such as the expression of the cell activation-associated Ag CD38 (25), and the expression of iNOS (26). Furthermore, B-LCLs have been described as expressing various cytokines with a high degree of heterogeneity (24,(27)(28)(29)(30)(31)(32). In our B-LCL, iNOS expression was detectable in highly activated CD38 ++ EBV-infected B cells, but not in CD38 + or CD38 2 B cells (Fig.…”

Section: Inos-expressing B Lineage Cells Produce Pro-and Antiinflammamentioning

confidence: 99%

Mucosal Inducible NO Synthase–Producing IgA+ Plasma Cells in Helicobacter pylori–Infected Patients

Neumann

Mueller

Moos

et al. 2016

The Journal of Immunology

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The mucosal immune system is relevant for homeostasis, immunity, and also pathological conditions in the gastrointestinal tract. Inducible NO synthase (iNOS)–dependent production of NO is one of the factors linked to both antimicrobial immunity and pathological conditions. Upregulation of iNOS has been observed in human Helicobacter pylori infection, but the cellular sources of iNOS are ill defined. Key differences in regulation of iNOS expression impair the translation from mouse models to human medicine. To characterize mucosal iNOS-producing leukocytes, biopsy specimens from H. pylori–infected patients, controls, and participants of a vaccination trial were analyzed by immunohistochemistry, along with flow cytometric analyses of lymphocytes for iNOS expression and activity. We newly identified mucosal IgA-producing plasma cells (PCs) as one major iNOS+ cell population in H. pylori–infected patients and confirmed intracellular NO production. Because we did not detect iNOS+ PCs in three distinct infectious diseases, this is not a general feature of mucosal PCs under conditions of infection. Furthermore, numbers of mucosal iNOS+ PCs were elevated in individuals who had cleared experimental H. pylori infection compared with those who had not. Thus, IgA+ PCs expressing iNOS are described for the first time, to our knowledge, in humans. iNOS+ PCs are induced in the course of human H. pylori infection, and their abundance seems to correlate with the clinical course of the infection.

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Section: Inos-expressing B Lineage Cells Produce Pro-and Antiinflammamentioning

confidence: 99%

Mucosal Inducible NO Synthase–Producing IgA+ Plasma Cells in Helicobacter pylori–Infected Patients

Neumann

Mueller

Moos

et al. 2016

The Journal of Immunology

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“…These methods appear physiologic in evaluating memory T cell responses, avoiding the addition of exogenous materials and long term tissue culture (20,27,28). For example, EBV-transformed LCL in long term culture can secrete various cytokines, including IL-10, thereby potentially affecting cytotoxicity results (44,45). Conversely, the T cells responsible for cytotoxicity are the ones that produce cytokines, in particular Th1 cytokines such as IFN-␥ or TNF-␣ (the ones measured using the short term stimulation assay in our study (33)), with the frequency of these cells correlated with EBV viral loads (24,41,46,47).…”

Section: Of Ebv-specific T Cells In Patients With Sle and Healthy Conmentioning

confidence: 99%

Defective Control of Latent Epstein-Barr Virus Infection in Systemic Lupus Erythematosus

Kang

Quan

Nolasco

et al. 2004

The Journal of Immunology

233

187

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EBV infection is more common in patients with systemic lupus erythematosus (SLE) than in control subjects, suggesting that this virus plays an etiologic role in disease and/or that patients with lupus have impaired EBV-specific immune responses. In the current report we assessed immune responsiveness to EBV in patients with SLE and healthy controls, determining virus-specific T cell responses and EBV viral loads using whole blood recall assays, HLA-A2 tetramers, and real-time quantitative PCR. Patients with SLE had an ∼40-fold increase in EBV viral loads compared with controls, a finding not explained by disease activity or immunosuppressive medications. The frequency of EBV-specific CD69+ CD4+ T cells producing IFN-γ was higher in patients with SLE than in controls. By contrast, the frequency of EBV-specific CD69+ CD8+ T cells producing IFN-γ in patients with SLE appeared lower than that in healthy controls, although this difference was not statistically significant. These findings suggest a role for CD4+ T cells in controlling, and a possible defect in CD8+ T cells in regulating, increased viral loads in lupus. These ideas were supported by correlations between viral loads and EBV-specific T cell responses in lupus patients. EBV viral loads were inversely correlated with the frequency of EBV-specific CD69+ CD4+ T cells producing IFN-γ and were positively correlated with the frequencies of CD69+ CD8+ T cells producing IFN-γ and with EBV-specific, HLA-A2 tetramer-positive CD8+ T cells. These results demonstrate that patients with SLE have defective control of latent EBV infection that probably stems from altered T cell responses against EBV.

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“…In a study of 37 EBV-transformed B lymphoblastoid cell lines, 11 (30%) of the cell lines were negative for pan-B-cell markers CD19 and CD20. 17 Also, it has been reported that up to 80% of the EBER-positive cells in the peripheral blood exhibit features of plasmacytoid differentiation. 18 In the present study, numerous BZLF1-positive cells were detected within the tonsils, primarily within the lymphocyterich crypt epithelium.…”

Section: Ebv In Tonsils Sd Hudnall Et Almentioning

confidence: 99%

Distribution and phenotype of Epstein–Barr virus-infected cells in human pharyngeal tonsils

et al. 2005

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Although Epstein-Barr virus (EBV) is often found in human tonsils, it remains to be precisely determined in what cells and microenvironment the virus is present. Although generally regarded as a B lymphotropic virus, EBV is associated with non-B-cell tumors, for example, NK/T-cell lymphoma, carcinoma, and leiomyosarcoma. To provide a basis for understanding the origin and biology of EBV-infected non-B cells, the immunophenotype of all EBV-infected cells in reactive human tonsils was determined by subjecting tonsil sections to dual/triple EBER in situ hybridization and immunohistochemistry with monoclonal antibodies to T cells (CD3, CD4, CD8,

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Cell surface phenotyping and cytokine production of Epstein–Barr Virus (EBV)-transformed lymphoblastoid cell lines (LCLs)

Cited by 34 publications

References 36 publications

Mucosal Inducible NO Synthase–Producing IgA+ Plasma Cells in Helicobacter pylori–Infected Patients

Mucosal Inducible NO Synthase–Producing IgA+ Plasma Cells in Helicobacter pylori–Infected Patients

Defective Control of Latent Epstein-Barr Virus Infection in Systemic Lupus Erythematosus

Distribution and phenotype of Epstein–Barr virus-infected cells in human pharyngeal tonsils

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