Cell-to-Cell Interaction in the Immune Response

Anderson, Robert E.; Sprent, J.; Miller, J. F. A. P.

doi:10.1084/jem.135.3.711

Cited by 25 publications

(2 citation statements)

References 13 publications

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“…In contradistinction with B cells, considerable controversy exists with re spect to the radiosensitivity of T cells, especially helper T cells (Anderson et al, 1972Janeway, 1975;Miller and Mitchell, 1968;Claman and Chaperon, general, unprimed T cells are radiosensitive when assayed in vivo or in vitro. Primed T cells, on the other hand, tend to be radiosensitive when assayed in an adoptive transfer system but resistant when assayed in vitro or irradiated in situ.…”

Section: Immunoglobulin In Vivomentioning

confidence: 99%

Effects of Irradiation on the <i>in vitro </i>Immune Response

Anderson¹,

Lefkovits²

1980

Pathobiology

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Primed and unprimed murine spleen cells and suspensions enriched for T or B cells were exposed to various doses of radiation in vitro and evaluated with respect to their capacity to respond to sheep red blood cells (SRBC) in 1-ml or 10-µl cultures. The dose-response data show three components: (1) a low dose ( < 50 rad) phase associated with an augmented anti-SRBC response; (2) a radiosensitive phase associated with a precipitous loss of anti-SRBC activity; (3) a relative radioresistant phase with a loss of anti-SRBC activity which occurs over a broad range of radiation doses. Data obtained utilizing irradiated subpopulations enriched for T or B cells combined with excess numbers of nonirradiated lymphocytes of the corresponding cell type suggest that low dose augmentation is the result of radiation-induced injury to T cells or subcomponent thereof. With respect to the second component of the dose-response curve, similar experiments show that B cells are more radiosensitive than T cells (D₃₇ of ∼ 80 vs. ∼220 rad, respectively) in this response. The third component of the curve appears to involve the interaction of radioresistant subpopulations of T and B cells.

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Section: Immunoglobulin In Vivomentioning

confidence: 99%

Effects of Irradiation on the <i>in vitro </i>Immune Response

Anderson¹,

Lefkovits²

1980

Pathobiology

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“…Helper T cells in mice have been found radioresis tant in some studies [17,18], whereas others have found helper effects to be radiosensitive [19,20], In part, these discrepancies relate to whether the assay is carried out in vivo or in vitro, but the functional state of the irradiated cells appears to be equally impor tant. Helper cells leaving primed peripheral lymph nodes of mice have never been looked at and we see no controversy in the finding that helper cells leaving lymph nodes of sheep synthesize DNA and possibly proliferate elsewhere.…”

Section: Discussionmentioning

confidence: 98%

The Appearance of Specific Helper Cells in Lymph of Immunized Sheep

Heron¹,

Cahill²,

Trnka³

1982

Int Arch Allergy Immunol

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Culture conditions which support the induction by antigen of plaque-forming cells (PFCs) in cell suspensions made from lymph nodes from sheep do not support the induction of PFCs in suspensions of non-primed lymphocytes obtained from the efferent lymph of a lymph node. The lymphocytes in the efferent lymph of a node after immunization with heterologous erythrocyte antigen, via an afferent lymphatic vessel, however, respond in vitro to the specific antigen with the generation of PFCs. Immune responses were confined to single lymph nodes, and the lymphocytes of the efferent lymph issuing from these were added to nonimmunized efferent lymph cells of other nodes in the same sheep. It was found that the addition of low numbers of immunized T lymphocytes to saturating numbers of nonimmune lymphocytes, either unseparated, or the B cell fraction only, gave rise in cultures stimulated with the specific antigen to PFCs in numbers comparable to those induced in cultures containing only saturating amounts of immunized lymph node lymphocytes. Immunized B cells were not capable of providing help. The specific helper T cells required were found to be recirculating between blood and lymph for several months after immunization. Irradiated T cells were not capable of providing sufficient help. The number of lymphocytes forming rosettes with chicken red blood cells or horse red blood cells in efferent lymph increases after immunization of a sheep lymph node through an afferent lymphatic vessel with specific antigens. Rosette-forming cells (RFCs) were removed by Ficoll-Isopaque flotation and the properties of the RFCs were determined in the culture system. It was found that specific B precursor cells were antigen binding because the response in vitro was abolished after these rosette-forming B cells had been removed. Helper T cells appearing in the efferent lymph subsequent to immunization were found not to be antigen binding because T cells issuing from immunized lymph nodes, after removal of RFCs, were still capable of providing specific help to a nonimmunized B cell population.

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The enhancement of a primary in vitro anti‐hapten response by carrier priming

Trowbridge

1972

Eur J Immunol

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Abbreviations: NIP: 4-Hydroxy-3-iodo-5-nitrophenacetyl NIP-Az: 4-Hydroxy-3-iodo-5-nitrophenacethyl azide RBC: Red blood cell SRBC: Sheep red blood cell RRBC: Rabbit red blood cell CRBC: forming cells TNP: 2,4,6-Trinitrophenyl dThd: Thymidine [ 'HjdThd: (6-3H]thymidine NMS: Normal mouse serum BSS: Balanced salt solution Chicken red DRBC: red PFC: response [ 2-81, but in order to analyze these cellular events Eur. J. Immunol. 1972.2: 558-565 A primary in vitro anti-hapten response 559

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Cell-to-Cell Interaction in the Immune Response

Cited by 25 publications

References 13 publications

Effects of Irradiation on the <i>in vitro </i>Immune Response

Effects of Irradiation on the <i>in vitro </i>Immune Response

The Appearance of Specific Helper Cells in Lymph of Immunized Sheep

The enhancement of a primary in vitro anti‐hapten response by carrier priming

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