Cell-to-cell movement of three genera (+) ss RNA plant viruses

Otulak, Katarzyna; Garbaczewska, Grażyna

doi:10.1007/s11738-010-0538-2

Cited by 8 publications

(4 citation statements)

References 109 publications

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“…Cell-to-cell movement via PDs is essential for a plant virus to establish a systemic infection (Harries & Ding, 2011). Plant viruses encode a class of proteins, so-called movement proteins (MPs), which increase the size exclusion limit (SEL) of PDs and interact with virions or viral genomes to execute viral cell-to-cell movement (Wolf et al , 1989; Otulak & Garbaczewska, 2011).…”

Section: Full Textmentioning

confidence: 99%

Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement

Ishikawa

Maejima

Komatsu

et al. 2013

Journal of General Virology

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Fig mosaic virus (FMV), a member of the newly formed genus Emaravirus, is a segmented negative-strand RNA virus. Each of the six genomic FMV segments contains a single ORF: that of RNA4 encodes the protein p4. FMV-p4 is presumed to be the movement protein (MP) of the virus; however, direct experimental evidence for this is lacking. We assessed the intercellular distribution of FMV-p4 in plant cells by confocal laser scanning microscopy and we found that FMV-p4 was localized to plasmodesmata and to the plasma membrane accompanied by tubulelike structures. A series of experiments designed to examine the movement functions revealed that FMV-p4 has the capacity to complement viral cell-to-cell movement, prompt GFP diffusion between cells, and spread by itself to neighbouring cells. Altogether, our findings demonstrated that FMV-p4 shares several properties with other viral MPs and plays an important role in cell-tocell movement.

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Section: Full Textmentioning

confidence: 99%

Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement

Ishikawa

Maejima

Komatsu

et al. 2013

Journal of General Virology

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“…Therefore, a viral expression system, combined with the appropriate host plant, could provide an efficient tool for studying protein functions and producing valuable secondary metabolites in planta without having to generate stable transgenic plants. While plant viral vectors have been widely used to down-regulate the expression of endogenous genes [4-9], they have been also developed to over-express heterologous proteins [3,10-16]. However, there can be limitations associated with the different viral systems used.…”

Section: Introductionmentioning

confidence: 99%

A Narcissus mosaic viral vector system for protein expression and flavonoid production

et al. 2013

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BackgroundWith the explosive numbers of sequences generated by next generation sequencing, the demand for high throughput screening to understand gene function has grown. Plant viral vectors have been widely used as tools in down-regulating plant gene expression. However, plant viral vectors can also express proteins in a very efficient manner and, therefore, can also serve as a valuable tool for characterizing proteins and their functions in metabolic pathways in planta.ResultsIn this study, we have developed a Gateway®-based high throughput viral vector cloning system from Narcissus Mosaic Virus (NMV). Using the reporter genes of GFP and GUS, and the plant genes PAP1 (an R2R3 MYB which activates the anthocyanin pathway) and selenium-binding protein 1 (SeBP), we show that NMV vectors and the model plant Nicotiana benthamiana can be used for efficient protein expression, protein subcellular localization and secondary metabolite production.ConclusionsOur results suggest that not only can the plant viral vector system be employed for protein work but also can potentially be amenable to producing valuable secondary metabolites on a large scale, as the system does not require plant regeneration from seed or calli, which are stages where certain secondary metabolites can interfere with development.

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“…The long-distance transport of viruses is closely related to the transport that occurs from the phloem parenchyma cells or accompanying cells to the interior parts of the sieve elements in the phloem, where the virus moves rapidly (several centimeters per hour). Then, the virus is actively transported together with assimilates inside the sieve elements [ 63 , 64 , 65 , 66 ]. Less frequent systemic transport involves the tracheal elements of xylem.…”

Section: The Specificity Of Cell-to-cell Transport In Different Plmentioning

confidence: 99%

Molecular Biology of Prune Dwarf Virus—A Lesser Known Member of the Bromoviridae but a Vital Component in the Dynamic Virus–Host Cell Interaction Network

Kozieł

Bujarski

Otulak

2017

IJMS

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Prune dwarf virus (PDV) is one of the members of Bromoviridae family, genus Ilarvirus. Host components that participate in the regulation of viral replication or cell-to-cell movement via plasmodesmata are still unknown. In contrast, viral infections caused by some other Bromoviridae members are well characterized. Bromoviridae can be distinguished based on localization of their replication process in infected cells, cell-to-cell movement mechanisms, and plant-specific response reactions. Depending upon the genus, “genome activation” and viral replication are linked to various membranous structures ranging from endoplasmic reticulum, to tonoplast. In the case of PDV, there is still no evidence of natural resistance sources in the host plants susceptible to virus infection. Apparently, PDV has a great ability to overcome the natural defense responses in a wide spectrum of plant hosts. The first manifestations of PDV infection are specific cell membrane alterations, and the formation of replicase complexes that support PDV RNA replication inside the spherules. During each stage of its life cycle, the virus uses cell components to replicate and to spread in whole plants, within the largely suppressed cellular immunity environment. This work presents the above stages of the PDV life cycle in the context of current knowledge about other Bromoviridae members.

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Cell-to-cell movement of three genera (+) ss RNA plant viruses

Cited by 8 publications

References 109 publications

Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement

Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement

A Narcissus mosaic viral vector system for protein expression and flavonoid production

Molecular Biology of Prune Dwarf Virus—A Lesser Known Member of the Bromoviridae but a Vital Component in the Dynamic Virus–Host Cell Interaction Network

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