Cell-type diversity in the bed nucleus of the stria terminalis to regulate motivated behaviors

Ortiz-Juza, Maria Magdalena; Alghorazi, Rizk; Rodríguez-Romaguera, Jose

doi:10.1016/j.bbr.2021.113401

Cited by 17 publications

(20 citation statements)

References 97 publications

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“…Similar to the CeAL, we find that the majority of BNST Nts, Crh, and Tac2 expressing neurons are GABAergic; however, very few express all three neuropeptides. These results are consistent with single cell sequencing of the BNST that identified Nts and Crh expressing neurons as largely distinct (Rodriguez-Romaguera et al, 2020;Ortiz-Juza et al, 2021). Here, we find that ∼12% of BNST neurons express both Crh and Nts, indicating that they are predominantly nonoverlapping populations.…”

Section: Discussionsupporting

confidence: 90%

Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTA

Soden

Yee

Cuevas

et al. 2022

Front. Neural Circuits

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Neuropeptides play an important role in modulating mesolimbic system function. However, while synaptic inputs to the ventral tegmental area (VTA) have been extensively mapped, the sources of many neuropeptides are not well resolved. Here, we mapped the anatomical locations of three neuropeptide inputs to the VTA: neurotensin (NTS), corticotrophin releasing factor (CRF), and neurokinin B (NkB). Among numerous labeled inputs we identified the bed nucleus of the stria terminalis (BNST) as a major source of all three peptides, containing similar numbers of NTS, CRF, and NkB VTA projection neurons. Approximately 50% of BNST to VTA inputs co-expressed two or more of the peptides examined. Consistent with this expression pattern, analysis of calcium dynamics in the terminals of these inputs in the VTA revealed both common and distinct patterns of activation during appetitive and aversive conditioning. These data demonstrate additional diversification of the mesolimbic dopamine system through partially overlapping neuropeptidergic inputs.

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Section: Discussionsupporting

confidence: 90%

Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTA

Soden

Yee

Cuevas

et al. 2022

Front. Neural Circuits

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“…In addition, these results contrast with another study that showed that inactivation of BNST reduced TMT-induced fear-related behavior (Fendt et al, 2003). The BNST is composed of several genetically identifiable cell-types (Giardino and Pomrenze, 2021; Ortiz-Juza et al, 2021), therefore, one explanation for why our inactivation of one cell-type in the BNST did not reduce TMT fear-related behavior could be that a different cell-type is responsible for TMT induced fear-related behavior. Another possible explanation for our finding could be that bulk inhibition of Pnoc BNST neurons is not selective enough to modulate behavior.…”

Section: Discussionmentioning

confidence: 89%

“…We previously found using single-cell sequencing that Pnoc BNST neurons co-express other genetic markers such as somatostatin ( Som ), protein kinase C δ ( Pkcδ ), cholecystokinin ( Cck ) (Rodriguez-Romaguera et al, 2020). Each of these genetic markers anatomically cluster in distinct nuclei within the BNST (Ortiz-Juza et al, 2021), therefore, it is possible that the diverse response in Pnoc BNST activity dynamics could be attributed to subtypes within the Pnoc BNST neuronal population. Thus, bulk inhibition of the collective Pnoc BNST neuronal population may not have been sufficient to modulate the specific neuronal ensemble responsible for driving the motivated behavior to aversive stimuli.…”

Section: Discussionmentioning

confidence: 99%

“…The bed nucleus of the stria terminalis (BNST) is a genetically diverse hub of the extended amygdala (Giardino and Pomrenze, 2021; Ortiz-Juza et al, 2021), and is strongly implicated in human psychopathologies defined by alterations in arousal and motivational states (Straube et al, 2007; Walker et al, 2009; Yassa et al, 2012). Recently, we identified that the activity of a subpopulation of prepronociceptin -expressing neurons within the BNST ( Pnoc BNST neurons) directly correlates with rapid changes in physiological arousal (observed by increases in pupillary size) when mice are presented with motivationally salient predator and food odors (Rodriguez-Romaguera et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Prepronociceptin-expressing neurons in the extended amygdala signal darting away from an aversive odor

Ung

Ortiz-Juza

Curtis

et al. 2022

Preprint

Self Cite

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Dysregulation in the neural circuitry that encodes physiological arousal responses is thought to contribute to the manifestation of the maladaptive behaviors observed in neuropsychiatric disorders. We previously found that prepronociceptin-expressing neurons in the bed nucleus of the stria terminalis (PnocBNST neurons) modulate rapid changes in physiological arousal upon presentation of motivationally salient stimuli (Rodriguez-Romaguera et al., 2020). However, whether PnocBNST neurons are necessary to regulate behavioral actions to motivationally salient stimuli is still unknown. Here, we investigated the role of PnocBNST neurons in encoding behavioral responses to motivationally salient stimuli using in vivo calcium imaging and optogenetic approaches in freely behaving mice. We find that the bulk activity of PnocBNST neurons increases when mice are near an aversive odor in comparison to a rewarding odor. However, optogenetic inhibition of PnocBNST neurons does not affect the amount of time mice spend near an aversive odor. Further analysis revealed that a subgroup of PnocBNST neurons that correlate with proximity to the aversive odor also correlate to darting away from the same aversive odor. Since these two behaviors are opposite to each other and since we previously found PnocBNST neurons correlate with arousal responses, we believe these results may be due in part to the encoding of arousal responses that occur when mice approach and dart away from aversive stimuli.

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“…The complexity of BNST is not only valid for its subnuclear division, but also for its neurochemical profile, types of neurons and their connections, functions and gene expression [5,6,12,14,26,32,34]. The main types of neurons in BNST are GABAergic, mainly seen in the oval nucleus [28,36].…”

Section: Discussionmentioning

confidence: 99%

The Bed Nucleus of the Stria Terminalis: Cytoarchitecture and Morphometry of its Subnuclear Organizationin the rat Brain

Gradev¹,

Jelev²,

Lazarov³

2022

AMA

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The complexity of the bed nucleus of the stria terminalis (BNST), its function, sex difference, cytoand chemoarchitectonic structure has intrigued scientists during the last decades. As part of the extended amygdala, it is involved in many limbic functions being the main target of treating anxiety and addiction as lately revealed. This paper is a brief review of the BNST structure and also a morphometric study of its subnuclear groups in the adult male rat brain. The Kluver-Barrera staining revealed the main white matter tracts which serve us for landmarks to identify the BNST subdivisions. The majority of BNST neurons were small in size, less than 15µm in diameter, oval in shape and with prominent nucleoli. The present results clearly show the complexity in the BNST structural organization to better understand its function, and to emphasize it as a novel research area of the functional morphology.

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Cell-type diversity in the bed nucleus of the stria terminalis to regulate motivated behaviors

Cited by 17 publications

References 97 publications

Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTA

Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTA

Prepronociceptin-expressing neurons in the extended amygdala signal darting away from an aversive odor

The Bed Nucleus of the Stria Terminalis: Cytoarchitecture and Morphometry of its Subnuclear Organizationin the rat Brain

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