Cell type-specific adaptation of cellular and nuclear volume in micro-engineered 3D environments

Greiner, Alexandra M.; Klein, Friederike; Gudzenko, Tetyana; Richter, Benjamin; Striebel, Thomas; Wundari, Bayu G.; Autenrieth, Tatjana J.; Wegener, Martin; Franz, Clemens M.; Bastmeyer, Martin

doi:10.1016/j.biomaterials.2015.08.016

Cited by 46 publications

(32 citation statements)

References 85 publications

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“…Although the proliferation and adhesion were similar to conventional 2D culture conditions, the cells largely differed with respect to their morphology. Strikingly, fibroblasts, but not epithelial cells, almost doubled their cellular volume when cultured in 3D . This is consistent to their in vivo origin, where fibroblasts grow in a complex 3D environment whereas epithelial cells form a planar tissue.…”

Section: Direct Laser Writing For Cell Culturesupporting

confidence: 77%

3D Scaffolds to Study Basic Cell Biology

et al. 2019

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Mimicking the properties of the extracellular matrix is crucial for developing in vitro models of the physiological microenvironment of living cells. Among other techniques, 3D direct laser writing (DLW) has emerged as a promising technology for realizing tailored 3D scaffolds for cell biology studies. Here, results based on DLW addressing basic biological issues, e.g., cell‐force measurements and selective 3D cell spreading on functionalized structures are reviewed. Continuous future progress in DLW materials engineering and innovative approaches for scaffold fabrication will enable further applications of DLW in applied biomedical research and tissue engineering.

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Section: Direct Laser Writing For Cell Culturesupporting

confidence: 77%

3D Scaffolds to Study Basic Cell Biology

et al. 2019

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“…For instance, the volume‐sensitive Cl − channel has been found to play an important role in both cell volume regulation (Lee et al, ; Shimizu, ) and cisplatin resistance of human cancer cells. In addition, accumulating evidence has shown some other influence factors on cell volume (Bush, Hodkinson, Hamilton, & Hall, ; Greiner et al, ; Marino & La Spada, ; Turunen et al, ). For instance, the volume of chondrocytes reduces up to 30% under compressive force loading (Bush et al, ).…”

Section: Introductionmentioning

confidence: 99%

The effect of substrate stiffness on cancer cell volume homeostasis

Wang

Chai

Sha

et al. 2017

Journal Cellular Physiology

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Existing studies on the mechanism of cell volume regulation are mainly relevant to ion channels and osmosis in extracellular fluid. Recently, accumulating evidence has shown that cellular mechanical microenvironment also influences the cell volume. Herein, we investigated the regulation of substrate stiffness on the cell volume homeostasis of MCF-7 cells and their following migration behaviors. We found that cell volume increases with increasing substrate stiffness, which could be affected by blocking the cell membrane anion permeability and dopamine receptor. In addition, the cell migration is significantly inhibited by decreasing the cell volume using tamoxifen and such inhibition effect on migration is enhanced by increasing substrate stiffness. The cell membrane anion permeability might be the linker between cellular mechanical microenvironment and cellular volume homeostasis regulation. This work revealed the regulation of substrate stiffness on cell volume homeostasis for the first time, which would provide a new perspective into the understanding of cancer metastasis and a promising anti-cancer therapy through regulation of cell volume homeostasis.

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“…[8][9][10] Im letzten Jahrzehnt hat sich hier das DLWals ein leistungsfähiges Verfahren erwiesen, um zelluläre 3D-Mikroumgebungen mit wohldefinierter Geometrie herzustellen. In einem Großteil dieser Studien wurden Gerüststrukturen, bestehend aus einem einzelnen Photolack mit einer homogenen Oberflächenbeschichtung aus Biomolekülen, verwendet, um Zellwachstum, [11,12] Zelladhäsion, [13,14] Zellmigration [15,16] oder Stammzelldifferenzierung [17] in definierten 3D-Mikroumgebungen zu untersuchen. In letzter Zeit sind mehrere exzellente Übersichtsartikel über die relevante Literatur erschienen und wir verweisen auf die dort zitierten Arbeiten.…”

Section: Zellkulturanwendungenunclassified

3D‐Laser‐Mikro‐Nanodruck: Herausforderungen für die Chemie

et al. 2017

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3D‐Druck ist eine leistungsfähige Technik für die maßgeschneiderte Herstellung funktionaler Materialien. Dieser Aufsatz fasst den Stand der Technik im Hinblick auf 3D‐Laser‐Mikro‐ und ‐Nanodruck zusammen und erkundet die chemischen Herausforderungen, die derzeit die volle Etablierung dieser Technologie limitieren: von der Entwicklung fortgeschrittener Materialien für Anwendungen in der Zellbiologie und der Elektronik bis hin zu den bestehenden chemischen Grenzen des schnellen Schreibens mit Auflösungen unterhalb der Beugungsgrenze. Des Weiteren untersuchen wir Möglichkeiten zur Realisierung des direkten Laserschreibens mehrerer Materialien aus einem Photolack heraus, basierend auf wellenlängenselektiven photochemischen Prozessen (λ‐Orthogonalität). Schließlich betrachten wir chemische Prozesse, mit deren Hilfe adaptive 3D‐Strukturen geschrieben werden können, die auf externe Stimuli wie Licht, Wärme, pH‐Wert oder spezifische Moleküle reagieren, sowie fortgeschrittene Konzepte für abbaubare Stützstrukturen.

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Cell type-specific adaptation of cellular and nuclear volume in micro-engineered 3D environments

Cited by 46 publications

References 85 publications

3D Scaffolds to Study Basic Cell Biology

3D Scaffolds to Study Basic Cell Biology

The effect of substrate stiffness on cancer cell volume homeostasis

3D‐Laser‐Mikro‐Nanodruck: Herausforderungen für die Chemie

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