Cell wall antigens in soluble cytoplasmic extracts of Candida albicans as demonstrated by crossed immuno-affinoelectrophoresis with Concanavalin A

Syverson, Rae Ellen; Buckley, Helen R.

doi:10.1016/0022-1759(77)90166-1

Cited by 18 publications

(7 citation statements)

References 18 publications

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“…Cytokines and chemokines were quantified by capture ELISA (BD Pharmingen, San Diego, CA, USA) according to manufacturer’s instructions and as previously described 15,16 . Absorbance was read at 450 nm using an automated plate reader (Bio‐Tek Instruments, Winooski, VT, USA).…”

Section: Methodsmentioning

confidence: 99%

Susceptibility of Middle Adolescent Females to Sexually Transmitted infections: Impact of Hormone Contraception and Sexual Behaviors on Vaginal Immunity

Barousse

Theall

Pol

et al. 2007

American J Rep Immunol

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Section: Methodsmentioning

confidence: 99%

Susceptibility of Middle Adolescent Females to Sexually Transmitted infections: Impact of Hormone Contraception and Sexual Behaviors on Vaginal Immunity

Barousse

Theall

Pol

et al. 2007

American J Rep Immunol

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“…The technique of crossed-immunoelectrophoresis with Concanavalin A was used to demonstrate the precipitation of four antigens from soluble cy-toplasmic extracts of the yeast and mycelial phases of C. albicans, all of them glycoproteins (172). Two were also present in spheroplasts, being associated with cytoplasmic antigens, while the other two were related to the cell wall.…”

Section: Candida Albicansmentioning

confidence: 99%

The cell wall of fungal human pathogens: Its possible role in host-parasite relationshipsA review

San-Blas

1982

Mycopathologia

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The ability of some fungi to cause disease in animals, and particularly in humans, appears to be related to some peculiar trait of their metabolism not shared by taxonomically related species, so that among thousands of species of fungi, only some 100 are considered pathogenic for humans.Recently, there has been an increase in the basic research aimed at elucidating the physiology, biochemistry, and mechanisms of pathogenicity of the fungi in addition to the responses of the infected host. The results from these studies are often difficult to compare because of the lack of reference cultures, and because variability among laboratories are found for many parameters such as origin of isolates, growth conditions, and fractionation procedures used to obtain chemically defined components of cell walls.This review deals with cell wall structures and their influence in host-parasite relationships in those relatively few pathogenic fungi on which substantial literature exists. Serological properties of the walls are included when available. Readers interested in medical and biological aspects of fungi may consult Rippon's (140) and Emmons et al.'s (51) textbooks, among others. Some reviews on microbial surfaces and their relationships to pathogenicity have been published (160 162)2The pathogenic fungi included in this review are classified according to their cell wall structure, following Bartnicki-Garcia's criteria (15) on the distribution of the two major polysaccharides within the fungal cell wall. This classification is based on the fact that fungi may be subdivided into various categories according to the chemical nature of their walls, and that these categories closely parallel conventional taxonomic boundaries. As seen in Table l, this classification is based on dual combinations of those polysaccharides which appear to be the principal component of vegetative walls, disregarding the presence of small amounts of other classifying polysaccharides. The few pathogenic fungi whose cell walls have been studied to some extent belong to Bartnicki-Garcia's categories 5 (chitin-glucan) and 6 (mannan-glucan) ( Table 2). Besides these main polysaccharides, some minor components of the cell wall may also play important roles in pathogenicity, as antigens, or as virulence factors, and they will be mentioned in this review. DermatophytesDermatophytes are a highly specialized group of fungi that, through long evolutionary processes became adapted to invade, colonize, and nourish themselves on the keratinized tissues of animals. The three genera of dermatophytes are: Trichophyton, Microsporum, and Epidermophyton, of which 21, 15, and 1 species, respectively, are currently considered valid members (1) (ln the Vth Panamerican Conference on Mycoses, April 1980, Caracas, these figures were raised to 27, 16, and 2, respectively). Mating studies have revealed that, for some cases, what were considered as single species of Microsporum and Trichophyton, actually represent complexes of more than one species, indis- Mycopathologia7...

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“…different batches of cells was tested against sera from patients with systemic candidiasis by using crossed immunoaffinoelectrophoresis in the presence of concanavalin A (ConA) (13) and was found to be reproducible. The MCE was then passed through ConA-Sepharose 4B (Pharmacia, Uppsala, Sweden) affinity columns to remove cell wall mannan, glucan, and mannan-glucan complexes.…”

Section: Methodsmentioning

confidence: 99%

Production and characterization of three monoclonal antibodies to Candida albicans proteins

Strockbine¹,

Largen²,

Buckley³

1984

Infect Immun

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Three monoclonal antibodies, designated A2C7, C2C7, and F19, were produced which recognize proteins from Candida albicans. All are of the immunoglobulin GI heavy chain and kappa light chain class. A2C7 and C2C7 immunoprecipitated three proteins contained in a partially purified fraction (region A) of a mycelial cytoplasmic extract of C. albicans. The apparent molecular weights of these proteins are 120,000 (120K) to 135K, 44K to 52K, and 35K to 38K. Monoclonal antibody F19 was reactive with proteins of 42K, 43K, and 50K in immunoblotting experiments. F19 was also able to form a precipitin band in agarose gel with protein(s) contained in region A. Limited proteolytic digestion of the three proteins immunoprecipitated by A2C7 and C2C7 demonstrated that both monoclonal antibodies recognized the same three Candida proteins and that there exists a significant degree of relatedness in primary structure among the three proteins. Proteins with apparent molecular weights of 120K to 135K, 44K to 52K, and 35K to 38K that were immunoprecipitated by sera from two patients with invasive candidiasis and by the serum from a rabbit immunized against a 48K (44K to 52K) Candida protein were also analyzed by limited proteolysis. Patterns of peptide fragments generated by enzymatic digestion of these proteins showed that the proteins recognized by the monoclonal antibodies are the same proteins recognized by antibodies in the sera of patients during an invasive Candida infection and by antibodies in the serum of the immune rabbit.

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Cell wall antigens in soluble cytoplasmic extracts of Candida albicans as demonstrated by crossed immuno-affinoelectrophoresis with Concanavalin A

Cited by 18 publications

References 18 publications

Susceptibility of Middle Adolescent Females to Sexually Transmitted infections: Impact of Hormone Contraception and Sexual Behaviors on Vaginal Immunity

Susceptibility of Middle Adolescent Females to Sexually Transmitted infections: Impact of Hormone Contraception and Sexual Behaviors on Vaginal Immunity

The cell wall of fungal human pathogens: Its possible role in host-parasite relationshipsA review

Production and characterization of three monoclonal antibodies to Candida albicans proteins

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