Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced

Kuczak, Michał; Kurczyńska, Ewa

doi:10.3390/ijms21218126

Cited by 14 publications

(18 citation statements)

References 102 publications

(171 reference statements)

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“…Thus, recent reviews [44,45] cover numerous papers that demonstrate specific AGP distribution, particularly in metabolically active tissues involved in virtually all aspects of plant growth from Bryophytes onwards [46]. AGPs mark critical cell fate transitions [47] in both sporophyte and gametophyte generations. Thus, judging from extensive cytochemical studies, seed germination, vegetative growth, flowering, fertilization [48], embryogenesis [49][50][51], phyllotaxis [52] and fruit ripening [53] all involve AGPs.…”

Section: Indirect Evidence For the Role Of Agps In Ca 2+ Homeostasismentioning

confidence: 99%

A Molecular Pinball Machine of the Plasma Membrane Regulates Plant Growth—A New Paradigm

Lamport

Kieliszewski

2021

Cells

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Novel molecular pinball machines of the plasma membrane control cytosolic Ca2+ levels that regulate plant metabolism. The essential components involve: 1. an auxin-activated proton pump; 2. arabinogalactan glycoproteins (AGPs); 3. Ca2+ channels; 4. auxin-efflux “PIN” proteins. Typical pinball machines release pinballs that trigger various sound and visual effects. However, in plants, “proton pinballs” eject Ca2+ bound by paired glucuronic acid residues of numerous glycomodules in periplasmic AGP-Ca2+. Freed Ca2+ ions flow down the electrostatic gradient through open Ca2+ channels into the cytosol, thus activating numerous Ca2+-dependent activities. Clearly, cytosolic Ca2+ levels depend on the activity of the proton pump, the state of Ca2+ channels and the size of the periplasmic AGP-Ca2+ capacitor; proton pump activation is a major regulatory focal point tightly controlled by the supply of auxin. Auxin efflux carriers conveniently known as “PIN” proteins (null mutants are pin-shaped) pump auxin from cell to cell. Mechanosensitive Ca2+ channels and their activation by reactive oxygen species (ROS) are yet another factor regulating cytosolic Ca2+. Cell expansion also triggers proton pump/pinball activity by the mechanotransduction of wall stress via Hechtian adhesion, thus forming a Hechtian oscillator that underlies cycles of wall plasticity and oscillatory growth. Finally, the Ca2+ homeostasis of plants depends on cell surface external storage as a source of dynamic Ca2+, unlike the internal ER storage source of animals, where the added regulatory complexities ranging from vitamin D to parathormone contrast with the elegant simplicity of plant life. This paper summarizes a sixty-year Odyssey.

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Section: Indirect Evidence For the Role Of Agps In Ca 2+ Homeostasismentioning

confidence: 99%

A Molecular Pinball Machine of the Plasma Membrane Regulates Plant Growth—A New Paradigm

Lamport

Kieliszewski

2021

Cells

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“…Here, the results are discussed by focusing on three crucial pathways, given their documented role in the determination of plant cell fate [101,102] and because it would be too broad to treat in detail all the gene ontologies enriched at 0D. Here, the focus is given to base excision repair-and cell wall-related processes, as well as photosynthesisrelated ontologies.…”

Section: Transcripts Related With the Metabolism Of Organic Acids And Cytokininsmentioning

confidence: 99%

Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis

Xuan

Legay

Berni

et al. 2021

IJMS

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Callogenesis, the process during which explants derived from differentiated plant tissues are subjected to a trans-differentiation step characterized by the proliferation of a mass of cells, is fundamental to indirect organogenesis and the establishment of cell suspension cultures. Therefore, understanding how callogenesis takes place is helpful to plant tissue culture, as well as to plant biotechnology and bioprocess engineering. The common herbaceous plant stinging nettle (Urtica dioica L.) is a species producing cellulosic fibres (the bast fibres) and a whole array of phytochemicals for pharmacological, nutraceutical and cosmeceutical use. Thus, it is of interest as a potential multi-purpose plant. In this study, callogenesis in internode explants of a nettle fibre clone (clone 13) was studied using RNA-Seq to understand which gene ontologies predominate at different time points. Callogenesis was induced with the plant growth regulators α-napthaleneacetic acid (NAA) and 6-benzyl aminopurine (BAP) after having determined their optimal concentrations. The process was studied over a period of 34 days, a time point at which a well-visible callus mass developed on the explants. The bioinformatic analysis of the transcriptomic dataset revealed specific gene ontologies characterizing each of the four time points investigated (0, 1, 10 and 34 days). The results show that, while the advanced stage of callogenesis is characterized by the iron deficiency response triggered by the high levels of reactive oxygen species accumulated by the proliferating cell mass, the intermediate and early phases are dominated by ontologies related to the immune response and cell wall loosening, respectively.

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“…Thus recent reviews [44,45] cover numerous papers that demonstrate specific AGP distribution particularly in metabolically active tissues involved in virtually all aspects of plant growth from Bryophytes onwards [46]. AGPs mark critical cell fate transitions [47] in both sporophyte and gametophyte generations. Thus, judging from extensive cytochemical studies, seed germination, vegetative growth, flowering, fertilisation [48], embryogenesis [49,50,51], phyllotaxis [52] and fruit ripening [53] all involve AGPs.…”

Section: Indirect Evidence For the Role Of Agps In Ca 2+ Homeostasismentioning

confidence: 99%

A Molecular Pinball Machine of the Plasma Membrane Regulates Plant Growth – A New Paradigm

Lamport¹,

Tan²,

Kieliszewski

2021

Preprint

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Novel molecular pinball machines of the plasma membrane control cytosolic Ca2+ levels that regulate plant metabolism. [https://youtu.be/zABg7LiBk88] Essential components involve: 1. an auxin-activated proton pump; 2. arabinogalactan glycoproteins (AGPs); 3. Ca2+ channels; 4. auxin-efflux “PIN” proteins. Typical pinball machines release pinballs that trigger various sound and visual effects. However, in plants “proton pinballs” eject Ca2+ bound by paired glucuronic acid residues of numerous glycomodules in periplasmic AGP-Ca2+. Freed Ca2+ ions flow down the electrostatic gradient through open Ca2+ channels into the cytosol thus activating numerous Ca2+-dependent activities.Clearly cytosolic Ca2+ levels depend on activity of the proton pump, the state of Ca2+ channels and size of the periplasmic AGP-Ca2+ capacitor: Proton pump activation is a major regulatory focal point tightly controlled by the supply of auxin: auxin efflux carriers conveniently known as “PIN” proteins [null mutants are pin-shaped!] pump auxin from cell to cell. Mechanosensitive Ca2+ channels and their activation by reactive oxygen species (ROS) are yet another factor regulating cytosolic Ca2+.Cell expansion also triggers proton pump/pinball activity by mechanotransduction of wall stress via Hechtian adhesion thus forming a Hechtian oscillator that underlies cycles of wall plasticity and oscillatory growth.Finally, Ca2+ homeostasis of plants depends on cell surface external storage as source of dynamic Ca2+, unlike the internal ER storage source of animals where the added regulatory complexities ranging from vitamin D to parathormone contrast with the elegant simplicity of plant life. This paper summarises a sixty year Odyssey.

show abstract

Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced

Cited by 14 publications

References 102 publications

A Molecular Pinball Machine of the Plasma Membrane Regulates Plant Growth—A New Paradigm

A Molecular Pinball Machine of the Plasma Membrane Regulates Plant Growth—A New Paradigm

Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis

A Molecular Pinball Machine of the Plasma Membrane Regulates Plant Growth – A New Paradigm

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