Cell wall compositional and vascular traits of hybrid poplar wood in micropropagated plants and plants propagated from root cuttings

Ďurkovič, Jaroslav; Kačík, František; Husárová, Hana; Mamoňová, Miroslava; Čaňová, Ingrid

doi:10.1007/s11056-019-09723-y

Cited by 5 publications

(2 citation statements)

References 51 publications

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“…Interestingly, the necrotic tissue was eventually sequestered, and Phytophthora hyphae were prevented from penetrating deeper into the healthy living tissues. Compared to other poplar trees grown in the area, the wood of the hybrid poplar clone T-14 contained a lower amount of lignin and a higher amount of cellulose ( Ďurkovič et al., 2017 ; 2020 ). However, the mechanisms of Phytophthora tolerance have not been reported.…”

Section: Introductionmentioning

confidence: 93%

Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar

et al. 2022

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Poplars are among the fastest-growing trees and significant resources in agriculture and forestry. However, rapid growth requires a large water consumption, and irrigation water provides a natural means for pathogen spread. That includes members of Phytophthora spp. that have proven to be a global enemy to forests. With the known adaptability to new hosts, it is only a matter of time for more aggressive Phytophthora species to become a threat to poplar forests and plantations. Here, the effects of artificial inoculation with two different representatives of aggressive species (P. cactorum and P. plurivora) were analyzed in the proteome of the Phytophthora-tolerant hybrid poplar clone T-14 [Populus tremula L. 70 × (Populus × canescens (Ait.) Sm. 23)]. Wood microcore samples were collected at the active necrosis borders to provide insight into the molecular processes underlying the observed tolerance to Phytophthora. The analysis revealed the impact of Phytophthora on poplar primary and secondary metabolism, including carbohydrate-active enzymes, amino acid biosynthesis, phenolic metabolism, and lipid metabolism, all of which were confirmed by consecutive metabolome and lipidome profiling. Modulations of enzymes indicating systemic response were confirmed by the analysis of leaf proteome, and sampling of wood microcores in distal locations revealed proteins with abundance correlating with proximity to the infection, including germin-like proteins, components of proteosynthesis, glutamate carboxypeptidase, and an enzyme that likely promotes anthocyanin stability. Finally, the identified Phytophthora-responsive proteins were compared to those previously found in trees with compromised defense against Phytophthora, namely, Quercus spp. and Castanea sativa. That provided a subset of candidate markers of Phytophthora tolerance, including certain ribosomal proteins, auxin metabolism enzymes, dioxygenases, polyphenol oxidases, trehalose-phosphate synthase, mannose-1-phosphate guanylyltransferase, and rhamnose biosynthetic enzymes. In summary, this analysis provided the first insight into the molecular mechanisms of hybrid poplar defense against Phytophthora and identified prospective targets for improving Phytophthora tolerance in trees.

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Section: Introductionmentioning

confidence: 93%

Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar

et al. 2022

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“…(ii) to identify the emissions of volatile organic compounds (VOCs) that are induced by the Phytophthora isolates and which may contribute to plant defence responses. Recently, T-14 plants micropropagated in tissue culture showed a better performance for leaf growth traits, dissipation energy of midrib xylem conduit cell walls, and cellulose content than the plants propagated conventionally from root cuttings [34,35]. Artificial inoculations of P. cactorum and P. plurivora isolates on hybrid poplar plants were performed on 20th June 2017, i.e., at day 0, using the underbark inoculation test at breast height.…”

Section: Introductionmentioning

confidence: 99%

Effects of Phytophthora Inoculations on Photosynthetic Behaviour and Induced Defence Responses of Plant Volatiles in Field-Grown Hybrid Poplar Tolerant to Bark Canker Disease

Ďurkovič

Bubeníková

Gužmerová

et al. 2021

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Bark cankers accompanied by symptoms of decline and dieback are the result of a destructive disease caused by Phytophthora infections in woody plants. Pathogenicity, gas exchange, chlorophyll a fluorescence, and volatile responses to P. cactorum and P. plurivora inoculations were studied in field-grown 10-year-old hybrid poplar plants. The most stressful effects of P. cactorum on photosynthetic behaviour were found at days 30 and 38 post-inoculation (p.-i.), whereas major disturbances induced by P. plurivora were identified at day 30 p.-i. and also belatedly at day 52 p.-i. The spectrum of volatile organic compounds emitted at day 98 p.-i. was richer than that at day 9 p.-i, and the emissions of both sesquiterpenes α-cubebene and germacrene D were induced solely by the Phytophthora inoculations. Significant positive relationships were found between both the axial and the tangential development of bark cankers and the emissions of α-cubebene and β-caryophyllene, respectively. These results show that both α-cubebene and germacrene D are signal molecules for the suppression of Phytophthora hyphae spread from necrotic sites of the bark to healthy living tissues. Four years following inoculations, for the majority of the inoculated plants, the callus tissue had already closed over the bark cankers.

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Salinity-driven changes in Salicornia cell wall nanomechanics and lignin composition

Cárdenas Pérez,

Strzelecki,

Piernik

et al. 2024

Environmental and Experimental Botany

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Cell wall compositional and vascular traits of hybrid poplar wood in micropropagated plants and plants propagated from root cuttings

Cited by 5 publications

References 51 publications

Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar

Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar

Effects of Phytophthora Inoculations on Photosynthetic Behaviour and Induced Defence Responses of Plant Volatiles in Field-Grown Hybrid Poplar Tolerant to Bark Canker Disease

Salinity-driven changes in Salicornia cell wall nanomechanics and lignin composition

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