Cellobiose oxidase crosslinked in a redox polymer matrix at an electrode surface—a new biosensor

Elmgren, Maja; Lindquist, Sten‐Eric; Henriksson, Gunnar

doi:10.1016/0022-0728(92)80487-o

Cited by 44 publications

(31 citation statements)

References 20 publications

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“…Poly(1-vinylimidazole) 12 -[Os(4,4-dimethyl-2,2-dipyridyl) 2 Cl 2 ] 2þ/þ (osmium redox polymer) was dissolved in Milli-Q water at a concentration of 10 mg/mL. A premixed solution including 2.0 mL of redox polymer (2 pg), 1.0 mL of a freshly prepared poly(ethylene glycol) diglycidyl (PEGDGE) solution (2.5 mg/mL in water) was placed on top of the polished end of the electrode and spread evenly using the microsyringe tip.…”

Section: Preparation Of the Biosensorsmentioning

confidence: 99%

“…Some of them have been previously used for biosensor constructions, e.g., hexose oxidase [7], oligosaccharide dehydrogenase [8 -10], aldose dehydrogenase [11], cellobiose dehydrogenase [12,13], carbohydrate oxidase [14] and pyranose oxidase [15,16]. In the present work, electrical wiring of various forms of two different sugar oxidizing enzymes, i.e., pyranose oxidase (P2O), both wild type and a mutant, as well as one commercial P2O, and additionally pyranose dehydrogenase (PDH) from two different origins, with an osmium redox polymer, (poly(1-vinylimidazole) 12 -[Os(4,4'-dimethyl-2,2'-dipyridyl) 2 Cl 2 ] 2þ/þ ), is reported. Both P2O and PDH are not anomer specific as they do not oxidize the sugar at the C-1.…”

Section: Introductionmentioning

confidence: 99%

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Amperometric Biosensors for Detection of Sugars Based on the Electrical Wiring of Different Pyranose Oxidases and Pyranose Dehydrogenases with Osmium Redox Polymer on Graphite Electrodes

et al. 2007

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Electrical wiring of different types of pyranose oxidase (P2O) (fungal wild type, recombinant wild type with a hexahistidine tag, mutant form E542K with a hexa-histidine tag) from Trametes multicolor, and recombinant P2O from Coriolus sp. overexpressed in Escherichia coli as well as of pyranose dehydrogenase (PDH) from Agaricus meleagris and Agaricus xanthoderma with an osmium redox polymer (poly(1-vinylimidazole) 12 -[Os(4,4'-dimethyl-2,2'-dipyridyl) 2 -Cl 2 ] 2þ/þ ) on graphite electrodes was carried out. After optimization studies using glucose as substrate, the biosensors, which showed the best characteristics in terms of linear range, detection limit and sensitivity were selected, viz. wild type P2O from T. multicolor and PDH from A. meleagris. These two enzymes were used and investigated for their selectivity for a number of different sugars.

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Section: Preparation Of the Biosensorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Amperometric Biosensors for Detection of Sugars Based on the Electrical Wiring of Different Pyranose Oxidases and Pyranose Dehydrogenases with Osmium Redox Polymer on Graphite Electrodes

et al. 2007

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“…A number of these have been used in conjunction mainly with analytical flow systems for the detection of some mono-, di-, and oligosaccharides. Examples of such enzymes are oligosaccharide dehydrogenase [6][7][8], aldose dehydrogenase [9], cellobiose dehydrogenase (formerly denoted cellobiose oxidase) [7,10], and hexose oxidase [11]. In this article, yet another enzyme with a broad enzyme specificity is used, viz.…”

Section: Introductionmentioning

confidence: 98%

Pyranose Oxidase Modified Carbon Paste Electrodes for Monosaccharide Determination

et al. 1998

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An amperometric biosensor for the detection of some monosaccharides was developed with co-immobilized pyranose oxidase (purified from the basidiomycete fungus Phanerochaete chrysosporium) and horseradish peroxidase in carbon paste. Due to rather low selectivity of pyranose oxidase, the biosensor could detect both predominating anomers of D-glucose as well as the substrates D-xylose, D-galactose, and d-gluconolactone. The best sensor performance was obtained from a carbon paste with covalently immobilized enzymes together with the additives lactitol and polyethylenimine, resulting in a sensitivity for glucose of 30.2 mA cm ¹2 mM ¹1 . The enzyme modified electrode was investigated in an automated flow injection system at the operating potential of ¹50 mV (vs. Ag/AgCl) with an injection frequency of 40 h ¹1 . The biosensor was also integrated as the detection unit in a liquid chromatographic system for the detection of monosaccharides to show its potential use in real applications.

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“…Since CDH has several attractive properties, e.g., its specificity for ␤-1,4-linked disaccharides, the increased availability of the S. rolfsii enzyme could enable a range of technological applications, such as biosensors, bioremediation, or biocatalysis. CDH has been used in colorimetric assays (12) and in amperometric biosensors (19) for the detection of lactose.…”

mentioning

confidence: 99%

Purification and Characterization of Cellobiose Dehydrogenase from the Plant PathogenSclerotium(Athelia)rolfsii

Baminger

Subramaniam

Renganathan

et al. 2001

Appl Environ Microbiol

122

108

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Cellobiose dehydrogenase (CDH) is an extracellular hemoflavoenzyme produced by several wood-degrading fungi. In the presence of a suitable electron acceptor, e.g., 2,6-dichloro-indophenol (DCIP), cytochrome c, or metal ions, CDH oxidizes cellobiose to cellobionolactone. The phytopathogenic fungus Sclerotium rolfsii (teleomorph: Athelia rolfsii) strain CBS 191.62 produces remarkably high levels of CDH activity when grown on a cellulose-containing medium. Of the 7,500 U of extracellular enzyme activity formed per liter, less than 10% can be attributed to the proteolytic product cellobiose:quinone oxidoreductase. As with CDH from wood-rotting fungi, the intact, monomeric enzyme from S. rolfsii contains one heme b and one flavin adenine dinucleotide cofactor per molecule. It has a molecular size of 101 kDa, of which 15% is glycosylation, and a pI value of 4.2. The preferred substrates are cellobiose and cellooligosaccharides; additionally, ␤-lactose, thiocellobiose, and xylobiose are efficiently oxidized. Cytochrome c (equine) and the azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid) cation radical were the best electron acceptors, while DCIP, 1,4-benzoquinone, phenothiazine dyes such as methylene blue, phenoxazine dyes such as Meldola's blue, and ferricyanide were also excellent acceptors. In addition, electrons can be transferred to oxygen. Limited in vitro proteolysis with papain resulted in the formation of several protein fragments that are active with DCIP but not with cytochrome c. Such a flavin-containing fragment, with a mass of 75 kDa and a pI of 5.1 and lacking the heme domain, was isolated and partially characterized.

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Cellobiose oxidase crosslinked in a redox polymer matrix at an electrode surface—a new biosensor

Cited by 44 publications

References 20 publications

Amperometric Biosensors for Detection of Sugars Based on the Electrical Wiring of Different Pyranose Oxidases and Pyranose Dehydrogenases with Osmium Redox Polymer on Graphite Electrodes

Amperometric Biosensors for Detection of Sugars Based on the Electrical Wiring of Different Pyranose Oxidases and Pyranose Dehydrogenases with Osmium Redox Polymer on Graphite Electrodes

Pyranose Oxidase Modified Carbon Paste Electrodes for Monosaccharide Determination

Purification and Characterization of Cellobiose Dehydrogenase from the Plant PathogenSclerotium(Athelia)rolfsii

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