Cells and material-based strategies for regenerative endodontics

Siddiqui, Zain; Acevedo‐Jake, Amanda M.; Griffith, Alexandra; Kadincesme, Nurten; Dabek, Kinga; Hindi, Dana; Kim, Ka Kyung; Kobayashi, Yoshifumi; Shimizu, Emi; Kumar, Vivek

doi:10.1016/j.bioactmat.2021.11.015

Cited by 31 publications

(38 citation statements)

References 185 publications

(332 reference statements)

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“…The skull is a flat bone, which is similar to other maxillofacial bone structures, with vascular structure inside and rich trabeculae on the surface, but no definite bone marrow cavity structure 124 , 125 . Unlike bone, teeth are directly exposed to the oral environment, and their internal vascular network only interacts with the outside through the root canal orifice; thus, their ability to self-repair is limited 4 , 126 . The existence of vital pulp forms the basis for maintaining the biological activity of the pulp–dentin complex and is vital for the preservation and continuous development of young permanent teeth whose apical foramen is not completely closed 127 .…”

Section: Discussionmentioning

confidence: 99%

Biomineralization-inspired mineralized hydrogel promotes the repair and regeneration of dentin/bone hard tissue

et al. 2023

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Maxillofacial hard tissue defects caused by trauma or infection often affect craniofacial function. Taking the natural hard tissue structure as a template, constructing an engineered tissue repair module is an important scheme to realize the functional regeneration and repair of maxillofacial hard tissue. Here, inspired by the biomineralization process, we constructed a composite mineral matrix hydrogel PAA-CMC-TDM containing amorphous calcium phosphates (ACPs), polyacrylic acid (PAA), carboxymethyl chitosan (CMC) and dentin matrix (TDM). The dynamic network composed of Ca2+·COO− coordination and ACPs made the hydrogel loaded with TDM, and exhibited self-repairing ability and injectability. The mechanical properties of PAA-CMC-TDM can be regulated, but the functional activity of TDM remains unaffected. Cytological studies and animal models of hard tissue defects show that the hydrogel can promote the odontogenesis or osteogenic differentiation of mesenchymal stem cells, adapt to irregular hard tissue defects, and promote in situ regeneration of defective tooth and bone tissues. In summary, this paper shows that the injectable TDM hydrogel based on biomimetic mineralization theory can induce hard tissue formation and promote dentin/bone regeneration.

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Section: Discussionmentioning

confidence: 99%

Biomineralization-inspired mineralized hydrogel promotes the repair and regeneration of dentin/bone hard tissue

et al. 2023

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“…The human teeth are composed of organized, mineralized tissue layers of dentin, cementum and enamel. In native tooth architecture, an enamel-encased crown surrounds the internal pulp chamber and roots [2].…”

Section: Particularities Of Oral Cavitymentioning

confidence: 99%

“…In healthy tooth anatomy, the dentin-pulp complex lies below a continuous layer of ordered enamel, protecting the vessel and nerve rich pulp [2].…”

Section: Particularities Of Oral Cavitymentioning

confidence: 99%

“…Berberine and Icariin promote odontoblastic and odontogenic differentiation, they are both responsible of the inhibition of alveolar bone osteoporosis and promote periodontal bone regeneration [43] [82] [95]- [102], Drynaria iisos increases bone cell viability [103] [104], Rhizoma drynariae enhances the proliferation of hmscs [2] [103] [104], Foeniculum vulgare promotes differentiation of hMSCs into osteoblasts [104], Ferula gummosa has antibacterial effects and induces proliferation of hMSCs into osteocytes [105], Resveratrol induces proliferation and differentiation of articular cartilage, enhances bone healing by its anti-inflammatory properties, responsible for the inhibition of boneloss and increases the blood supply [104] [106]- [111]. Fructus ligustri lucidi increases osteogenesis stimulating genes [112] [113].…”

Section: Action On Alveolar Bonementioning

confidence: 99%

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An Overview of the Use of Medicinal Plants in Regenerative Dentistry

Dhoum¹,

Ibenmoussa²,

Sidqui³

2023

OJST

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Aim: The oral cavity has the particularity to host multiple hard and soft tissues, in this paper, we will discuss the current therapies that lead to cell differentiation by regenerative therapies and the future alternatives proposed by medicinal plants and all the regenerative potential of these different tissues. Material and Methods: A detailed review of the literature through the various search engines: Scopus, PubMed, google scholar, Cochrane, etc., uses the selected keywords to explore the effect of the regenerative potential of several medicinal plants. Results: Through our research, we have proceeded to sort different medicinal plants, according to their repairing and regenerative potential on the different tissues of the oral cavity. Conclusion: Future studies are conceivable to explore the opportunities and potential provided by medicinal plants in the field of regenerative dentistry.

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“…Human dental pulp stem cells (hDPSCs) are a heterogeneous population of cells isolated from human permanent third molar pulp. In recent years, these ready-made stem cells have been found to have important applications in dentistry and other fields [ 1 ]. Extensive expansion of human DPSCs in vitro is a prerequisite for basic research and clinical development.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of adrenomedullin (ADM) alleviates the senescence of human dental pulp stem cells by regulating the miR-152/CCNA2 pathway

et al. 2022

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The limitation of human dental pulp stem cells (DPSCs), which have potential application value in regenerative medicine, is that they are prone to age in vitro. Studies have shown adrenomedullin (ADM) is believed to promote the proliferation of human DPSCs, but whether it can also affect aging remains to be investigated. A lentivirus vector was used to construct human DPSCs overexpressing ADM. Senescence tests were carried out on cells of the 7th and 15th passage. Transcriptome analysis was conducted to analyze microRNA expression regulation changes after human DPSCs overexpressed ADM. H 2 O 2 induced the aging model of human DPSCs, and we examined the mechanism of recovery of aging through transfection experiments with miR-152 mimic, pCDH-CCNA2, and CCNA2 siRNA. Overexpression of ADM significantly upregulated the G2/M phase ratio of human DPSCs in natural passage culture (P = 0.001) and inhibited the expression of p53 (P = 0.014), P21 WAF1 (P = 0.015), and P16 INK4A (P = 0.001). Decreased ROS accumulation was observed in human DPSCs during long-term natural passage (P = 0.022). Transcriptome analysis showed that miR-152 was significantly upregulated during human DPSC senescence (P = 0.001) and could induce cell senescence by directly targeting CCNA2. Transfection with miR-152 mimic significantly reversed the inhibitory effect of ADM overexpression on p53 (P = 0.006), P21 WAF1 (P = 0.012), and P16 INK4A (P = 0.01) proteins in human DPSCs (H 2 O 2 -induced). In contrast, pCDH-CCNA2 weakened the effect of the miR-152 mimic, thus promoting cell proliferation and antiaging. ADM-overexpressing human DPSCs promote cell cycle progression and resist cellular senescence through CCNA2 expression promotion by inhibiting miR-152.

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Cells and material-based strategies for regenerative endodontics

Cited by 31 publications

References 185 publications

Biomineralization-inspired mineralized hydrogel promotes the repair and regeneration of dentin/bone hard tissue

Biomineralization-inspired mineralized hydrogel promotes the repair and regeneration of dentin/bone hard tissue

An Overview of the Use of Medicinal Plants in Regenerative Dentistry

Overexpression of adrenomedullin (ADM) alleviates the senescence of human dental pulp stem cells by regulating the miR-152/CCNA2 pathway

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