Cells Disruption by Ultrasonication

Discovery of plant chemical defence mediated by a two-component system involving β-glucosidase in Panax species

“…PnGH1 triggers hydrolysis upon cell disruption in fresh healthy PNLs, as demonstrated by a bead milling experiment. A similar result was found in an aqueous extract of dried PNLs following ultrasonication 10,18 , an efficient method for disrupting cell walls and membranes 39 . Furthermore, massive cell disruption can be elicited by fungal infection.…”

Bacterial cell wall material properties determine E. coli resistance to sonolysis

“…Sonication is widely used for dispersing, surface cleaning, degassing, food and beverage processing, medical scanning, nano synthesis, mineral processing, welding, cell disruption, and extraction of cellular components [1] , [2] , [3] , [4] , [5] , [6] . Another promising potential of sonication is disruption and inactivation of bacteria, for example in wastewater treatment plants [7] .…”

A Novel Strategy for Extraction of Intracellular Poly(3-Hydroxybutyrate) From Engineered Pseudomonas Putida Using Deep Eutectic Solvents: Comparison with Traditional Biobased Organic Solvents

“…However, as sonication is energy intensive, it could have a negative impact on the polymer chain length, and has scaling problems, it was determined that the disruption without sonication was effective enough to cause high levels of disruption. Hence, sonication was left out of the pre-treatment procedure [44]. Lysozyme could be used in bulk processes to disrupt cells immediately after fermentation, and it does not require any energy input to carry out the disruption unlike high pressure homogenization.…”