Cells Involved in the Immune Response

Cells Involved in the Immune Response

“…The experimental protocols carried out in the present communication were designed wholly on the basis of the results presented previously by this laboratory (1)(2)(3)(4). It had previously been demonstrated that (a) the bone marrow cells of normal rabbits are capable of undergoing blastogenesis and mitosis, of incorporating tritiated thymidine, and of synthesizing antibody in response to the stimulus provided by a large number of antigenic materials (1,2). (b) The bone marrow cells of immunized rabbits lose this in vitro reactivity when exposed to the specific immunizing antigen, but are as reactive as normal bone marrow cells to other, non-cross-reacting antigens (1,2).…”

“…It had previously been demonstrated that (a) the bone marrow cells of normal rabbits are capable of undergoing blastogenesis and mitosis, of incorporating tritiated thymidine, and of synthesizing antibody in response to the stimulus provided by a large number of antigenic materials (1,2). (b) The bone marrow cells of immunized rabbits lose this in vitro reactivity when exposed to the specific immunizing antigen, but are as reactive as normal bone marrow cells to other, non-cross-reacting antigens (1,2). (c) This in vitro reactivity of the bone marrow cells to an antigen was lost very shortly after the intravenous administration of the antigen, the eclipse or latent period not exceeding 8-24 hr when a relatively large quantity of the antigen was administered (i.e., 25 mg human serum albumin [HSA], 10 nag keyhole limpet hemocyanin [KLH]).…”

“…Normal, primed, or immune rabbits were sacrificed by the intravenous injection of Nembutal. The bone marrow from both the tibia and femur was removed and suspended in sterile normal rabbit serum (NRS) (Microbiological Associates) in sterile plastic tubes (Falcon Plastics, Los Angeles, Calif.) and shaken vigorously for 1-2 rain to obtain a ceil suspension, as has been previously described (1)(2)(3). The tubes were centrifuged at 600 rpm for 10 rain; the fatty upper layer was decanted and the cells resuspended in Medium 199.…”

“…(Received for publication 12 December 1968) In previous communications from this laboratory (1,2) it was demonstrated that bone marrow cells of normal rabbits are capable of undergoing blastogenesis and mitosis upon incubation with a large number of antigens in vitro. Antibody formation by the bone marrow cells in vitro was detected by the fluorescent antibody technique (2).…”