Cells of extraembryonic mesodermal origin confer human dystrophin in the Mdx model of duchenne muscular dystrophy

Kawamichi, Yayoi; Cui, Chang-Hao; Toyoda, Masashi; Makino, Hidenari; Horie, Akane; Takahashi, Yoriko; Matsumoto, Kenji; Saito, Hirohisa; Ohta, Hiroaki; Saito, Kayoko; Umezawa, Akihiro

doi:10.1002/jcp.22076

Cited by 27 publications

(23 citation statements)

References 21 publications

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“…In this study, we only expanded MSCs from placental tissues from uncomplicated pregnancies. However, we also revealed different characteristics among CP-MSCs, CV-MSCs and DB-MSCs, supporting the idea of heterogeneity and tissue-specificity among placental MSCs for cell properties and proliferative capacities13424454. We have started to expand and characterize placental MSCs from abnormal conditions (e.g.…”

Section: Discussionsupporting

confidence: 72%

“…These explants produced adequate numbers of cells with high proliferative potency. Numerous methods have been used to isolate/expand MSCs from placental tissue, including enzymatic digestion of tissues to harvest MSCs as a single-cell suspension for further cell expansion4344, or by seeding tissue fragments, as in the current study. Contamination between maternal- and foetal-origin MSCs remains a problem with expansion of MSCs from placental tissues4546, but we were able to generate pure cells without contamination.…”

Section: Discussionmentioning

confidence: 99%

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Feasibility of placenta-derived mesenchymal stem cells as a tool for studying pregnancy-related disorders

Fuchi

Miura

Doi

et al. 2017

Sci Rep

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The cellular and molecular mechanisms responsible for pregnancy-related disorders remain unclear. We investigated the feasibility of using placenta-derived mesenchymal stem cells (MSCs) as a tool to study such pregnancy-related disorders. We isolated and expanded adequate numbers of cells with characteristic features of MSCs from the chorionic plate (CP-MSCs), chorionic villi (CV-MSCs), and decidua basalis (DB-MSCs) of human term placental tissues. All placenta-derived MSCs expressed pregnancy-associated C14MC microRNA (miRNA) (miR-323-3p). Interestingly, the placenta-specific C19MC miRNAs (miR-518b and miR517a) were clearly expressed in CP-MSCs and CV-MSCs of foetal origin, but were barely expressed in DB-MSCs of maternal origin. Furthermore, expression levels of placenta-specific C19MC miRNAs in CV-MSCs remained stable during the ex vivo expansion process and across different pregnancy phases (first trimester versus third trimester). High-efficiency siRNA transfection was confirmed in twice-passaged CV-MSCs with little toxicity, and microarray analysis was used to screen for miR-518b target genes. Placenta-derived MSCs, especially CV-MSCs, are a potential tool for investigating the role of placental miRNAs in pregnancy-related disorders.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Feasibility of placenta-derived mesenchymal stem cells as a tool for studying pregnancy-related disorders

Fuchi

Miura

Doi

et al. 2017

Sci Rep

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“…Cells-Endometrium (UtE) (29), placental artery endothelium (PAE) (30), and amnion (AM) (31,32) were independently established. UtE, AM, and MRC5 cells were maintained in the POWEREDBY10 medium (MED SHIROTORI CO., Ltd.).…”

Section: Methodsmentioning

confidence: 99%

Glycome Diagnosis of Human Induced Pluripotent Stem Cells Using Lectin Microarray

Tateno

Toyota

Saito

et al. 2011

Journal of Biological Chemistry

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196

224

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Induced pluripotent stem cells (iPSCs) can now be produced from various somatic cell (SC) lines by ectopic expression of the four transcription factors. Although the procedure has been demonstrated to induce global change in gene and microRNA expressions and even epigenetic modification, it remains largely unknown how this transcription factor-induced reprogramming affects the total glycan repertoire expressed on the cells. Here we performed a comprehensive glycan analysis using 114 types of human iPSCs generated from five different SCs and compared their glycomes with those of human embryonic stem cells (ESCs; nine cell types) using a high density lectin microarray. In unsupervised cluster analysis of the results obtained by lectin microarray, both undifferentiated iPSCs and ESCs were clustered as one large group. However, they were clearly separated from the group of differentiated SCs, whereas all of the four SCs had apparently distinct glycome profiles from one another, demonstrating that SCs with originally distinct glycan profiles have acquired those similar to ESCs upon induction of pluripotency. Thirty-eight lectins discriminating between SCs and iPSCs/ESCs were statistically selected, and characteristic features of the pluripotent state were then obtained at the level of the cellular glycome. The expression profiles of relevant glycosyltransferase genes agreed well with the results obtained by lectin microarray. Among the 38 lectins, rBC2LCN was found to detect only undifferentiated iPSCs/ESCs and not differentiated SCs. Hence, the high density lectin microarray has proved to be valid for not only comprehensive analysis of glycans but also diagnosis of stem cells under the concept of the cellular glycome.

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“…They exhibit multipotency, that is, adipogenic, osteogenic, and chondrogenic differentiation in vitro [10]. They also show myogenic differentiation in vitro like mesenchymal stem cells derived from the bone marrow, menstrual blood, and placenta [6, 8, 13, 21–23]. These mesenchymal stromal cells can be used for therapeutic agents or delivery vehicles to patients with graft-versus-host disease, ischemic heart disease, and lysosomal storage disorders.…”

Section: Discussionmentioning

confidence: 99%

Can Human Embryonic Stem Cell-Derived Stromal Cells Serve a Starting Material for Myoblasts?

Ando

Saito

Machida

et al. 2017

Stem Cells International

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A large number of myocytes are necessary to treat intractable muscular disorders such as Duchenne muscular dystrophy with cell-based therapies. However, starting materials for cellular therapy products such as myoblasts, marrow stromal cells, menstrual blood-derived cells, and placenta-derived cells have a limited lifespan and cease to proliferate in vitro. From the viewpoints of manufacturing and quality control, cells with a long lifespan are more suitable as a starting material. In this study, we generated stromal cells for future myoblast therapy from a working cell bank of human embryonic stem cells (ESCs). The ESC-derived CD105+ cells with extensive in vitro proliferation capability exhibited myogenesis and genetic stability in vitro. These results imply that ESC-derived CD105+ cells are another cell source for myoblasts in cell-based therapy for patients with genetic muscular disorders. Since ESCs are immortal, mesenchymal stromal cells generated from ESCs can be manufactured at a large scale in one lot for pharmaceutical purposes.

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Cells of extraembryonic mesodermal origin confer human dystrophin in the Mdx model of duchenne muscular dystrophy

Cited by 27 publications

References 21 publications

Feasibility of placenta-derived mesenchymal stem cells as a tool for studying pregnancy-related disorders

Feasibility of placenta-derived mesenchymal stem cells as a tool for studying pregnancy-related disorders

Glycome Diagnosis of Human Induced Pluripotent Stem Cells Using Lectin Microarray

Can Human Embryonic Stem Cell-Derived Stromal Cells Serve a Starting Material for Myoblasts?

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