Cells producing insulin-like androgenic gland hormone of the giant freshwater prawn, Macrobrachium rosenbergii, proliferate following bilateral eyestalk-ablation

Phoungpetchara, Ittipon; Tinikul, Yotsawan; Poljaroen, Jaruwan; Chotwiwatthanakun, Charoonroj; Vanichviriyakit, Rapeepun; Sroyraya, Morakot; Hanna, Peter; Sobhon, Prasert

doi:10.1016/j.tice.2011.02.001

Cited by 25 publications

(19 citation statements)

References 26 publications

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“…In this respect, extensive AG hypertrophy and hyperplasia upon endocrine manipulation (elimination of this negatively regulating tissue) has previously been documented (Khalaila et al, 2002;Ventura et al, 2009). This general hypertrophy was further described in other species, as characterized by changes in the distribution of certain AG cell types (Phoungpetchara et al, 2011), increased numbers of IAG hormone-producing cells (Sroyraya et al, 2010), and IAG transcript overexpression (Chung et al, 2011). The induced overexpression of Cq-MAG and Cq-IAG under XO-SG deprivation provides important evidence for the specific negative control of neuroendocrine factors over the expression profile of AG activity-related factors in this crayfish.…”

Section: Discussionsupporting

confidence: 57%

An androgenic gland membrane-anchored gene associated with the crustacean insulin-like androgenic gland hormone

Rosen

Manor

Weil

et al. 2013

Journal of Experimental Biology

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SUMMARYCrustacean male sexual differentiation is governed by the androgenic gland (AG) and specifically by the secreted insulin-like AG hormone (IAG), thus far identified in several decapod species including the Australian red claw crayfish Cherax quadricarinatus (termed Cq-IAG). While a few insulin-like AG genes have been identified in crustaceans, other AG-specific genes have not been documented until now. In the present study, we describe the recent identification of a non-IAG AG-specific transcript obtained from the C. quadricarinatus AG cDNA library. This transcript, termed C. quadricarinatus membrane-anchored AG-specific factor (Cq-MAG), was fully sequenced and found to encode a putative product of 189 amino acids including a signal anchoring peptide. Expression of a recombinant GFP fusion protein lacking the signal anchor encoding sequence dramatically affected recombinant protein localization pattern. While the expression of the deleterious fusion protein was observed throughout most of the cell, the native GFP::Cq-MAG fusion protein was observed mainly surrounding the periphery of the nucleus, demonstrating an endoplasmic reticulum (ER)-like localization pattern. Moreover, co-expression of the wild-type Cq-MAG (fused to GFP) and the Cq-IAG hormone revealed that these peptides indeed co-localize. This study is the first to report a protein specifically associated with the insulin-like AG hormone in addition to the finding of another AG-specific transcript in crustaceans. Previous knowledge suggests that insulin/insulin-like factor secretion involves tissue-specific transcripts and membrane-anchored proteins. In this regard, Cq-MAGʼs tissue specificity, anchoring properties and intracellular co-localization with Cq-IAG suggest that it may play a role in the processing and secretion of this insulin-like AG hormone.

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Section: Discussionsupporting

confidence: 57%

An androgenic gland membrane-anchored gene associated with the crustacean insulin-like androgenic gland hormone

Rosen

Manor

Weil

et al. 2013

Journal of Experimental Biology

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“…Sroyraya et al (2010) observed that the AG of bilateral eyestalk ablated P. pelagicus underwent hypertrophy with a two-fold increase in the percentage of type I cells compared to the unablated controls. Phoungpetchara et al (2011) observed AG hypertrophy with proliferation and predominance of type I cells in M. rosenbergii subjected to bilateral eyestalk ablation. Vázquez-Islas et al 2015recorded a significant rise in the mean cell and nuclear diameters of AG in -E2 intermoult L. vannamei.…”

Section: Discussionmentioning

confidence: 83%

“…Investigations of Kulkarni et al (1984) suggested that eyestalk hormones have an inhibitory influence on secretory activity of the AG P. hardwickii. Studies of Phoungpetchara et al (2011) suggested that sinus glands in the eyestalk had some factors that inhibit the activity of the AG, GIH being the most important one. In vitro investigations showed direct inhibition of the AG by sinus gland extracts in C. quadricarinatus (Khalaila et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Kim et al (2002) observed the effects of eyestalk ablation on protein synthesis of AG and gonad development in reproductive and non-reproductive adult males of M. nipponense. Phoungpetchara et al (2011) and Revathi et al (2013) observed hypertrophied AG in bilaterally destalked M. rosenbergii. The present attempt has been made to evaluate the impact of unilateral and bilateral eyestalk ablation during active and revival phases of the AG in the freshwater crab T. schirnerae.…”

Section: Introductionmentioning

confidence: 92%

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Impact of eyestalk ablation on androgenic gland activity in the freshwater crab Travancoriana schirnerae Bott, 1969 (Decapoda: Gecarcinucidae)

Padmanabhan¹,

Raghavan²

2016

Braz. J. Biol. Sci.

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The current study investigated the impact of eyestalk ablation on androgenic gland activity in the freshwater crab Travancoriana schirnerae Bott, 1969 (Decapoda: Gecarcinucidae). Bilateral eyestalk ablation during active phase (March-June) induced hypertrophy and hyperplasia of the gland as evidenced by increased width of the gland, thickness of the connective tissue layer, increase in cell and nuclear diameter with predominance of type I cells. This hypertrophy and hyperplasia was not followed by secretory activity during active phase which possibly suggests the non-functional nature of hypertrophy and hyperplasia. Unilateral destalkation during active phase neither caused hypertrophy nor hyperplasia of the gland cells probably because these crabs may still receive the inhibitory principle from the remaining intact eyestalk causing the gland to remain analogous to that of the unablated controls. The androgenic gland of control crabs during revival phase (January-February) was discerned with a few patches of proliferated type I cells scattered among vacuoles and degenerating cells. The most prominent feature of the gland of bilaterally ablated crabs during revival phase was the extensive proliferation of type I cells. Not much significant change was observed in the morphology and histology of the gland of unilaterally ablated crabs of revival phase.

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“…To date, full‐length homologous IAG cDNA have been cloned in many commercially important decapod crustaceans, including crabs (Chung, Manor, & Sagi, ; Huang et al, ; Lawrence et al, ), crayfish (Levy et al, ; Manor et al, ; Rosen et al, ), prawns (Banzai et al, ; Ma et al, ; Phoungpetchara et al, ), and other shrimps (Li, Li, Sun, & Xiang, ; Vazquez‐Islas, Garza‐Torres, Guerrero‐Tortolero, & Campos‐Ramos, ; Zhang, Sun, & Liu, ). However, multiple sequence alignment showed that these IAG shared low similarities among different genus.…”

Section: Discussionmentioning

confidence: 99%

Identification of an insulin‐like androgenic gland hormone transcript highly expressed in male precociousness of the Chinese mitten crab Eriocheir sinensis

2018

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The androgenic gland (AG) hormone is well known as a key peptide to induce masculinization and maintains male characteristic in crustaceans. In this study, a fulllength cDNA encoding insulin-like androgenic gland hormone (EsIAG) was identified from the AG of the Chinese mitten crab Eriocheir sinensis. The full length of EsIAG cDNA contains 1,481 nucleotides, which encodes for a deduced protein of 151 amino acid residues. The predicted pre-peptide is composed of a signal peptide of 19 amino acids (aa), A chain of 44 aa, B chain of 31 aa, and C peptide of 57 aa. Moreover, six conserved cysteine residues and a putative N-linked glycosylated site were found in the deduced protein as reported in other crustaceans. Reverse transcription polymerase chain reaction and in situ hybridization analysis showed EsIAG was only expressed in the AG with especially high abundance during the mature stage of testis. Interestingly, a significantly higher expression level of EsIAG was also detected in precocious individuals than that in the normal grown ones, suggesting that the abnormally high expression of EsIAG might contribute to male precociousness in E. sinensis. K E Y W O R D SEriocheir sinensis, insulin-like androgenic gland hormone, precociousness

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Cells producing insulin-like androgenic gland hormone of the giant freshwater prawn, Macrobrachium rosenbergii, proliferate following bilateral eyestalk-ablation

Cited by 25 publications

References 26 publications

An androgenic gland membrane-anchored gene associated with the crustacean insulin-like androgenic gland hormone

An androgenic gland membrane-anchored gene associated with the crustacean insulin-like androgenic gland hormone

Impact of eyestalk ablation on androgenic gland activity in the freshwater crab Travancoriana schirnerae Bott, 1969 (Decapoda: Gecarcinucidae)

Identification of an insulin‐like androgenic gland hormone transcript highly expressed in male precociousness of the Chinese mitten crab Eriocheir sinensis

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