Cells synchronized in S phase show increased rate of repair of UV damaged plasmids

Gospodinov, Anastas; Anachkova, Boyka

doi:10.1016/j.febslet.2004.07.015

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…In mammalian cells the situation is more complex, because NER acts also in S phase, where GG-NER is enhanced [88] and is stimulated by active ATR [89,90]. Indeed, ATR-deficient Seckel syndrome fibroblasts exhibit attenuation of S phase specific GG-NER and a similar effect has been detected in XPV skin fibroblasts, deficient in pol η [91].…”

Section: Replicating Uv Damaged Dnamentioning

confidence: 99%

Mind the gap: Keeping UV lesions in check

et al. 2011

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Cells respond to genotoxic insults by triggering a DNA damage checkpoint surveillance mechanism and by activating repair pathways. Recent findings indicate that the two processes are more related than originally thought. Here we discuss the mechanisms involved in responding to UV-induced lesions in different phases of the cell cycle and summarize the most recent data in a model where Nucleotide Excision Repair (NER) and exonucleolytic activities act in sequence leading to checkpoint activation in non replicating cells. The critical trigger is likely represented by problematic intermediates that cannot be completely or efficiently repaired by NER. In S phase cells, on the other hand, the replicative polymerases, blocked by bulky UV lesions, re-initiate DNA synthesis downstream of the lesions, leaving behind a ssDNA tract. If these gaps are not rapidly refilled, checkpoint kinases will be activated.

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Section: Replicating Uv Damaged Dnamentioning

confidence: 99%

Mind the gap: Keeping UV lesions in check

et al. 2011

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“…The former may be pre y straigh orward -crea ng an bodies specific to the damaged molecular species, labelling the an bodies with a readily detectable label, and monitoring the occurrence and the distribu on of the signal. For example, monoclonal an bodies have been created against virtually all types of modified bases and photoproducts in DNA [388,389]. An bodies labelled with fluorescent reporter molecules (e.g.…”

Section: Pulsed Field Electrophoresismentioning

confidence: 99%

Individual capacity for detoxification of genotoxic compounds and repair of DNA damage. Commonly used methods for assessment of capacity for DNA repair

Chakarov¹,

Petkova²,

Russev³

2014

Biodiscovery

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The first part of this paper reviews the major achievements in the rapidly expanding field of research of individual capacity for repair of genotoxic damage. The issues of individual repair capacity are addressed from mul ple sides, analyzing the impact of the heritable components of the capacity for detoxifica on of genotoxic compounds, on the one hand (determining the risk for occurrence of DNA damage) and of the capacity for repair of DNA damage (when it has already occurred), on the other hand. The role of the capacity for repair of damage to DNA is discussed in the cons tu on of the risk for development of disease (mainly cancer, but also other common diseases and condi ons, such as diabetes, atherosclerosis and cardiovascular disease) and as a major factor in the outcomes of genotoxic therapies (eligibility for therapy with specific agents, risk for severe adverse effects, post-therapeu c survival rates, etc.). The paper contains an extensive list of biomarkers (mainly DNA polymorphisms, but also enzymes and other phenotypic markers, such as markers of the capacity for self-renewal of cell popula ons) that may be poten ally applicable in the assessment of the risk for carcinogenesis or for development other types of human disease.The second part of the paper provides a brief glimpse of the basic methodology used to obtain experimental results in assessment of the efficiency of DNA repair in living cells for research and diagnos c purposes.Cita on: Chakarov S, Petkova R, Russev GCh. Individual capacity for detoxifica on of genotoxic compounds and repair of DNA damage. Commonly used methods for assessment of capacity for DNA repair.

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Influence of Interferons on the Repair of UV-Damaged DNA

Tsoncheva

Todorovа

Ivanov

et al. 2008

Zeitschrift Für Naturforschung C

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The capacity for nucleotide excision repair of a normal (WISH) and three tumour (MCF-7, HeLa, Namalva) cell lines treated with human recombinant interferons (hrIFN-α and hrIFN-γ) was compared by the host cell reactivation assay. The cells were transfected with in vitro UV-damaged plasmid DNA (pEGFP-N1). The repair capacity was determined by measuring the fluorescence intensity of the expressed marker protein in total cell lysates. The correlation between the interferon-induced NO content and the suppressive effect of interferons on DNA repair was shown. The decrease of repair activity and NO induction by hrIFN-α were greatest in WISH, followed by MCF-7, Namalva and HeLa cells, whereas hrIFN-γ was the best NO inducer and inhibitor for the repair of Namalva, followed by WISH, MCF-7 and HeLa cells. Our data clearly show that the two types of interferon have a strong inhibitory effect on the repair of UV-damaged DNA and this effect is cell type-dependent

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Cells synchronized in S phase show increased rate of repair of UV damaged plasmids

Cited by 3 publications

References 26 publications

Mind the gap: Keeping UV lesions in check

Mind the gap: Keeping UV lesions in check

Individual capacity for detoxification of genotoxic compounds and repair of DNA damage. Commonly used methods for assessment of capacity for DNA repair

Influence of Interferons on the Repair of UV-Damaged DNA

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