Cellular Activation and Intracellular HCV Load in Peripheral Blood Monocytes Isolated from HCV Monoinfected and HIV-HCV Coinfected Patients

Dichamp, Isabelle; Abbas, Wasim; Kumar, Amit; Martino, Vincent Di; Herbein, Georges

doi:10.1371/journal.pone.0096907

Cited by 6 publications

(5 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the current study, the PBMCs RNA was found to be highest among the highly positive (45.4%) patient group as compared with the low-positive patient group. This shows that the patients having high viral load at the start of treatment were at a greater risk of viral relapse as compared with patients having low titer of HCV-RNA, which is in conformity with previously reported studies (8,12). Immunocytochemical staining technique was previously reported by our group for the detection of nonstructural proteins in PBMCs of local population (31).…”

Section: Discussionsupporting

confidence: 91%

The Absence of HCV RNA and NS5A Protein in Peripheral Blood Mononuclear Cells Is a Prognostic Tool for Sustained Virological Response

et al. 2017

View full text Add to dashboard Cite

Hepatitis C Virus (HCV) infection is a major health concern worldwide. The presence of both HCV viral RNA and NS5A proteins in peripheral blood mononuclear cells (PBMCs) indicate the efficacy of the treatment during sustained virological response (SVR) and end of treatment response (ETR). The main objective of this study was to detect the absence or presence of HCV RNA and NS5A proteins in PBMCs. Blood samples were taken from selected patients (Islamabad, Pakistan) before treatment, at ETR, and during SVR. Two hundred HCV responders to pegylated IFN-α-2a plus ribavirin were selected. HCV RNA was extracted from the patients to determine the viral load by reverse transcription (RT)-polymerase chain reaction before treatment. Out of 200 patients, 152 (76%) and 48 (24%) achieved positive and negative ETR, respectively. Among ETR patients, 134 (88.2%) showed SVR, whereas 18 (11.8%) displayed relapse. The male to female ratio was 92:108 with mean age of 37.4 years. Among 152 ETR-positive patients, 29 (19%) patients' PBMCs were positive for HCV RNA and 27 (17.8%) were positive for NS55A proteins. Patients having HCV RNA in PBMCs showed higher relapse frequency compared with patients lacking it. Similarly, patients having NS5A protein showed significantly higher relapse frequency compared with patients lacking it. All PBMC-positive samples were of genotype 3a. In addition, patients with positive NS5A in their PBMCs showed greater risk of relapse compared with patients having HCV RNA. We conclude that the absence of both viral HCV and proteins can be used as an indicator for diagnosis of SVR in the future.

show abstract

Section: Discussionsupporting

confidence: 91%

The Absence of HCV RNA and NS5A Protein in Peripheral Blood Mononuclear Cells Is a Prognostic Tool for Sustained Virological Response

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In another instance, role of CCL-2 has been shown in enhancing the replication of HIV-1 in PBMCs isolated from patients [ 58 ]. These monocyte subsets (CD14 ++ CD16 + and CD14 + CD16 ++ ) have been also reported in HCV infection demonstrating that CD16 + monocytes may play important role in viral diseases [ 59 , 60 ].…”

Section: Hiv-1 Infection Of Monocytes/macrophagesmentioning

confidence: 88%

Eradication of HIV-1 from the Macrophage Reservoir: An Uncertain Goal?

Abbas

Tariq

Iqbal

et al. 2015

Viruses

Self Cite

View full text Add to dashboard Cite

Human immunodeficiency virus type 1 (HIV-1) establishes latency in resting memory CD4+ T cells and cells of myeloid lineage. In contrast to the T cells, cells of myeloid lineage are resistant to the HIV-1 induced cytopathic effect. Cells of myeloid lineage including macrophages are present in anatomical sanctuaries making them a difficult drug target. In addition, the long life span of macrophages as compared to the CD4+ T cells make them important viral reservoirs in infected individuals especially in the late stage of viral infection where CD4+ T cells are largely depleted. In the past decade, HIV-1 persistence in resting CD4+ T cells has gained considerable attention. It is currently believed that rebound viremia following cessation of combination anti-retroviral therapy (cART) originates from this source. However, the clinical relevance of this reservoir has been questioned. It is suggested that the resting CD4+ T cells are only one source of residual viremia and other viral reservoirs such as tissue macrophages should be seriously considered. In the present review we will discuss how macrophages contribute to the development of long-lived latent reservoirs and how macrophages can be used as a therapeutic target in eradicating latent reservoir.

show abstract

“…Owing to these viruses sharing similar transmission routes, coinfections are common, especially among persons who inject drugs (PWID) (7,30,34). These viruses affect the immune system by increasing immune activation and causing changes to T cell subset distribution (7,8,12,14,27). Our previous research (14) and that of others (5,7,9,11,27) have shown the importance of CD4…”

mentioning

confidence: 99%

T Cell Distribution in Relation to HIV/HBV/HCV Coinfections and Intravenous Drug Use

et al. 2016

View full text Add to dashboard Cite

Intravenous drug use (IDU) is one of the most important transmission routes for blood borne viruses, including human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV). These infections alter the subset distributions of T cells; however, knowledge of such effects during HIV, HBV, and or HCV coinfection is limited. Therefore, we aimed to evaluate any associations between T cell distribution and the presence of HIV, HBV, and HCV coinfections among persons who inject drugs (PWID). Blood samples from 88 Caucasian PWID (mean age 30; 82% male) and 47 age-matched subjects negative for all three infections (mean age of 29; 83% male) were analyzed. The T cell markers CD3, CD4, CD8, CD45RA, CCR7, HLA-DR, and CCR5 were assessed using flow cytometry. Of the PWID, 40% were HIV+HBV+HCV+, 20% HBV+HCV+, 19% HCV+, and 13% negative for all three infections. The HIV+HBV+HCV+ PWID had lower percentages of CD4 + and higher percentages of CD8 + cells compared to triple negative PWID ( p < 0.001 in all cases). The only difference between HBV+HCV+ with triple negative PWID was the lower CD4 + cell percentages among the former (52.1% and 58.6%, p = 0.021). Triple negative PWID had higher immune activation and number of CCR5+ cells compared to the controls. We suggest that the altered T cell subset distribution among PWID is mainly triggered by HIV infection and or IDU, while HBV and or HCV seropositivity has minimal additional effects on CD4 + cell distribution.

show abstract

Cellular Activation and Intracellular HCV Load in Peripheral Blood Monocytes Isolated from HCV Monoinfected and HIV-HCV Coinfected Patients

Cited by 6 publications

References 52 publications

The Absence of HCV RNA and NS5A Protein in Peripheral Blood Mononuclear Cells Is a Prognostic Tool for Sustained Virological Response

The Absence of HCV RNA and NS5A Protein in Peripheral Blood Mononuclear Cells Is a Prognostic Tool for Sustained Virological Response

Eradication of HIV-1 from the Macrophage Reservoir: An Uncertain Goal?

T Cell Distribution in Relation to HIV/HBV/HCV Coinfections and Intravenous Drug Use

Contact Info

Product

Resources

About