Cellular and molecular immune response and production performance of Indian white shrimp Penaeus indicus (H. Milne-Edwards, 1837), reared in a biofloc-based system with different protein levels of feed

Panigrahi, A.; Das, Rashmi Ranjan; Maruthamuthu, S.; Sivakumar, M.R.; Jannathulla, Rajabdeen; Lalramchhani, Christina; Antony, Jose; Anand, P.S. Shyne; Kumar, Kuldeep; Jayanthi, M.; Dayal, J. Syama

doi:10.1016/j.fsi.2021.09.002

Cited by 13 publications

(3 citation statements)

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“…(2005), increased PE expression is correlated with phagocytosis activity, which is one of the shrimp's main defence mechanisms against pathogen infections. Increased PE expression has also been reported as a result of rearing in a biofloc system with differences in protein feed levels; all protein feed levels in the biofloc system showed better PE expression compared to the control treatment (Panigrahi et al., 2021).…”

Section: Discussionmentioning

confidence: 90%

Unveiling the positive impact of biofloc culture on Vibrio parahaemolyticus infection of Pacific white shrimp by reducing quorum sensing and virulence gene expression and enhancing immunity

Widanarni,

Gustilatov,

Ekasari

et al. 2024

Journal of Fish Diseases

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This study aimed to evaluate and unveil the positive impact of biofloc culture on Vibrio parahaemolyticus infection of Pacific white shrimp by reducing quorum sensing (QS) and virulence gene expression and enhancing shrimp's immunity. The shrimp with an average body weight of 0.50 ± 0.09 g were reared in containers with a volume of 2.5 L, 21 units, and a density of 20 shrimp L−1. The shrimp were cultured for 5 days, with each treatment including biofloc system maintenance with a C/N ratio of 10 and a control treatment without biofloc, followed by a challenge test through immersion using V. parahaemolyticus at densities of 103, 105, and 107 CFU mL−1 initially. The results of the in vitro experiment showed that biofloc suspension can inhibit and disperse biofilm formation, as well as reduce the exo‐enzyme activity (amylase, protease, and chitinase) of V. parahaemolyticus. Furthermore, the biofloc treatment significantly reduced the expression of the QS regulatory gene OpaR, the PirB toxin gene, and the virulence factor genes T6SS1 and T6SS2 in both in vitro and in vivo. The biofloc system also increased the expression of shrimp immunity‐related genes (LGBP, proPO, SP, and PE) and the survival rate of white shrimp challenged with V. parahaemolyticus.

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Section: Discussionmentioning

confidence: 90%

Unveiling the positive impact of biofloc culture on Vibrio parahaemolyticus infection of Pacific white shrimp by reducing quorum sensing and virulence gene expression and enhancing immunity

Widanarni,

Gustilatov,

Ekasari

et al. 2024

Journal of Fish Diseases

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“…Recently, as a part of the indigenous penaeid revival, the Indian white shrimp was selected as a national priority species due to its growth and reproductive potential (Anand et al, 2019;Vijayan, 2019;Anand et al, 2023). The successful nursery and field trials recorded average productivity of 3-7 tons per ha based on stocking density and geographical conditions (CIBA, 2019;Christina et al, 2020;Anand et al, 2021;Panigrahi et al, 2021). Although sustainable production of native penaeids is greatly desired, penaeid shrimp seed-producing hatcheries still depend on wild broodstocks for native shrimp seed production.…”

Section: Introductionmentioning

confidence: 99%

Reproductive performance of captive-reared Indian white shrimp, Penaeus indicus, broodstocks over two generations

et al. 2023

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Closing the complex life cycle of closed thelycum shrimp in captivity is one of the fundamental challenges in breeding programs. In the present study, we investigated the sexual maturity, broodstock development, and spawning performance of two generations of captive-reared Indian white shrimp, Penaeus indicus, over 36 months originated from a single stock of wild brooders. The post larvae (GN-1) produced from wild P. indicus (G0) and P.L. (GN-2) produced from captive-reared (GN-1) broodstocks were nursery (1000 PL m-3) and grow-out (12 shrimp m-2) reared, and subsequently raised in broodstock ponds (1 shrimp m-2). The annual salinity and photoperiod in broodstock ponds varied between 20 to 36 ppt, and 11.2 -12.5 L and 11.5-12.8 D hours, respectively. The light intensity varied between 91 ± 6 lux at dusk to 75,358 ± 1719 lux at noon. The size at first impregnation or mating was 16.45 ± 1.7 g (132 DOC) and 17.62 ± 1.9 g (90 DOC), respectively, in GN-1 and GN-2 females. By 220 DOC, 25% of the GN-1 females initiated gonad development, whereas 55% of the GN-2 females recorded developing ovaries or were in stage II at 150 DOC. The broodstock attained an average final body weight of 38.85 ± 1.5 g (GN-1) and 42.65 ± 1.8 g (GN-2) by 360 DOC. The highest (p<0.01) eggs per gram body weight (5137 ± 303 eggs g-1) and hatchability, H (83 ± 0.7%), was recorded in wild broodstocks (G0) followed by GN-2 (1,715 ± 162 eggs g-1; H: 69 ± 2%) and GN-1 (1,476 ± 151 eggs g-1; H: 75 ± 1%). However, captive-reared broodstock had better survival (89-92%) than wild broodstocks (71 ± 0.8%). Further, indoor maturation trial (21 days) using ablated broodstocks (GN-1) revealed 79% of the impregnated broodstocks undergo molting, resulting in the loss of sperm pack and subsequent reduction in mating efficacy to 29%. The average sperm count and percentage of normal sperm also recorded (p<0.05) reduction during the maturation cycle. The data generated in the present investigation can form the baseline information for developing the breeding strategy for the genetic improvement of Indian white shrimp in India.

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“…By converting the nitrogenous wastes into microbial biomass, biofloc technology (BFT) was applied in the aquaculture system to keep the water quality without a large-scale water change and the biofloc particles could also be taken by shrimps or fish as additional nutrition ( Santhana Kumar et al, 2018 ; Putra et al, 2020 ; Van Doan et al, 2021 ). In shrimp culture system, studies suggested that biofloc can enhance the growth and the immune response of shrimps by altering the bacterial communities and reusing the waste in the water ( Jamal et al, 2020 ; Addo et al, 2021 ; Panigrahi et al, 2021 ; Xu et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Screening of bioflocculant and cellulase-producing bacteria strains for biofloc culture systems with fiber-rich carbon source

Chen²,

Xu³

et al. 2022

Front. Microbiol.

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The biofloc technology (BFT) system has been widely applied in the shrimp and fish culture industry for its advantages in water-saving, growth improvement, and water quality purification. However, The BFT system usually takes a long time to establish, and the extra carbon source input increases the maintenance cost of the system. In this study, we aimed to develop a low-cost and high-efficient BFT system for Litopenaeus vannamei by applying bacteria that could promote the formation of BFT and utilize cheap carbon sources. Three bioflocculant-producing bacteria strains (M13, M15, and M17) have been screened from a cellulolytic strain collection. All three strains have been identified as Bacillus spp. and can use sugarcane bagasse (SB) as a carbon source, which is a cheap byproduct of the sucrose industry in the tropic area of China. Compared to sucrose, the addition of SB and the three strains could improve the biofloc formation rate, biofloc size distribution, ammonia removal rate, and the growth performance of the shrimps. These results suggest that the bioflocculant and cellulase-producing bacteria strains could promote the biofloc formation and the growth of shrimps by using SB as an economic substitute carbon source in the BFT shrimp culture system.

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Cellular and molecular immune response and production performance of Indian white shrimp Penaeus indicus (H. Milne-Edwards, 1837), reared in a biofloc-based system with different protein levels of feed

Cited by 13 publications

References 63 publications

Unveiling the positive impact of biofloc culture on Vibrio parahaemolyticus infection of Pacific white shrimp by reducing quorum sensing and virulence gene expression and enhancing immunity

Unveiling the positive impact of biofloc culture on Vibrio parahaemolyticus infection of Pacific white shrimp by reducing quorum sensing and virulence gene expression and enhancing immunity

Reproductive performance of captive-reared Indian white shrimp, Penaeus indicus, broodstocks over two generations

Screening of bioflocculant and cellulase-producing bacteria strains for biofloc culture systems with fiber-rich carbon source

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