Cellular differentiation, bioactive and mechanical properties of experimental light-curing pulp protection materials

Sauro, Salvatore; Babbar, Ashvin; Gharibi, Borzo; Feitosa, Victor Pinheiro; Carvalho, Ricardo Marins de; Rodrigues, Lidiany Karla Azevedo; Banerjee, Avijit; Watson, Timothy F.

doi:10.1016/j.dental.2018.02.008

Cited by 21 publications

(12 citation statements)

References 49 publications

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“…The presence of calcium (Ca) was found in the three tested GICs. The release of calcium hydroxide is important because it plays a role in odontoblastic differentiation and dentin bridge formation, and in addition to that because it is responsible for the antimicrobial activity of this type of bioactive material [3,37]. Also, we detected fluoride and aluminum in all tested materials.…”

Section: Discussionmentioning

confidence: 90%

“…The use of materials with remineralization effects is essential in deep caries with a significant risk of pulp exposure because, during carious tissue removal, bacterially contaminated and demineralized dentin is left in the pulp region and sealed beneath the restorative material, favoring remineralization of the sealed carious lesions through ion release [1,3].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Evaluation of the Biological Effects of ACTIVA Kids BioACTIVE Restorative, Ionolux, and Riva Light Cure on Human Dental Pulp Stem Cells

et al. 2019

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This study aimed to analyze the biological effects of three new bioactive materials on cell survival, migration, morphology, and attachment in vitro. ACTIVA Kids BioACTIVE Restorative (Pulpdent, Watertown, MA, USA) (Activa), Ionolux (Voco, Cuxhaven, Germany), and Riva Light Cure UV (SDI, Bayswater, Australia) (Riva) were handled and conditioned with a serum-free culture medium. Stem cells from human dental pulp (hDPSCs) were exposed to material extracts, and metabolic activity, cell migration, and cell morphology were evaluated. Cell adhesion to the different materials was analyzed by scanning electron microscopy (SEM). The chemical composition of the materials was evaluated by energy-dispersive X-ray (EDX). One-way analysis of variance followed by a Tukey test was performed (p < 0.05). Ionolux promoted a drastic reduction in metabolic activity and wound closure compared to the control (p < 0.05), whereas Activa induced adequate metabolic activity and cell migration. Moreover, SEM and immunofluorescence analysis showed abundant cells exposed to Activa. The materials showed different surface morphologies, and EDX spectra exhibited different peaks of C, O, Si, S, Ca, and F ions in glass ionomer cements. The results showed that Activa induced cell migration, cell attachment, and cell viability to a greater extent than Riva and Ionolux.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of the Biological Effects of ACTIVA Kids BioACTIVE Restorative, Ionolux, and Riva Light Cure on Human Dental Pulp Stem Cells

et al. 2019

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“…Generally, bioactive materials have deficient mechanical properties and they present handling difficulties for clinical use. 39 Focusing on an in vitro laboratory evaluation, this study evaluates the mechanical, physical, and biological properties of a commercial resin-based dental cement incorporated with two types of ceramic particles: HA and 45S5 BG.…”

mentioning

confidence: 99%

Effect of hydroxyapatite and 45S5 bioactive glass addition on a dental adhesive resin cement

Assad

Batista

Anami³

et al. 2020

Int J of Appl Glass Sci

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Owing to their adequate adhesive, esthetic, and mechanical properties resin-based cement has been widely used in dental practice to bond metallic, ceramic, or polymeric indirect restorations (crowns, veneers, inlays, onlays, or fixed partial dentures) in mineralized dental tissues. 1-3 Compared to conventional types of cement, like zinc phosphate and glass ionomer, resin-based cement has improved adhesive and mechanical properties and it is less soluble. 2-4 However, because of their potential cytotoxic effect on pulp-dentine complex 5-7 and the possible occurrence of postoperative hypersensitivity owing to the absence of hermetical sealing on the tooth/cement interface provoked by a

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“…Unreacted monomers may gradually accumulate to a toxic level, irreversibly affect cells’ defence mechanisms and lead to cell apoptosis (Sauro et al . 2018). One in vitro study comparing the cytotoxic effects of MTA angelus, Biodentine, TheraCal LC and IRM reported a significant decrease in the percentages of viable, nonapoptotic and non‐necrotic cells in the hDPSCs (Bortoluzzi et al .…”

Section: Discussionmentioning

confidence: 99%

Influence of resveratrol application with pulp‐capping materials on the genetic expression levels of stem cells

Celik

Yapar

Taghizadehghalehjoughi

et al. 2020

Int Endodontic J

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AimTo evaluate in a laboratory setting the response of human mesenchymal stem cells (MSCs) to pulp‐capping materials with and without resveratrol (RSV).MethodologyFive materials, Calcimol LC, Life, TheraCal LC, ProRoot MTA and Biodentine, were prepared according to the manufacturers’ instructions. Human MSCs were then exposed to these materials, with and without RSV, for 24 h (n = 8). Cell viability was evaluated using the MTT assay, and total cell death was quantified by annexin V‐FITC staining with flow cytometry. The expression levels of the IL‐8, IL‐10, HBD‐2 and BCL‐2 genes were investigated using real‐time polymerase chain reaction (RT‐PCR). Data obtained from MTT test were analysed using one‐way anova, and Tukey’s multiple‐comparison test. The paired Student t test was employed to compare the effects of materials on gene expression (significance level of 5%).ResultsThe group cell viabilities were Calcimol LC 53%, Life 43%, TheraCal LC 78%, ProRoot MTA 75% and Biodentine 78%. Calcimol LC and Life exhibited significant differences compared with the control groups (P < 0.05). The percentages of necrotic/late apoptotic cells associated with Calcimol LC and TheraCal LC were greater than in the other materials. However, when RSV was added to wells containing materials, cell viability increased to Calcimol LC 63%, Life 52%, TheraCal LC 82%, ProRoot MTA 91% and Biodentine 96%, and the percentages of early apoptotic and late apoptotic/necrotic cells decreased. Calcimol LC + RSV and Life + RSV differed significantly from the control group (P < 0.05). The expression of IL‐8 gene was high for all materials, ProRoot MTA caused significant overexpression, and the addition of RSV reduced the expression of IL‐8 in the Calcimol LC, TheraCal LC and ProRoot MTA groups and led to increased expression of IL‐10 in the Calcimol LC, Life and Biodentine groups. HBD‐2 and BCL‐2 exhibited increased expression in ProRoot MTA with RSV (P < 0.05).ConclusionsThe addition of RSV exerted a protective effect on MSCs and regulated the inflammatory process by altering the expression levels of pro‐ and anti‐inflammatory genes.

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Cellular differentiation, bioactive and mechanical properties of experimental light-curing pulp protection materials

Cited by 21 publications

References 49 publications

In Vitro Evaluation of the Biological Effects of ACTIVA Kids BioACTIVE Restorative, Ionolux, and Riva Light Cure on Human Dental Pulp Stem Cells

In Vitro Evaluation of the Biological Effects of ACTIVA Kids BioACTIVE Restorative, Ionolux, and Riva Light Cure on Human Dental Pulp Stem Cells

Effect of hydroxyapatite and 45S5 bioactive glass addition on a dental adhesive resin cement

Influence of resveratrol application with pulp‐capping materials on the genetic expression levels of stem cells

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