Cellular Functionality on Nanotubes of Ti-30Ta Alloy

Abstract

Recent studies have identified strong correlations between anodized metals and the production of highly biomimetic nanoscale topographies. These surfaces provide an interface of enhanced biocompatibility that exhibits a high degree of oxidation and surface energy. In this study, Human dermal fibroblasts (HDF, neonatal) were utilized to evaluate the biocompatibility of Ti-30Ta nanotubes after 1 day of culture. The anodization process was performed in an electrolyte solution containing HF (48%) and H2SO4 (98%) in the volumetric ratios 1:9 with the addition of 5% dimethyl sulfoxide (DMSO) at 35V for 40 min. Cellular analysis identified improved fibroblast functionality on the nanotube surface, showing increased elongation, and extracellular matrix production on the Ti-30Ta nanotubes. The results presented identify improved cellular interaction on Ti-30Ta nanotubes as compared to the control substrates. Thus, the formation of the nanotube on Ti30Ta alloy may have potential application as interface for implantable devices.

Cell investigation into the biocompatibility of adult human dermal fibroblasts with PCL nanofibers/TiO2 nanotubes on the surface of Ti–30Ta alloy for biomedical applications

“…The anodization process was performed according to our previous studies 27,30‐33 . Briefly, a dual electrode system with platinum and the Ti–30Ta alloy fixed 15 mm apart was used.…”

“…The anodization process was performed according to our previous studies. 27,[30][31][32][33] Briefly, a dual electrode system with platinum and the Ti-30Ta alloy fixed 15 mm apart was used. The electrolyte contained 0.2 M NH 4 F and glycerol and the voltage applied was 30 V for 6 hours.…”

Atividade antimicrobiana e corrosão do titânio e da liga Ti-30Ta

“…Then, the alloy was cold -worked by a rotary swaging process and solubilized at 950 ºC for 2 hours followed by water cooling. The bars were cut into discs of 10 mm in diameter and 3 mm in thickness (Capellato, Smith, Popat, & Alves Claro, 2015). The samples were separated into group 1-Ti cp and group 2-Ti-30Ta.…”

Fabrication and cellular interactions of nanoporous tantalum oxide

“…Wang et al observed that anodized tantalum surfaces possessing nanotubular oxide morphology promoted rabbit bone marrow mesenchymal stem cell adhesion and proliferation and in the meantime upregulated expression of osteogenic genes, including alkaline phosphatase, collagen-I, and osteocalcin (Wang et al, 2012). In addition, fibroblast cells were observed to spread more on anodized nanotubular Ti30Ta alloy and showed enhanced extracellular matrix production compared to nonanodized control samples (Capellato, Smith, Popat, & Alves Claro, 2014). In spite of the promising in vitro biological results obtained for nanotubular tantalum surfaces, no research was completed either on the biocompatibility or on the optimal size regime of the pores present on anodized nanoporous tantalum oxide surfaces.…”