Cellular immune response of ducks to duck hepatitis B virus infection

Vickery, Karen; Cossart, Yvonne E.; Dixon, Robert

doi:10.1002/(sici)1096-9071(199905)58:1<19::aid-jmv3>3.0.co;2-r

Cited by 9 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a previous study, we used lymphocyte proliferation assays to detect responses to DHBV surface and core antigens during infection. We found that response to surface antigen was more strongly associated with virus clearance than responses to core [14], which is in accordance with the data from the transgenic mouse system. As a first step towards development of a therapeutic vaccine strategy, which can be tested in a natural hepadnavirus infection, we have now used these assays to define the immunodominant peptides in the DHBV surface antigen.…”

Section: Introductionsupporting

confidence: 87%

Identification of T‐cell epitopes associated with immunity within the surface protein of duck hepatitis B virus

Welschinger

Cossart

Pouliopoulos

et al. 2006

Journal of Viral Hepatitis

View full text Add to dashboard Cite

Duck hepatitis is a convenient model of hepatitis B virus (HBV) infection, but the lack of immunological reagents hampers investigation of pathogenesis and vaccine development. The aim of this study was to define T-cell epitopes in the surface peptide recognized by vaccinated immune birds. Blastogenesis assays were used to test the proliferative response of spleen mononuclear cells to synthetic peptides spanning the pre-S/S region in 22 naïve and 13 immunized and challenged immune ducks. Roughly > or = 50% of the immune ducks responded to five immunodominant peptides eliciting a statistically greater proliferative response than in naïve birds. Fewer ducks responded to an additional six peptides. No statistically significant difference could be shown for the response to 11 peptides between the immune ducks and the naïve ducks. There was no clustering of the immunodominant peptides which were located throughout the surface antigen at sites of major swings in hydrophobicity. A number of peptides which induce lymphoblastogenesis in vaccinated immune ducks have been identified. Their role in spontaneous recovery from duck hepatitis B infection merits investigation.

show abstract

Section: Introductionsupporting

confidence: 87%

Identification of T‐cell epitopes associated with immunity within the surface protein of duck hepatitis B virus

Welschinger

Cossart

Pouliopoulos

et al. 2006

Journal of Viral Hepatitis

View full text Add to dashboard Cite

show abstract

“…Antigen-specific blastogenic assays and lymphocyte proliferation tests provoked by nonspecific mitogens have been used extensively to assess the immunocompetence of poultry (Baxi and Oberoi, 1999;Vickery et al, 1999). In this study, a high level of NH 3 decreased the proliferation of peripheral blood lymphocytes.…”

Section: Discussionmentioning

confidence: 74%

Ammonia concentration and relative humidity in poultry houses affect the immune response of broilers

Wei

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT.To investigate the effect of ammonia (NH 3 ) and humidity on the immune response of broilers, broilers were exposed to 30 or 70 mg/kg atmospheric NH 3 for 21 days. Additionally, birds were exposed to 35, 60, and 85% relative humidity (RH). The relative weights of lymphoid organs, serum total protein, serum globulin, serum albumin, serum lysozyme, proliferation index of peripheral blood lymphocytes, and splenic cytokine gene expression were determined. Exposure to 70 mg/kg NH 3 decreased the relative weight of the spleen during the experimental period, serum lysozyme concentration in the first and second weeks, and serum globulin concentration in the third week. The proliferation of peripheral blood lymphocytes was reduced. High levels of NH 3 caused increase in IL-1β gene expression in the experimental period and IL-4 gene expression in the first week. Birds exposed to 85% RH had lower thymus and bursa of Fabricius weights in the third week and serum lysozyme concentration in the first week; IL-1β and IL-4 expressions were higher in the second and third weeks and first and second weeks, respectively, than in birds exposed to 60% RH. IL-4 expression was lower during the first week, and IL-1β expression was higher during the second week with 35% RH than with 60% RH. In conclusion, high NH 3 level in the poultry house suppressed the immune response of broiler chickens. Neither high nor low RH benefited the immune response of broilers. Furthermore, there was an interactive effect between NH 3 and RH on the immune response of broilers.

show abstract

“…Hence, the diagnostic assays based on serology are of not much significance, as well as are not useful in acute infection, serological surveillance and while measuring post-vaccination immune response (Toth 1972;Toth & Norcross 1981;Vickery et al 1999a, Vickery et al 1999a, 1999bTang et al 2001;Qi, Yang, Cheng, Wang, Zhu, et al 2009;Tohidi-Esfahani et al 2010;Yang et al 2010). Although cell-mediated immune response has been shown to play a more important role during DEV infection (Vickery et al 1999a(Vickery et al , 1999b, it is possible to detect the anti-DEV neutralizing antibodies (Saade et al 2008;Aravind et al 2012).…”

Section: Immunological / Serological Testsmentioning

confidence: 99%

Duck virus enteritis (duck plague) – a comprehensive update

Dhama

Kumar

Saminathan

et al. 2017

Veterinary Quarterly

View full text Add to dashboard Cite

Duck virus enteritis (DVE), also called duck plague, is one of the major contagious and fatal diseases of ducks, geese and swan. It is caused by duck enteritis virus (DEV)/Anatid herpesvirus-1 of the genus Mardivirus, family Herpesviridae, and subfamily Alpha-herpesvirinae. Of note, DVE has worldwide distribution, wherein migratory waterfowl plays a crucial role in its transmission within and between continents. Furthermore, horizontal and/ or vertical transmission plays a significant role in disease spread through oral-fecal discharges. Either of sexes from varying age groups of ducks is vulnerable to DVE. The disease is characterized by sudden death, vascular damage and subsequent internal hemorrhage, lesions in lymphoid organs, digestive mucosal eruptions, severe diarrhea and degenerative lesions in parenchymatous organs. Huge economic losses are connected with acute nature of the disease, increased morbidity and mortality (5%-100%), condemnations of carcasses, decreased egg production and hatchability. Although clinical manifestations and histopathology can provide preliminary diagnosis, the confirmatory diagnosis involves virus isolation and detection using serological and molecular tests. For prophylaxis, both live-attenuated and killed vaccines are being used in broiler and breeder ducks above 2 weeks of age. Since DEV is capable of becoming latent as well as shed intermittently, recombinant subunit and DNA vaccines either alone or in combination (polyvalent) are being targeted for its benign prevention. This review describes DEV, epidemiology, transmission, the disease (DVE), pathogenesis, and advances in diagnosis, vaccination and antiviral agents/therapies along with appropriate prevention and control strategies.

show abstract

Cellular immune response of ducks to duck hepatitis B virus infection

Cited by 9 publications

References 29 publications

Identification of T‐cell epitopes associated with immunity within the surface protein of duck hepatitis B virus

Identification of T‐cell epitopes associated with immunity within the surface protein of duck hepatitis B virus

Ammonia concentration and relative humidity in poultry houses affect the immune response of broilers

Duck virus enteritis (duck plague) – a comprehensive update

Contact Info

Product

Resources

About