Cellular immune responses in cetaceans immunized with a porcine erysipelas vaccine

Sitt, Tatjana; Bowen, Lizabeth; Blanchard, M; Gershwin, Laurel J.; Byrne, Barbara A.; Dold, Christopher; McBain, Jim; Stott, Jeffrey L

doi:10.1016/j.vetimm.2010.05.003

Cited by 23 publications

(26 citation statements)

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“…Signs of septicemic disease in infected cetaceans are usually of nonspecific, and death within a few hours of clinical presentation is not unusual (Dierauf and Gulland 2001;Sitt et al 2010). The dermatologic form of the disease occurs periodically and is typically accompanied by a leukocytosis and anorexia with subsequent development of skin lesions, the latter being most commonly visible on the trunk (Sitt et al 2010). After that, septicemia and death could follow within days or weeks if the animal is not treated (Wang et al 2010).…”

Section: Resultsmentioning

confidence: 99%

“…Regarding the value of implementing a vaccination program for erysipelas, it is important to mention that this fact became apparent after observing that the majority of reported clinical cases were related to animals that had not been vaccinated (Dierauf and Gulland 2001;Sitt et al 2010). Even though there is a wide variety of porcine erysipelas vaccines, only around 1/3 of them have been demonstrated to be effective in cetaceans (Dierauf and Gulland, 2001).…”

Section: Resultsmentioning

confidence: 99%

“…Even though there is a wide variety of porcine erysipelas vaccines, only around 1/3 of them have been demonstrated to be effective in cetaceans (Dierauf and Gulland, 2001). Moreover, most of the tested vaccines have shown adverse clinical reactions and even death of individuals (Howard et al 1983;Dierauf and Gulland 2001;Sitt et al 2010).…”

Section: Resultsmentioning

confidence: 99%

“…Most of the available vaccines in the market are composed of attenuated E. rhusiopathiae strains or bacterins and are effective in pigs and turkeys between 6 and 12 months (Groschup and Timoney 1990;Wood 1992;Swan and Lindsey 1998;Wang et al 2010). Regarding vaccination in dolphins, porcine erysipelas vaccines have been tested with successful results (Lacave et al 2001;Sitt et al 2010). …”

Section: Introductionmentioning

confidence: 99%

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Development of an indirect immunofluorescence technique for the evaluation of generated antibody titers against Erysipelothrix rhusiopathiae in captive bottlenose dolphins (Tursiops truncatus)

et al. 2014

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Erysipelothrix rhusiopathiae is the causative agent of erysipelas, a disease of many mammalian and avian species, mainly swine and turkeys. In cetaceans, erysipelas is considered to be the most common infection in juvenile individuals, which have not been vaccinated. Moreover, the disease manifest in both forms, the dermatologic and the acute septicemic forms, has been reported in various species of dolphins and whales. It is difficult to diagnose erysipelas by currently available approaches. Moreover, it is mainly based on culture methods and also PCR methods, which are currently being developed. At the present stage, prophylactic approaches are based on antibiotic therapy and vaccination mostly with porcine erysipelas vaccines. In the present study, an Indirect Immuno Fluorescence method for the detection of dolphin antibodies levels against E. rhusiopathiae was developed and applied in two different groups of captive bottlenose dolphins (Tursiops truncatus) from Loro Parque (Tenerife, Canary Islands, Spain) and L'Oceanogràfic de Valencia (Valencia, Spain) in order to check the tittering levels of antibodies after application of porcine erysipelas vaccines in the studied dolphins.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of an indirect immunofluorescence technique for the evaluation of generated antibody titers against Erysipelothrix rhusiopathiae in captive bottlenose dolphins (Tursiops truncatus)

et al. 2014

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“…Determination of antibodies against E. rhusiopathiae in captive cetaceans allows for the assessment of vaccine efficiency (Lacave et al 2001, Sitt et al 2010, Bernal-Guadarrama et al 2014. Its determination in unvaccinated captive and in free-ranging cetaceans may also allow detection of previous exposure and may assist in diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent microbead-based immunoassay for anti-Erysipelothrix rhusiopathiae antibody detection in cetaceans

Melero

Giménez-Lirola²,

Rubio-Guerri³

et al. 2016

Dis. Aquat. Org.

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A fluorescent microbead-based immunoassay (FMIA) for detection of antiErysipelothrix rhusiopathiae antibodies in pigs was adapted for use in cetaceans. The FMIA was validated and adjusted using serum samples from 10 vaccinated captive bottlenose dolphins Tursiops truncatus collected between 1 and 13 mo after immunization. The technique was then used to analyze specimens from 15 free-ranging cetaceans stranded alive on the Valencian Mediterranean coast between 2006 and 2014: 11 striped dolphins Stenella coeruleoalba, 3 Risso's dolphins Grampus griseus and 1 bottlenose dolphin Tursiops truncatus. One of these wild animals was confirmed to have died from E. rhusiopathiae septicemia, but no anti-E. rhusiopathiae antibodies were detected in its serum, pericardial fluid or milk samples. Another free-ranging individual, which lacked any signs or lesions that might be indicative of E. rhusiopathiae infection, showed high fluorescence intensity similar to that measured in captive dolphins at 6−13 mo after vaccination. These results suggest that this animal underwent an E. rhusiopathiae infection several months before stranding. The findings in the present study suggest that FMIA can be useful for detecting anti-E. rhusiopathiae antibodies in cetaceans, and its application to free-ranging animals is particularly interesting because of the great value of these specimens. Furthermore, the FMIA can be multiplexed to allow the determination of up to 100 analytes per sample in a single well, thereby reducing the cost, time and sample volume needed.

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Selection of a reference gene for studies on lipid‐related aquatic adaptations of toothed whales (Grampus griseus)

Senevirathna

Yonezawa

Saka

et al. 2021

Ecology and Evolution

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The Grampus griseus (Risso's dolphin) is a toothed whale species in the order Cetacea that exhibits cosmopolitanism (Gaspari et al., 2007;Gaspari & Natoli, 2012). Grampus griseu is the only species in the genus Grampus, the fifth-largest member of the family Delphinidae (Baird, 2009), and part of the subfamily Globicephalinae. Species in this subfamily could be cryptic species by evolutionary imperative (Thompson et al., 2013). Even within the subfamily Globicephalinae, G. griseus display a 0.022 distance from other species in the

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Cellular immune responses in cetaceans immunized with a porcine erysipelas vaccine

Cited by 23 publications

References 11 publications

Development of an indirect immunofluorescence technique for the evaluation of generated antibody titers against Erysipelothrix rhusiopathiae in captive bottlenose dolphins (Tursiops truncatus)

Development of an indirect immunofluorescence technique for the evaluation of generated antibody titers against Erysipelothrix rhusiopathiae in captive bottlenose dolphins (Tursiops truncatus)

Fluorescent microbead-based immunoassay for anti-Erysipelothrix rhusiopathiae antibody detection in cetaceans

Selection of a reference gene for studies on lipid‐related aquatic adaptations of toothed whales (Grampus griseus)

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