Cellular localisation of human epidermal growth factor receptor

Abstract

We show here, using immunohistological techniques and a monoclonal antibody to the receptor for epidermal growth factor (EGFR) (Waterfield et al. 1982) that EGFR is present on a wide range of normal epithelial tissues and tumours arising from those sites. The distribution of the receptor suggests that EGF may be involved in the control of proliferation and possibly differentiation of surface epithelia. The strong tumour cell staining suggests an increased expression of the receptor in certain carcinomas.

Epidermal growth factor receptor expression by human squamous cell carcinomas of the head and neck, cell lines and xenografts

“…Thus, it is not esssential for all SCCHN-derived cell lines to overexpress EGFR, nor is HPV16 E7 and E6 expression sufficient to cause more than a 2-fold increase in EGFR expression. (Waterfield et al, 1982), to cryosections of tumour biopsy specimens (Gusterson et al, 1984;Hendler & Ozanne, 1984;Ozanne et al, 1986;Hendier et al, 1989). To demonstrate the specificity of EGFR1, we observed the binding of EGF (Figure la and b) and EGFR1…”

HER-2 - A Possible Target for Therapy of Metastatic Urinary Bladder Carcinoma

“…U nfortunately, for targeted therapy, the EG F Rs are distributed among various normal tissues as well as tumours (14,15). The expression pattern of EG F R in metastatic bladder carcinoma has already been described by our group (16).…”

A sensitive enzyme-linked immunosorbent assay used for quantitation of epidermal growth factor receptor protein in head and neck carcinomas: evaluation, interpretations and limitations

“…Transforming growth factor alpha (TGF-x), amphiregulin (AR), vaccinia virus growth factor (VVGF), heparin-binding EGF-like growth factor (HB-EGF) and betacellulin bind to the EGFR which is present on cells derived from all three germ layers, including the proliferative compartment of epithelia (De Larco and Todaro, 1978;Gusterson et al, 1984;Nanney et al, 1984;Stroobant et al, 1985;Shoyab et al, 1989;Higashiyama et al, 1991;Sasada et al, 1993). The biological activities are initiated through a tyrosine kinase which is localised to the intracellular domain of EGFR (Chen et al, 1987).…”