Cellular origin, hormonal regulation and biochemical characteristics of polypeptides secreted by Graafian follicles of sheep

Moor, R. M.; Crosby, I. M.

doi:10.1530/jrf.0.0790469

Cited by 15 publications

(10 citation statements)

References 18 publications

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“…The 56 kDa (B) peak was secreted the most within the studied peaks. A stimulating effect of gonadotropins on the synthesis of proteins by granulosa cells has been reported previously (rats: Landefelt et al, 1979;cattle: Skinner and Osteen, 1988; sheep: Moor and Crosby, 1987). These changes occurred during the early steps of in vivo luteinization, which happens 20 hr after the LH surge in bovine (Dieleman and Blankenstein, 1985) and could therefore be involved either in ovulation or in oocyte maturation.…”

Section: Discussionmentioning

confidence: 62%

“…The conditions for labeling were examined to determine the incubation time and the amount of labeled precursor required for detecting the minimum quantity of radioactivity incorporated. Because the incorporation of 3H-methionine was time and dose dependent, a 3 h r incubation period was chosen (Moor and Crosby, 1987). Granulosa and cumulus cells for each follicle were incubated separately at 37°C with PBS-BSA containing 0.5 pM (40 pCi/ml) and 7.6 pM (625 pCi/ml) 3H-methionine, respectively.…”

Section: Incubation Conditionsmentioning

confidence: 99%

“…In control experiments, cycloheximide was added at 100 or 200 pM to inhibit protein synthesis. To check the results obtained by fluorography, granulosa and cumulus cells of individual follicles were incubated sepa-rately with 35S-methionine at 0.1 FM (100 pCi/ml) (Moor and Crosby, 1987) using the same conditions as described above.…”

Section: Incubation Conditionsmentioning

confidence: 99%

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Qualitative and quantitative changes in protein sythesis of bovine follicular cells during the preovulatory period

Rabahi

Monniaux

Pisselet

et al. 1991

Molecular Reproduction Devel

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To understand the mechanisms governing oocyte maturation better, the effects of the gonadotropin surge were studied on follicular cells of bovine preovulatory follicles. For this purpose, qualitative and quantitative changes in protein synthesis by both granulosa cells and cumulus cells were compared relative to the luteinizing hormone (LH) surge and the resumption of meiosis in the oocyte. Follicular cells were collected at different times before and up to 25 hr after the LH surge. For each individual preovulatory follicle, granulosa and cumulus cells were incubated separately for 3 hr with 3H-methionine or with 35S-methionine. Newly synthesized cytosolic proteins from granulosa and cumulus cells and proteins secreted into the medium were analyzed by polyacrylamide gel electrophoresis. The radioactivity was measured by liquid scintillation counting after slicing of the gels or revealed by fluorography. Three major peaks of the newly synthesized proteins, with molecular weights of 76, 56, and 30 kDa, were studied throughout the preovulatory period. After the LH surge, the overall level of protein synthesis increased in granulosa cells. In addition, the pattern of cytosolic proteins in granulosa cells changed, and, in particular, the relative synthesis of the 30 kDa peak decreased. These changes in cytosolic protein synthesis may be due to the action of LH since they could be reproduced in vitro in LH-stimulated granulosa cells. A predominant peak of 56 kDa was secreted by granulosa cells throughout the experimental period. No significant change was observed in proteins synthesized by cumulus cells under the same experimental conditions. The amounts of radioactivity incorporated into the three major proteins secreted by granulosa cells, however, were correlated significantly with the amounts of radioactivity incorporated by similar proteins synthesized by cumulus cells. These results indicate that cumulus cells respond differently from granulosa cells to the gonadotropin surge but not in an independent manner.

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Section: Discussionmentioning

confidence: 62%

Section: Incubation Conditionsmentioning

confidence: 99%

Section: Incubation Conditionsmentioning

confidence: 99%

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Qualitative and quantitative changes in protein sythesis of bovine follicular cells during the preovulatory period

Rabahi

Monniaux

Pisselet

et al. 1991

Molecular Reproduction Devel

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“…The junctional contact between cumulus cells and the oocyte forms a structural pathway for cell-to-cell communication, which allows entry of essential metabolites and instructional signalling to the oocytes (Gilula et al 1978;Chen et al 1990). Gonadotrophins stimulate the cumulus cells to secrete and produce hyaluronic acid (Chen et al 1990) and a MW 30,000 polypeptide (Moor and Crosby 1987), which causes the dispersion of cells, a process called cumulus expansion and mucification.…”

Section: Discussionmentioning

confidence: 99%

Technical Report: Effect of commercially available PMSG on maturation, fertilization and embryo development of buffalo oocytes in vitro

Gupta¹,

Nandi²,

Ravindranatha³

et al. 2001

Reprod. Fertil. Dev.

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In vitro fertilization (IVF) technology provides an opportunity to produce embryos for genetic manipulation, embryo transfer and basic research in developmental physiology, and can be exploited for emerging biotechnologies such as transgenesis and cloning. In the present study, the effects of different concentrations of commercially available pregnant mare serum gonadotrophin (PMSG) (Folligon; Intervet, International B.V, Boxmeer, Holland) in oocyte culture media, on maturation, fertilization and embryonic development of buffalo oocytes in vitro were investigated. Oocytes aspirated from abattoir-derived ovaries were cultured in media containing TCM-199 + PMSG at 0, 2.5, 20, 30, 40 and 50 IU mL(-1) in presence or absence of steer serum (10%) for 24 h in a CO2 incubator. The maturation rate was assessed on the basis of degree of expansion of cumulus cells. The matured oocytes were inseminated with 9-10 x 10(6) spermatozoa mL(-1) in Brackett and Oliphant medium and the cleavage rate was recorded 40-42 h after insemination. Uncleaved oocytes were stained with aceto-orcein for evaluation of fertilization rates. The cleaved embryos were further cultured in TCM-199 + 10% steer serum on buffalo oviducal cell monolayer for 7 days. Maturation, fertilization, cleavage and embryonic development were significantly higher (P < 0.05) in oocytes cultured in TCM-199 + 10% steer serum supplemented with 40 and 50 IU PMSG mL(-1). It is concluded that commercially available PMSG can effectively be used in place of pure follicle-stimulating hormone for in vitro maturation of buffalo oocytes, making it cost effective for IVF studies.

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“…A further defect shown by embryos derived from in vitro-matured oocytes is represented by the failure of zygotes to progress to the blastocyst stage (Motlik and Fulka, 1974;Nagai et al, 1984;Toyoda et al, 1984;First et al, 1988;Parrish, 1991;Rath, 1991 ). Recent Moor, 1984;Mattioli et al, 1988a;1989;Galeati et al, 1991 Moreover, the addition of LH and FSH to the culture medium facilitated and accelerated meiotic progression, and LH selectively improved cytoplasmatic maturation (Crosby et al, 1981; Gonadotrophins not only support oocyte maturation, but also induce major alterations in the protein profile of follicle and cumulus cells (Ball et al, 1985; Moor and Crosby, 1987). …”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of the polypeptide pattern of cumulus cells during maturation of porcine cumulus oocyte complexes in vivo and in vitro

Schroeter¹,

Meinecke²

1995

Reprod. Nutr. Dev.

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Cellular origin, hormonal regulation and biochemical characteristics of polypeptides secreted by Graafian follicles of sheep

Abstract: Summary. The

Cited by 15 publications

References 18 publications

Qualitative and quantitative changes in protein sythesis of bovine follicular cells during the preovulatory period

Qualitative and quantitative changes in protein sythesis of bovine follicular cells during the preovulatory period

Technical Report: Effect of commercially available PMSG on maturation, fertilization and embryo development of buffalo oocytes in vitro

Comparative analysis of the polypeptide pattern of cumulus cells during maturation of porcine cumulus oocyte complexes in vivo and in vitro

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