Cellular Performance Comparison of Biomimetic Calcium Phosphate Coating and Alkaline-Treated Titanium Surface

Wei, Mei

doi:10.1155/2013/832790

Cited by 10 publications

(7 citation statements)

References 51 publications

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“…The accelerated mineral deposition observed on PLAGA/n-HA scaffolds implied that incorporation of hydroxyapatite into the microsphere sintered scaffolds could improve their bioactivity in term of mineral deposition. The newly formed apatite layer showed characteristic morphology of tightly packed aggregation of nano particles, which has been observed by other researchers [24][25][26]. This unique morphology combined with Alizarin red staining confirmed that the observed mineral layer was formed during SBF incubation instead of the n-HA incorporated during scaffold fabrication.…”

Section: Rmscs Culturesupporting

confidence: 81%

Evaluation of PLAGA/n-HA Composite Scaffold Bioactivity in vitro

Lv¹,

Deng²,

Nair³

et al. 2014

Bioceram Dev Appl

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Polymeric sintered microsphere scaffolds have shown their tremendous potential in bone tissue engineering applications due to their highly porous and interconnected three dimensional structure and excellent mechanical properties. While these scaffolds are able to support basic cellular activity after seeding cells on them, the bioactivity of these scaffolds in terms of enhancing the biological performance of stem cells during bone regeneration is still under satisfactory. We hypothesized that incorporation of bioactive addictive such as hydroxyapatite into these scaffolds could improve their bioactivity without sacrificing the bulk properties of the scaffolds. We have successfully incorporated nano-hydroxyapatite (n-HA) into poly (lactic acid-glycolic acid) (PLAGA) microsphere based scaffolds in our previous studies. Herein, we aimed to evaluate the bioactivity of PLAGA/n-HA composite scaffolds, with a focus on studying the mineralization of the scaffolds in vitro. The capability of inducing apatite formation in vitro was largely enhanced in the composite scaffolds compared to plain PLAGA scaffolds. More importantly, PLAGA/n-HA composite scaffolds have been shown to improve rabbit mesenchymal stem cells (RMSCs) proliferation, differentiation, and mineralization as compared to control PLAGA scaffolds. Taken together, introduction of n-HA appears to be an efficient approach to improve the bioactivity of PLAGA scaffolds for bone tissue engineering.

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Section: Rmscs Culturesupporting

confidence: 81%

Evaluation of PLAGA/n-HA Composite Scaffold Bioactivity in vitro

Lv¹,

Deng²,

Nair³

et al. 2014

Bioceram Dev Appl

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“…After fixation with 3.7% paraformaldehyde for 15 min and permeabilization with 0.2% Triton X-100 in phosphate buffer solution for 10 min, the slides were incubated with blocking buffer (3% BSA, 0.1% Tween 20 in Tris buffer solution). Then the slides were incubated with Alexa Fluor 568 phalloidin to detect F-actin, and PI was employed to detect nuclei [ 11 ]. Labeled cells were examined under a Zeiss confocal microscope, and images were obtained with the laser scanning confocal microscope LEICA TCS SP2 (Leica, Wetzlar, Germany).…”

Section: Methodsmentioning

confidence: 99%

Rho/ROCK Signal Cascade Mediates Asymmetric Dimethylarginine-Induced Vascular Smooth Muscle Cells Migration and Phenotype Change

Zhou

Lan

Guo

et al. 2014

BioMed Research International

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Asymmetric dimethylarginine (ADMA) induces vascular smooth muscle cells (VSMCs) migration. VSMC phenotype change is a prerequisite of migration. RhoA and Rho-kinase (ROCK) mediate migration of VSMCs. We hypothesize that ADMA induces VSMC migration via the activation of Rho/ROCK signal pathway and due to VSMCs phenotype change. ADMA activates Rho/ROCK signal pathway that interpreted by the elevation of RhoA activity and phosphorylation level of a ROCK substrate. Pretreatment with ROCK inhibitor, Y27632 completely reverses the induction of ADMA on ROCK and in turn inhibits ADMA-induced VSMCs migration. When the Rho/ROCK signal pathway has been blocked by pretreatment with Y27632, the induction of ERK signal pathway by ADMA is completely abrogated. Elimination of ADMA via overexpression of dimethylarginine dimethylaminohydrolase 2 (DDAH2) and L-arginine both blocks the effects of ADMA on the activation of Rho/ROCK and extra cellular signal-regulated kinase (ERK) in VSMCs. The expression of differentiated phenotype relative proteins was reduced and the actin cytoskeleton was disassembled by ADMA, which were blocked by Y27632, further interpreting that ADMA inducing VSMCs migration via Rho/ROCK signal pathway is due to its effect on the VSMCs phenotype change. Our present study may help to provide novel insights into the therapy and prevention of atherosclerosis.

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“…Various approaches have been explored to improve the properties of bioceramic, such as incorporation of sintering additives [ 4 ], polarization [ 5 ] and surface coating [ 6 , 7 ]. Introducing trace minerals into bioceramic has drawn scientific interest since it is effective in improving the mechanical properties and biological responses [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Nano SiO2 and MgO Improve the Properties of Porous β-TCP Scaffolds via Advanced Manufacturing Technology

Gao

Wei

Feng

et al. 2015

IJMS

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Nano SiO2 and MgO particles were incorporated into β-tricalcium phosphate (β-TCP) scaffolds to improve the mechanical and biological properties. The porous cylindrical β-TCP scaffolds doped with 0.5 wt % SiO2, 1.0 wt % MgO, 0.5 wt % SiO2 + 1.0 wt % MgO were fabricated via selective laser sintering respectively and undoped β-TCP scaffold was also prepared as control. The phase composition and mechanical strength of the scaffolds were evaluated. X-ray diffraction analysis indicated that the phase transformation from β-TCP to α-TCP was inhibited after the addition of MgO. The compressive strength of scaffold was improved from 3.12 ± 0.36 MPa (β-TCP) to 5.74 ± 0.62 MPa (β-TCP/SiO2), 9.02 ± 0.55 MPa (β-TCP/MgO) and 10.43 ± 0.28 MPa (β-TCP/SiO2/MgO), respectively. The weight loss and apatite-forming ability of the scaffolds were evaluated by soaking them in simulated body fluid. The results demonstrated that both SiO2 and MgO dopings slowed down the degradation rate and improved the bioactivity of β-TCP scaffolds. In vitro cell culture studies indicated that SiO2 and MgO dopings facilitated cell attachment and proliferation. Combined addition of SiO2 and MgO were found optimal in enhancing both the mechanical and biological properties of β-TCP scaffold.

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Cellular Performance Comparison of Biomimetic Calcium Phosphate Coating and Alkaline-Treated Titanium Surface

Cited by 10 publications

References 51 publications

Evaluation of PLAGA/n-HA Composite Scaffold Bioactivity in vitro

Evaluation of PLAGA/n-HA Composite Scaffold Bioactivity in vitro

Rho/ROCK Signal Cascade Mediates Asymmetric Dimethylarginine-Induced Vascular Smooth Muscle Cells Migration and Phenotype Change

Nano SiO2 and MgO Improve the Properties of Porous β-TCP Scaffolds via Advanced Manufacturing Technology

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