Cellular thermotolerance is associated with heat shock protein 70.1 genetic polymorphisms in Holstein lactating cows

Basiricò, Loredana; Morera, P.; Primi, Valentina; Lacetera, Nicola; Nardone, A.; Bernabucci, U.

doi:10.1007/s12192-011-0257-7

Cited by 127 publications

(94 citation statements)

References 35 publications

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“…We thought that these contradictions might arise from complications of thermotolerance and neuronal death mechanisms. 26,27) Thus, additional research such as pharmacological inhibition of caspase-3 or genetic knock-down will be necessary to establish the correlation between HSP70, caspase-3, and dopaminergic neuron dysfunction. However, we ascertained time-dependent effects of heat stress on HSP70 expression and caspase-3 activation in the SN of the mouse brain.…”

Section: Discussionmentioning

confidence: 99%

Repeated Heat Exposure Impairs Nigrostriatal Dopaminergic Neurons in Mice

Kim

Park

et al. 2013

Biological & Pharmaceutical Bulletin

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Environmental heat stress is associated with physical stress responses, including changes in monoamines, protein expression, and neuronal circuits and damage to neurons in the brain. This study determined the effects of heat stress on the nigrostriatal dopaminergic system based on behavioral, histological, and neurochemical analyses. To evaluate behavioral changes after heat exposure, we subjected mice to the pole and open field tests. The data suggested that heat stress for 7 d significantly impaired movement. Then, we conducted a histological analysis using tyrosine hydroxylase (TH) immunoreactivity in the striatum and substantia nigra (SN). Heat stress induced a significant deficit in TH-positive fibers and cells after 14-and 21-d exposure, respectively. We also measured the striatal dopamine (DA), 4-hydroxy-3-methoxy-phenylacetic acid, and 3,4-dihydroxyphenylacetic acid levels. The data suggested that DA turnover rate increased with heat exposure in a time-dependent manner, resulting in the significant decrease of DA after 28 d. Moreover, the expression of heat shock protein 70 (HSP70) was increased in the mouse SN with up to 14-d heat exposure, but decreased after 21 d of the stress. And glucose-regulated protein 78 (GRP78) was gradually increased in the mouse SN with 28-d heat exposure. The caspase-3 activity was also increased after 14-d heat exposure. These findings are the first evidence that repeated heat stress impairs nigrostriatal dopaminergic neurons, motor function, and DA availability with changes of HSP70 and GRP78 expressions and caspase-3 activity in mice.Key words repeated heat stress; dopaminergic neuron; heat shock protein 70; glucose-regulated protein 78; caspase-3 Extreme environmental temperatures induce systemic metabolic disorders accompanied by hyperthermia, behavioral, physiological, and biochemical changes, which might be considered pathophysiological indices for assessing heat stress.

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Section: Discussionmentioning

confidence: 99%

Repeated Heat Exposure Impairs Nigrostriatal Dopaminergic Neurons in Mice

Kim

Park

et al. 2013

Biological & Pharmaceutical Bulletin

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“…33 The presence of SNPs in the 5′-UTR region of inducible HSP70 showed amelioration of heat stress response and tolerance to heat stress in bovines. These mutation sites would be serving as the appropriate molecular genetic markers for thermal tolerance.…”

Section: 52mentioning

confidence: 98%

“…56 The association between inducible HSP70.1 single nucleotide polymorphisms (SNPs) and the HS response of peripheral blood mononuclear cells, (PBMC) was studied in dairy cows by genotyping 446 Italian Holstein cows. 33 The presence of SNPs in the 5′-UTR region of inducible HSP70 showed amelioration of heat stress response and tolerance to heat stress in bovines. These mutation sites would be serving as the appropriate molecular genetic markers for thermal tolerance.…”

Section: Types Of Hsps Studied In Livestock During Hsmentioning

confidence: 98%

Section: Mechanism Of Hsp Expressionmentioning

confidence: 99%

“…33 Gene networks within and across cells and tissues respond to HS through intracellular and extracellular signals with gene expression changes in which activation of heat shock transcription factor (HSF1) and increased expression of HSP have been widely studied in most of the organisms including bovine, cattle and mice. 13 Upon heat stimulus, the HSF1 monomer previously bound to HSP during unstressed condition in the cytoplasm, get dissociated and bind with other HSF monomers for trimerization before their nuclear translocation.…”

Section: Mechanism Of Hsp Expressionmentioning

confidence: 99%

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Role of Heat Shock Proteins in Livestock Adaptation to Heat Stress

PR¹

2017

JDVAR

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The present review is an attempt to signify the importance of heat shock proteins in livestock adaptation during heat stress. The cellular and molecular responses in livestock are very crucial as it may lead to identification of confirmatory biomarker for heat stress in livestock. Thermo-tolerant gene expression and elevated heat shock protein (HSP) levels are observed to be the ultimate response through which the cell survives the heat stress. The HSPs have chaperonic activity ensuring the folding, unfolding and refolding of stress-denatured proteins. The components of heat shock response include heat shock factors (HSFs), heat shock element (HSE) and HSP. The cellular response to heat stress in mammalian organisms is controlled at the transcription level and it is mediated by a family of HSF which are regulated by the corresponding HSF genes. The activated HSFs bind with the HSE in the promoter region of HSP genes culminating in enhanced transcription of HSP mRNA. The HSP70, HSP90 and HSP27 are the predominant HSPs having protective role during heat stress in farm animals. Among these HSPs studied, HSP70 was identified to be the ideal biological marker for quantifying heat stress in animals.Keywords: heat stress, heat shock factors, heat shock response, hsp70, livestock Journal of Dairy, Veterinary & Animal Research Review Article Open AccessRole of heat shock proteins in livestock adaptation to heat stress the latter as it is harder to find an environment with a single variable changing. However, both these mechanisms are phenotypic responses as it is induced by the environment and the response goes once the stress is removed. It is usually produced to improve the fitness of the animal to the environment. Animals develop specific adaptive mechanisms if the environment stressors are present for a prolonged period. The mechanisms of adaptation include morphological, behavioural, biochemical and physiological changes. Biochemical adaptation to thermal stress involves changes in proteins, membrane lipids and metabolic rate.7 Genetic markers are of prior importance in the recent revolutionary developments in the field of molecular technology because of its viability as the biochemical adaptation can be utilized in livestock breeding programs compared to the behavioral, morphological or physiological responses to stress which is of less relevance. Heat shock protein is an important biomarker produced as cellular and tissue defense mechanism whose expression is markedly increased during heat shock. 8 The present review is an attempt to signify the importance of heat shock proteins in livestock adaptation during HS. General cellular responses to heat stressCellular exposure to thermal stress induces a number of anomalies in the functioning of cells which alters the biological molecules, disturbs cell functions, modulates metabolic reactions, induces oxidative cell damage and activates both apoptosis and necrosis pathways, ultimately leading to cell survival, acclimation or cell death depending on the time and...

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