Cellular uptake, intracellular behavior, and acute/sub-acute cytotoxicity of a PEG-modified quantum dot with promising in-vivo biomedical applications

Cheng, Qingyuan; Duan, Yiping; Fan, Wei; Li, Dongxu; Zhu, Cuiwen; Ma, Tiantian; Liu, Jie; Yu, Mingxia

doi:10.1016/j.heliyon.2023.e20028

Cited by 1 publication

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References 55 publications

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“…Previously Bentzen et al [17] showed that by coating amphiphilic poly(acrylic acid) ((AMP)-capped) CdSe/ZnS with polyethylene glycol (PEG) (MW 2000) the nonspecific binding to cells was reduced. In the literature, there were several other studies using PEG to functionalize QDs [18][19][20][21]. Here we investigate the use of PEG to enhance aqueous CdPbS QD suspension stability and reduce nonspecific binding to cells.…”

Section: Introductionmentioning

confidence: 99%

PEGylation of NIR Cd_0.3Pb_0.7S aqueous quantum dots for stabilization and reduction of nonspecific binding to cells

Jednorski,

Acar,

Shih

et al. 2024

Biomed. Mater.

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Cd0.3Pb0.7S (CdPbS) aqueous quantum dots (AQDs) made with 3-mercaptoproprionic acid (MPA) as a ligand have the advantages of emitting near infrared (NIR) light, well above 800 nm, that completely circumvents interference from tissue autofluorescence and have significant amounts of ligands for bioconjugation. However, retaining the right amount of MPA became a challenge when using CdPbS AQDs for bioimaging because retaining too much MPA could lead to significant nonspecific staining in cell imaging while insufficient MPA could cause AQDs instability in biological systems. Here we examined PEGylation (i.e., chemically linking aminefunctionalized polyethylene glycol (PEG)) to modify MPA on the AQDs surface to improve AQDs stability and reduce nonspecific staining. In addition, for conjugation with antibodies, a bifunctional PEG with a carboxyl functionality was used to permit chemical linkage of a PEG to an antibody on the other end. It was found that performing PEGylation at the thiol concentration where the zeta potential becomes saturated stabilized the CdPbS AQDs suspension and reduced nonspecific binding to cells. Furthermore, with the bifunctional PEG, the CdPbS AQDs were conjugated with antibodies and the AQD-Ab conjugates were shown to stain cancer cells specifically against normal cells with a signal-to-noise ratio of 8.

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Section: Introductionmentioning

confidence: 99%

PEGylation of NIR Cd_0.3Pb_0.7S aqueous quantum dots for stabilization and reduction of nonspecific binding to cells

Jednorski,

Acar,

Shih

et al. 2024

Biomed. Mater.

View full text Add to dashboard Cite

show abstract

Cellular uptake, intracellular behavior, and acute/sub-acute cytotoxicity of a PEG-modified quantum dot with promising in-vivo biomedical applications

Cited by 1 publication

References 55 publications

PEGylation of NIR Cd_0.3Pb_0.7S aqueous quantum dots for stabilization and reduction of nonspecific binding to cells

PEGylation of NIR Cd_0.3Pb_0.7S aqueous quantum dots for stabilization and reduction of nonspecific binding to cells

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Cellular uptake, intracellular behavior, and acute/sub-acute cytotoxicity of a PEG-modified quantum dot with promising in-vivo biomedical applications

Cited by 1 publication

References 55 publications

PEGylation of NIR Cd0.3Pb0.7S aqueous quantum dots for stabilization and reduction of nonspecific binding to cells

PEGylation of NIR Cd0.3Pb0.7S aqueous quantum dots for stabilization and reduction of nonspecific binding to cells

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PEGylation of NIR Cd_0.3Pb_0.7S aqueous quantum dots for stabilization and reduction of nonspecific binding to cells

PEGylation of NIR Cd_0.3Pb_0.7S aqueous quantum dots for stabilization and reduction of nonspecific binding to cells