Cellulase and Pectinase Production Potentials of <em>Aspergillus Niger</em> Isolated from Corn Cob

Abstract

Production of pectinase and cellulase by Aspergillus niger from corn cob was examined. The organism was screened for enzymatic activity using Carboxyl Methyl Cellulose (CMC) and Pectin as substrate. The result revealed a clear zone of inhibition in the agar plates. The organism was subjected to different optimum conditions which include pH, temperature, biomass yield and enzymatic activity. The highest cellulose activity was obtained on the 4 th day (1.9×10 -4 µg/ml/sec) while the highest pectinase activity (1.5×10 -4 µg/ml/sec) was obtained on the 4 th and 5 th day. The optimum pH for cellulase production was pH 4 with an activity of 2.70×10 -4 µg/ml/sec while the optimum pH for pectinase activity was pH 6 with an activity of 1.5×10 -4 µg/ml/sec. The optimum temperature was at 50 o C with an enzyme activity of 1.3x10 -4 µg/ml/sec for cellulase production while the optimum temperature of 60 0 C gave the highest pectinase activity of 1.6x10 -4 µg/ml/sec. This study revealed that Aspergillus niger from corn cob has the ability to produce cellulase and pectinase, hence it may be considered as a source for the production of industrial cellulase and pectinase.

Cloning and characterization of an endo--1,4-xylanase gene from Colletotrichum lindemuthianum and phylogenetic analysis of similar genes from phytopathogenic fungus

“…Accordingly, though maximum growth was obtained on glucose, a readily metabolizable nutrient, basal expression of xyl1 and only trace amounts of XYL were produced by both races; however, although low, a higher XYL production was observed for race 1472, particularly early during incubation. A similar basal production of xylanase and endoglucanase (Sachslehner et al, 1998;Tuncer et al, 2004), pectinases (Hernández-Silva et al, 2007;Oyeleke et al, 2012;Riou et al, 1991) and cellulases (AcostaRodriguez et al, 2005;Carle-Urioste et al, 1997;Sharada et al, 2013) has been described in the fungi S. rolsfii, S. sclerotiorum and C. lindemuthianum, respectively.…”

Properties of &lt;i&gt;Bacillus&lt;/i&gt; species Cellulase Produced Using Cellulose from Brewers Spent Grain (BSG) as Substrate

“…There are studies on cellulase from microorganisms associated with the gut of giant African snail (Archachatina marginata) [4]. It was documented that a cellulase activity of 0.62 Mg/ml/sec and optimum pH 80˚C for Bacillus subtilis cellulase.…”

Evaluation of Enzyme Cellulase Production and it's Activity, Isolated from Local Fungi

“…Species were identified by examining the development of colonies by the light microscope. Depending on the shape and color of the colonies in addition to the microscopic examination of the branches of mycelium and spores formed for identification of fungi [9,10] . A pure sample of the studied species was kept a slope in the test tubes in the fridge at 4 o C, the samples were renewed every two weeks to maintain the vitality and effectiveness of the fungal growth.…”

“…The economic importance of the cellulase enzyme produced by microorganisms such as Trichoderma ,Aspergillus , Cladosporium, brought attention for development and designing of different types of researches [4,9] .…”