Cellulase and protein production by Chaetomium cellulolyticum strains grown on cellulosic substrates

Fähnrich, P.; Irrgang, Klaus−Dieter

doi:10.1007/bf00154645

Cited by 19 publications

(1 citation statement)

References 8 publications

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“…Protein concentration decreased with increasing the substrate concentration. This is possibly due to a partial adsorption of enzymes to the substrate (Fahnrich and Irrgang, 1981; Szczodrak, 1988). Surprisingly, the activity of β ‐glucosidase increased with increasing the substrate concentration.…”

Section: Resultsmentioning

confidence: 99%

Enzyme production by the mixed fungal culture with nano‐shear pretreated biomass and lignocellulose hydrolysis

Weerasiri

Liu

et al. 2013

Biotech & Bioengineering

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Cellulase, xylanase, and β-glucosidase production was studied on novel nano-shear pretreated corn stover by the mixed fungi culture. The high shear force from a modified Tayor-Couette nano-shear mixing reactor efficiently disintegrated corn stover, resulting in a homogeneous watery mash with particles in much reduced size. Scanning electron microscope study showed visible mini-pores on the fiber cell wall surface, which could improve the accessibility of the pretreated corn stover to microorganisms. Mixed fungal culture of Trichoderma reesei RUT-C30 and Aspergillus niger produced enzymes with higher cellulolytic and xylanolytic activities on corn stover pretreated with nano-shear mixing reactor, in comparison with other pretreatment methods, including acid and ammonia fiber explosion (AFEX) pretreatment. The hydrolytic potential of the whole fermentation broth from the mixed fungi was studied, and the possibility of applying the whole cell saccharification concept was also investigated to further reduce the cost of lignocellulose hydrolysis.

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Section: Resultsmentioning

confidence: 99%

Enzyme production by the mixed fungal culture with nano‐shear pretreated biomass and lignocellulose hydrolysis

Weerasiri

Liu

et al. 2013

Biotech & Bioengineering

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Production of cellulases and xylanase by Trichoderma reesei F‐522 on pretreated wheat straw

Szczodrak

1988

Acta Biotechnologica

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Autohydrolyzed and ethanol-alkali pulped wheat straw w e examined as a candidate feedstock for both cellulase and xylanase production and enzymatic hydrolysis. Submerged cultures of Trichoderma reesei F-522 grown on hydrothermally modified straw provided culture supernatants of the highest enzymatic activities, whereas the maximal efficiency of enzymatic hydrolysis was recorded in straw treated with ethanol-NaOH mixture. Some culture conditions were optimized to improve the growth and cellulase production by T. reesei on autohydrolyzed wheat straw.

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Induction of Cellulases in chaetomium cellulolyticum by cellobiose

Leisola

Ulmer

Pitkänen

et al. 1985

Biotech & Bioengineering

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The production of extracellular cellulases by Chaetomium cellulolyticum could be induced by slow feeding of cellobiose to the cultures. Both the rate of production and the amount of activity were comparable to that obtained in batch cultivation on cellulose. The specific filter paper activity of 2.06 U per mg protein was almost two times higher than that obtained in cellulose medium. Cellulases were not induced when glucose was slowly fed to the cultures. Changing the feed stream from glucose to cellobiose resulted in a rapid accumulation of cellulases. Thus cellobiose has a similar role in cellulase induction in C. cellulolyticum, as earlier shown for Trichoderma reesei.

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Cellulase and protein production by Chaetomium cellulolyticum strains grown on cellulosic substrates

Cited by 19 publications

References 8 publications

Enzyme production by the mixed fungal culture with nano‐shear pretreated biomass and lignocellulose hydrolysis

Enzyme production by the mixed fungal culture with nano‐shear pretreated biomass and lignocellulose hydrolysis

Production of cellulases and xylanase by Trichoderma reesei F‐522 on pretreated wheat straw

Induction of Cellulases in chaetomium cellulolyticum by cellobiose

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