Cellulase Production by Aspergillus flavus Linn Isolate NSPR 101 fermented in sawdust, bagasse and corncob

Ojumu, Tunde Victor; Solomon, B. O.; Betiku, Eriola; Layokun, S. K.; Amigun, Bamikole

doi:10.5897/ajb2003.000-1030

Cited by 96 publications

(58 citation statements)

References 11 publications

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“…Our results are comparable to Ojumu et al [23] who reported high cellulase activity from 3% pretreated saw dust, bagasse and corn cob as substrate respectively. Substrate concentration is a dynamic influencing feature that affects the yield and initial hydrolysis rate of cellulose [24].…”

Section: Production Of Cellulasesupporting

confidence: 80%

Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility

Iqbal¹,

Ahmed²,

Zia³

et al. 2011

ABB

111

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This paper reports the purification and characterization of kinetic parameters of cellulase produced from Trichoderma viride under still culture solid state fermentation technique using cheap and an easily available agricultural waste material, wheat straw as growth supported substrate. Trichoderma viride was cultured in fermentation medium of wheat straw under some previously optimized growth conditions and maximum activity of 398 ± 2.43 U/mL obtained after stipulated fermentation time period. Cellulase was purified 2.33 fold with specific activity of 105 U/mg in comparison to crude enzyme extract using ammonium sulfate precipitation, dialysis and Sephadex-G-100 column chromatography. The enzyme was shown to have a relative low molecular weight of 58 kDa by sodium dodecyl sulphate poly-acrylamide gel electrophoresis. The purified enzyme displayed 6.5 and 55˚C as an optimum pH and temperature respectively. Using carboxymethyl cellulose as substrate, the enzyme showed maximum activity (V max ) of 148 U/mL with its corresponding K M value of 68 µM. Among activators/inhibitors SDS, EDTA, and Hg 2+ showed inhibitory effect on purified cellulase whereas, the enzyme activated by Co 2+ and Mn 2+ at a concentration of 1 mM. The purified cellulase was compatible with four local detergent brands with up to 20 days of shelf life at room temperature suggesting its potential as a detergent additive for improved washing therefore, it is concluded that it may be potentially useful for industrial purposes especially for detergent and laundry industry.

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Section: Production Of Cellulasesupporting

confidence: 80%

Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility

Iqbal¹,

Ahmed²,

Zia³

et al. 2011

ABB

111

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“…Cellulose is the major component of plant biomass and the major biopolymer found in abundance on earth, and much of the cellulose exists as wastes. Such wastes include straw, corn cobs, wood wastes, peat, bagasse and waste paper [3]. In principle, all lignocellulosics can be converted into simple sugars which can serve as useful raw materials in the production of fuel, animal feedstock and feedstock for chemical synthesis [4].…”

Section: öZetmentioning

confidence: 99%

Purification and characterisation of a cellulase obtained from cocoa (Theobroma cacao) pod-degrading Bacillus coagulans Co4

Adeleke¹,

Omafuvbe²,

Adewale³

et al. 2012

Turk J Bioch

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Objective: Some properties of cellulase purified from the culture supernatant of Bacillus coagulans Co4, isolated from cocoa pod dumpsite were investigated for possible biotechnological applications. Methods: The crude cellulase was purified to apparent homogeneity using a combination of acetone precipitation, CM Sepharose CL-6B ion exchange chromatography and gel filtration on Sephadex G-100. The molecular and thermodynamic properties of the purified enzyme were studied following standard procedures. Results: The specific activity of the purified cellulase rose from 0.10 to 47 units/mg of protein, at the end of purification. The molecular weight was found to be 14.5 kDa; and an apparent K m value of 0.18±0.06 mg/ml of carboxylmethylcellulose. The optimum pH and temperature were 7.5 and 60 o C respectively. The activation energy for carboxylmethylcellulose hydrolysis (E a ) was 16.5 kJ/mol. Na + and K + had no effects on its activity at concentrations up to 200 mM, whereas Ca 2+ and Mg 2+ served as inhibitors at concentrations above 25 and 40 mM respectively. The cellulase retained 40% residual activity when heated at 60 o C for 40 minutes. Conclusison: On the basis of these properties, it is concluded that the purified cellulase is moderately thermostable and may have applications in the bioconversion of agricultural wastes into economically useful products.

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“…A. flavus BS1 competently utilized different lignocellulosic substrates and supported higher levels of cellulase activity [61]. A. flavus Linn NSPR 101 showed the production of cellulase on various natural substrates like bagasse, corncob and sawdust [62]. Thus, production of cellulase may vary between species of fungi; and from the above studies, it is evident that the wide range and higher enzyme activities are possible on waste lignocellulosic biomass as substrate.…”

Section: Solid-state Fermentationmentioning

confidence: 97%

An Overview on Fungal Cellulases with an Industrial Perspective

Sreedharan¹

2016

J Nutr Food Sci

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Lignocellulose is the most abundant biopolymer available on earth. Hydrolysis of lignocellulose into fermentable sugars, sugar acids and phenolics is the pre-requisite for its successful exploitation as substrates for the large scale production of industrially significant value added products. Though remarkable collection of lignocellulolytic microorganisms has been brought to limelight, only a few especially fungi have been studied extensively as they secrete copious lignocellulolytic enzymes extracellularly. Enzymatic hydrolysis of lignocelluloses is carried out by a group of enzymes viz., cellulases, hemicellulases, ligninases in unison or individually, and are collectively known as lignocellulolytic enzymes. In this light of this background, followed by a short introduction on lignocellulose, lignocellulolytic enzymes and mainly focused on fungal cellulase, this review discusses recent approaches on the production of cellulase by submerged and solid-state fermentation strategies; current knowledge on the purification, characterisation of cellulase; and finally concluded with detailed industrial applications of fungal cellulase.

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Cellulase Production by Aspergillus flavus Linn Isolate NSPR 101 fermented in sawdust, bagasse and corncob

Cited by 96 publications

References 11 publications

Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility

Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility

Purification and characterisation of a cellulase obtained from cocoa (Theobroma cacao) pod-degrading Bacillus coagulans Co4

An Overview on Fungal Cellulases with an Industrial Perspective

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