Cellulase production in a new mutant strain of Penicillium decumbens ML-017 by solid state fermentation with rice bran

Abstract

To produce cellulolytic enzyme efficiently, Penicillium decumbens strain L-06 was used to prepare mutants with ethyl methane sulfonate (EMS) and UV-irradiation. A mutant strain ML-017 is shown to have a higher cellulase activity than others. Box-Behnken's design (BBD) and response surface methodology (RSM) were adopted to optimize the conditions of cellulase (filter paper activity, FPA) production in strain ML-017 by solid-state fermentation (SSF) with rice bran as the substrate. And the result shows that the initial pH, moisture content and culture temperature all have significant effect on the production of cellulase. The optimized condition shall be initial pH 5.7, moisture content 72% and culture temperature 30°C. The maximum cellulase (FPA) production was obtained under the optimized condition, which is 5.76 IU g(-1), increased by 44.12% to its original strain. It corresponded well with the calculated results (5.15 IU g(-1)) by model prediction. The result shows that both BBD and RSM are the cellulase optimization methods with good prospects.

ESTUDO DA INFLUÊNCIA DO pH E DA TEMPERATURA NA PRODUÇÃO DE CELULASES POR Mucor circinelloides

“…RCK 2010, encontrando maior atividade enzimática em pH 5,5 e 30 °C, sendo este resultado dez vezes inferior ao encontrado no presente estudo. Resultado semelhante (pH 5,7 e 30 °C) foi observado por Liu et al (2011), na produção de celulases por Penicillium decumbens ML-017.…”

Valorization of Cereal Based Biorefinery Byproducts: Reality and Expectations

“…62 A high yield (159.1 U/g) of β-glucosidase was achieved from rice bran-based SSF by the Penicillium citrinum fungus. 40 Also, Liu et al 63 optimized the conditions of cellulase production by Penicillium decumbens in SSF using rice bran as the substrate and obtained 5.76 IU/g, whereas Lee et al 64 used rice bran in combination with yeast extract to produce the same enzyme in SmF by Bacillus subtilis and 196.8 U/mL was obtained. Different agro-industrial residues were used for the production of this vital enzyme such as enzymatically treated wheat bran which exhibited 1.1 U/mL of the cellulase enzyme under SmF by Aspergillus flavus , 65 or untreated corn cob which produced 5.25 IU/mL under SmF by Trichoderma reesei.…”

Production of cellulases by Penicillium sp. in a solid-state fermentation of oil palm empty fruit bunch

“…Many studies reported that several Penicillium species secrete a complete cellulase with high β-glucosidase activity (Singh et al, 2009;Dillon et al, 2011;Liu et al, 2011;Singhvi et al, 2011;Castro et al, 2010;Dutta et al, 2008). Cellulase from Penicillium funiculosum exhibited better-balanced β-glucosidase, endo-and exoglucanase activities than commercial preparations had done (Castro et al, 2010), and produced glucose faster during corn cob hydrolysis than commercialized enzymes.…”

“…In addition, Penicillium citrinum produced alkali-tolerant and thermostable cellulases (Dutta et al, 2008). In order to improve the production rate and specific activity of cellulases, chemical and physical mutagenesis have been applied to several fungal strains (Dillon et al, 2011;Liu et al, 2011).…”

“…Specifically, Dillon et al (2011) employed hydrogen peroxide to generate mutants, and isolated a new Penicillium echinulatum strain showing faster cellulase secretion than the wild type. Similarly, Liu et al (2011) used ethylmethanesulfonate and UV irra-diation to mutate Penicillium decumbens and created a mutant exhibiting higher cellulase activity.…”