Cellulases and Hemicellulases from Endophytic Acremonium Species and Its Application on Sugarcane Bagasse Hydrolysis

Abstract

The aim of this work was to have cellulase activity and hemicellulase activity screenings of endophyte Acremonium species (Acremonium zeae EA0802 and Acremonium sp. EA0810). Both fungi were cultivated in submerged culture (SC) containing L -arabinose, D -xylose, oat spelt xylan, sugarcane bagasse, or corn straw as carbon source. In solid-state fermentation, it was tested as carbon source sugarcane bagasse or corn straw. The highest FPase, endoglucanase, and xylanase activities were produced by Acremonium sp. EA0810 cultivated in SC containing sugarcane bagasse as a carbon source. The highest β-glucosidase activity was produced by Acremonium sp. EA0810 cultivated in SC using D -xylose as carbon source. A. zeae EA0802 has highest α-arabinofuranosidase and α-galactosidase activities in SC using xylan as a carbon source. FPase, endoglucanase, β-glucosidase, and xylanase from Acremonium sp. EA0810 has optimum pH and temperatures of 6.0, 55 °C; 5.0, 70 °C; 4.5, 60 °C; and 6.5, 50 °C, respectively. α-Arabinofuranosidase and α-galactosidase from A. zeae EA0802 has optimum pH and temperatures of 5.0, 60 °C and 4.5, 45 °C, respectively. It was analyzed the application of Acremonium sp. EA0810 to hydrolyze sugarcane bagasse, and it was achieved 63% of conversion into reducing sugar and 42% of conversion into glucose.

Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse

“…An initial screening under different SSF conditions indicated that the highest enzyme activities of endoxylanase, β-xylosidase, endoglucanase, cellobiohydrolase I and β-glucosidase for both S4F8 and Rut C30 were recorded after 3 days of incubation on SCB (data not shown) as opposed to the 7 days typically reported for fungal SCB SSF [10,42]. …”

Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilum

“…In recent decades, the interest in cellulases and hemicellulases has increased due to the ethanol production from lignocellulosic residues (de Almeida et al 2011). Xylanases have been extensively studied for their usage in the production of hydrolysate from agro-industrial wastes, in nutritional improvements of lignocellulosic feeds, processing of food, in increasing animal feed digestibility, biobleaching of paper pulp, clarification of fruit juices and wine, the extraction of plant oil, coffee and starch (Ahmed et al 2009a).…”

Multiple analyses of microbial communities applied to the gut of the wood-feeding termite Reticulitermes flavipes fed on artificial diets

“…As regards molds, there are very few studies of Sarocladium strains in literature. De Almeida et al (De Almeida et al 2011) described two strains of Sarocladium able to produce Filter Paper-cellulase, endo-1,4-β-D-glucanase, endo-1,4-β-Dxylanase, β-glucosidase, α-arabinofuranosidase and α-galactosidase. In another study, a strain of Sarocladium fuci was found to produce α-amylase, lipase, protease and cellulase but no β-glucosidase (Zuccaro et al 2004).…”