Cellulases from two Penicillium sp. strains isolated from subtropical forest soil: production and characterization

Picart, Pere; Dı́az, Pilar; Pastor, F. I. Javier

doi:10.1111/j.1472-765x.2007.02148.x

Cited by 62 publications

(38 citation statements)

References 24 publications

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“…niger, E. nidulans, F. compactum, F. chlamydosporium, F. oxysporium, L. theobromae, P. purpurogenum, T. harzianum and T. reesei. These fungal isolates have been previously isolated from lignocellulosic materials of which wood chips is one (Immanuel et al, 2007;Picart et al, 2007). Trichoderma spp.…”

Section: Discussionmentioning

confidence: 99%

Isolation, identification and screening of xylanase and glucanase-producing microfungi from degrading wood in Nigeria

Adesina¹,

Onilude²

2013

Afr. J. Agric. Res.

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The objective of this present study was to isolate, identify and screen for potential fungal isolates from local degrading wood chips with respect to glucanase and xylanase production. The fungal strains were isolated from degrading wood chips over a period of six months. Five hundred and twenty six isolates were obtained from the different degrading wood chips with different frequencies of occurrence. Using cultural and morphological observations, the isolates were identified and grouped into thirteen fungal species. All the fungal strains were screened for their ability to produce both glucanase and xylanase by plate screening method using Congo red as indicator. Twenty isolates were further selected among them and screened for the production of the enzymes in liquid medium. Among the twenty isolates, Trichoderma reesei TRAR had the highest glucanase activity of 10.18 U/ml while Fusarium compactum FCGA had the highest xylanase activity of 9.33 U/ml.

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Section: Discussionmentioning

confidence: 99%

Isolation, identification and screening of xylanase and glucanase-producing microfungi from degrading wood in Nigeria

Adesina¹,

Onilude²

2013

Afr. J. Agric. Res.

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“…Efficient hydrolysis of cellulose requires the action of a complex of cellulolytic enzymes that work synergistically: exo-1, 4-β-D-glucanases, endo-1-4-β-D-glucanases and 1,4-β-Dglucanases [2]. The production of cellulases by fungi and bacteria is widely disseminated in nature.…”

Section: Introductionmentioning

confidence: 99%

Production of Cellulase and Xylanase by <i>Aspergillus terreus</i> KJ829487 Using Cassava Peels as Subtrates

Olanbiwoninu¹,

Odunfa²

2016

AiM

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Cassava (Manihot esculenta, Crantz) is one of the most important food plants in West Africa. Its peels are made up of cellulose, hemicellulose and lignin. This lignocellulolytic biomass can be converted using microbial enzymes to fermentable sugars which have wide range of biotechnological relevance in many fermentation processes. The aim of this study is to screen filamentous fungi from decaying cassava peels that are good producers of xylanases and cellulases. Decaying parts of cassava peels were obtained and brought to the laboratory for further work. Fungi were isolated, identified and screened for cellulase and xylanase production. Isolate with highest frequency of occurrence and enzyme production was identified using phenotypic and molecular method. Optimisation of growth conditions for enzymes production was monitored using the DNSA method, also saccharification of cassava peel were carried out using the enzymes obtained from the isolate. Aspergillus terreus KJ829487 was the predominant fungus. It produces cellulases and xylanases optimally at 40˚C, pH 6 and 8, utilising carboxymethylcellulose (CMC) or xylose and yeast extracts as its carbon and nitrogen sources respectively. Saccharification of the peels yielded 584 mg/L glucose, 78 mg/L xylose and 66 mg/L rhamnose. Aspergillus terreus KJ829487 obtained from cassava peels have the ability to produce high concentration cellulases and xylanases which effectively hydrolysed the lignocelluloses' biomass to fermentable sugars.

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“…In this study, zymograms to detect cellulolytic activity bands were performed as described previously (Picart et al, 2007), with minor modifications. Specifically, 0.1% CMC was added to 10% SDS-PAGE.…”

Section: Cellulolytic Zymographymentioning

confidence: 99%

Cellulolytic activity and structure of symbiotic bacteria in locust guts

Su¹,

Liu²,

Li³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Locusts are able to digest the cellulose of Gramineae plants, resulting in their being considered as major crop pests. To illustrate the mechanism involved in cellulose digestion, the cellulolytic activity and zymography in the gut contents of 16 locust species were determined using carboxymethyl cellulose (CMC) as substrate. The diversity of gut symbiotic bacteria was studied using denaturing gradient gel electrophoresis (DGGE). The results showed that high CMC activity was present in Acrididae gut fluid (mean 356.4 U/g proteins). Of the 5 locust species, Oxya chinensis had the highest diversity of intestinal symbiotic bacteria, characterized by the DGGE profile containing more than 20 bands of 16S rRNA. Klebsiella pneumoniae, in the gut of Locusta migratoria manilensis, was identified as the most abundant symbiotic bacterium by DNA sequencing, with a relative abundance of 19.74%. In comparison, Methylobacterium sp was the most dominant species in the Atractomorpha sinensis gut, with a relative abundance of 29.04%. The results indicated that the cellulolytic enzymes and gut microbial Cellulolytic activity of symbiotic bacteria in locust guts communities probably reflected their phylogenetic relationship with different locust species and associated feeding strategies.

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Cellulases from two Penicillium sp. strains isolated from subtropical forest soil: production and characterization

Cited by 62 publications

References 24 publications

Isolation, identification and screening of xylanase and glucanase-producing microfungi from degrading wood in Nigeria

Isolation, identification and screening of xylanase and glucanase-producing microfungi from degrading wood in Nigeria

Production of Cellulase and Xylanase by <i>Aspergillus terreus</i> KJ829487 Using Cassava Peels as Subtrates

Cellulolytic activity and structure of symbiotic bacteria in locust guts

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Cellulases from two Penicillium sp. strains isolated from subtropical forest soil: production and characterization

Cited by 62 publications

References 24 publications

Isolation, identification and screening of xylanase and glucanase-producing microfungi from degrading wood in Nigeria

Isolation, identification and screening of xylanase and glucanase-producing microfungi from degrading wood in Nigeria

Production of Cellulase and Xylanase by &lt;i&gt;Aspergillus terreus&lt;/i&gt; KJ829487 Using Cassava Peels as Subtrates

Cellulolytic activity and structure of symbiotic bacteria in locust guts

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Product

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Production of Cellulase and Xylanase by <i>Aspergillus terreus</i> KJ829487 Using Cassava Peels as Subtrates