Cellulolytic Treatment: A Competent Approach to Improve Extraction and Storage Stability of Carotenoids from Kinnow (Citrus reticulate) Peel

Abstract

The purified cellulolytic enzyme was applied in carotenoid extraction from the Kinnow mandarin peel. The results revealed that the highest yield (8.60 ± 0.44 mg/100 g peel) of carotenoids was observed in T4, a combination of 250 IU both of CMCase and Pectinase/100 g peel whereas, the minimum recovery (3.21 ± 0.17 mg/100 g peel) was found in case of T0 (control). To assess the storage stability, the extracted carotenoids were subjected to different conditions for light and temperature. When the pigment was stored at 30 °C in dark, it showed more stability than in light. Similarly, pigment stability was adversely affected by increasing storage temperature and the loss was more pronounced at higher temperature as compared to refrigeration. After freeze drying, the stability of the extracted pigment was assessed; high retention was observed when stored in darkness or under refrigerated conditions.