Cellulose acetate beads induce release of interleukin-1 receptor antagonist, but not tumour necrosis factor-α or interleukin-1β in human peripheral blood

Abstract

These results indicate that CA beads selectively induce IL-1ra release from leucocytes which should contribute to the anti-inflammatory effect of granulocyte and monocyte adsorptive apheresis with CA beads as apheresis carriers.

Clinical Response Is Associated with Elevated Plasma Interleukin-1 Receptor Antagonist During Selective Granulocyte and Monocyte Apheresis in Patients with Ulcerative Colitis

“…We have previously demonstrated in in vitro studies that IL-1ra, which binds to IL-1RI and inhibits IL-1 signaling in leukocytes [28,29], is selectively released by GMs that adhere to the Adacolumn carriers [27,30]. Further, under identical conditions, pro-inflammatory cytokines like TNF-α and IL-1β were not measurable [30]. Similarly, in this study, IL-1α and IL-1β in plasma were not detectable during GMA in these patients.…”

“…The rise in IL-1ra is attributable to interactions between granulocytes/monocytes and the column leukocytapheresis carriers and is adhesion dependent [27]. We have previously demonstrated in in vitro studies that IL-1ra, which binds to IL-1RI and inhibits IL-1 signaling in leukocytes [28,29], is selectively released by GMs that adhere to the Adacolumn carriers [27,30]. Further, under identical conditions, pro-inflammatory cytokines like TNF-α and IL-1β were not measurable [30].…”

Adhesion dependent release of hepatocyte growth factor and interleukin-1 receptor antagonist from human blood granulocytes and monocytes: Evidence for the involvement of plasma IgG, complement C3 and ?2 integrin

“…Autologous serum was also prepared by centrifugation at 800 ¥ g for 5 min at 4°C after coagulation of blood, which was collected without an anticoagulant. The CA beads in saline were autoclaved and washed with saline as described previously [11,18]. The beads were incubated with or without plasma (in RPMI 1640) for 60 min at 37°C with gentle rotation at 1 rpm.…”

“…Our recent investigations have demonstrated that CA beads selectively induce release of anti-inflammatory cytokine IL-1ra, but not pro-inflammatory cytokines like tumour necrosis factor-a (TNF-a) or IL-1b [11]. The source of IL-1ra was considered to be GMs both of which extensively adsorb to CA beads [11]. Similarly, hepatocyte growth factor (HGF), which is an important contributor to tissue repair [12][13][14] is produced by activated GMs [15,16].…”

Cytapheresis in Inflammatory Bowel Diseases: Current Evidence and Perspectives

“…– An increased production of anti-inflammatory IL-1 receptor antagonist (IL1-ra) [19, 20] and hepatocyte growth factor [21] were seen.…”