Cellulose hydrolysis by the cellulases produced by <i>Talaromyces emersonii</i> when grown on different inducing substrates

Moloney, A.P.; Considine, P. J.; Coughlan, Michael P.

doi:10.1002/bit.260250423

Cited by 42 publications

(16 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Talaromyces helices (Penicillium teleomorph) has been reported from documents surfaces. It has cellulolytic activity (Moloney et al, 2004;Chacon & Waliszewski, 2005) so its presence involves a high risk for document conservation. Cellulolytic fungi can destroy paper materials in short time (Adamo et al, 2003) hence it uses cellulose as carbon source.…”

Section: Effect Of Different Doses Of Gamma Radiation On the Fungal Cmentioning

confidence: 99%

Control of Fungal Paper Deterioration by Antifungal Drugs, Essential Oils, Gamma and Laser Irradiation

2014

Egypt. J. Bot.

View full text Add to dashboard Cite

HIS STUDY aimed to control two selective fungi (Aspergillus parasiticus and Penicillium commune, causing biodeterioration of books and documents in storage areas of libraries) using antifungal drugs, essential oils (added singly and in combination), gamma irradiation and laser radiation. The results obtained indicated that fluconazole and clotrimazole were effective against A. parasiticus and P. commune, while nystatin was of lesser effect .P. commune showed more resistance than A. parasiticus to the antifungals (MIC 60 determination). Cinnamon and clove oils were efficient antifungal agents, while garlic and thyme oils have no reasonable activities. The combination treatment revealed some synergestic interaction between garlic and thyme oils with antifungal drugs. Generally the highest control of both fungal species was shown by single treatment of clotrimazole. It was found that the two fungi responded differently against γ-irradiation .A. parasiticus was more tolerant than P .commune in dry biomass, while they were equal in γ-irradiation tolerance in the cfu / plate. The D 10 determination revealed more tolerance of P. commune than A. parasiticus. The two fungal species showed resistance in their growth parameters (cfu and dry biomass) with laser beam treatment for 1-5 min. Reductions of 75% to 95% of control was detected after 10 and 15 min exposure to laser beam. The lethal effect, however, was observed after 20 min for both fungi.

show abstract

Section: Effect Of Different Doses Of Gamma Radiation On the Fungal Cmentioning

confidence: 99%

Control of Fungal Paper Deterioration by Antifungal Drugs, Essential Oils, Gamma and Laser Irradiation

2014

Egypt. J. Bot.

View full text Add to dashboard Cite

show abstract

“…Mycelia harvested from cultures of T. emersonii strain grown at 45°C on Sabouraud dextrose agar were used to inoculate liquid nutrient media, as described by Moloney et al (1983). Liquid cultures were grown at 45°C with shaking at 220 rpm.…”

Section: Microbial Strains Cultivation Conditions and Vectorsmentioning

confidence: 99%

Cloning, Heterologous Expression, and Characterization of the Xylitol and l-Arabitol Dehydrogenase Genes, Texdh and Telad, from the Thermophilic Fungus Talaromyces emersonii

2010

View full text Add to dashboard Cite

The genes encoding xylitol dehydrogenase (Texdh) and L: -arabitol dehydrogenase (Telad) are involved in the fungal pentose pathway and were isolated from the thermophilic fungus Talaromyces emersonii, expressed in Escherichia coli, and the products purified to homogeneity. TeXDH showed activity toward xylitol and D: -sorbitol. TeLAD was active with L: -arabitol, xylitol, and D: -sorbitol. Phylogenetic analysis showed TeLAD has evolved from D: -sorbitol dehydrogenase as a result of environmental adaptation. Substrate specificity studies indicate that TeXDH is likely to have evolved from the more broadly acting TeLAD. Texdh and Telad expression was inducible by the same carbon sources responsible for induction of genes involved in biomass degradation, suggesting for the first time a coordinated regulatory control mechanism for expression of genes encoding extracellular hydrolases and intracellular metabolic genes in the pentose utilization pathways of T. emersonii. These data also suggest that TeXDH and TeLAD may be valuable in the production of xylitol, L: -arabitol, and ethanol from renewable resources rich in pentose sugars.

show abstract

“…Mycelia harvested from cultures of T. emersonii, grown at 45ºC on Sabouraud dextrose agar, were used to inoculate liquid nutrient media, as described previously (Moloney et al 1983). Liquid cultures were grown at 45ºC with shaking at 220 rpm.…”

Section: Microbial Strains Cultivation Conditions and Vectorsmentioning

confidence: 99%

Xylose reductase from the thermophilic fungus Talaromyces emersonii: cloning and heterologous expression of the native gene (Texr) and a double mutant (Texr K271R + N273D) with altered coenzyme specificity

2009

View full text Add to dashboard Cite

Xylose reductase is involved in the first step of the fungal pentose catabolic pathway. The gene encoding xylose reductase (Texr) was isolated from the thermophilic fungus Talaromyces emersonii, expressed in Escherichia coli and purified to homogeneity. Texr encodes a 320 amino acid protein with a molecular weight of 36 kDa, which exhibited high sequence identity with other xylose reductase sequences and was shown to be a member of the aldoketoreductase (AKR) superfamily with a preference for reduced nicotinamide adenine dinucleotide phosphate (NADPH) as coenzyme. Given the potential application of xylose reductase enzymes that preferentially utilize the reduced form of nicotinamide adenine dinucleotide (NADH) rather than NADPH in the fermentation of five carbon sugars by genetically engineered microorganisms, the coenzyme selectivity of TeXR was altered by site-directed mutagenesis. The TeXR K271R+N273D double mutant displayed an altered coenzyme preference with a 16-fold improvement in NADH utilization relative to the wild type and therefore has the potential to reduce redox imbalance of xylose fermentation in recombinant S. cerevisiae strains. Expression of Texr was shown to be inducible by the same carbon sources responsible for the induction of genes encoding enzymes relevant to lignocellulose hydrolysis, suggesting a coordinated expression of intracellular and extracellular enzymes relevant to hydrolysis and metabolism of pentose sugars in T. emersonii in adaptation to its natural habitat. This indicates a potential advantage in survival and response to a nutrient-poor environment.

show abstract

Cellulose hydrolysis by the cellulases produced by Talaromyces emersonii when grown on different inducing substrates

Cited by 42 publications

References 18 publications

Control of Fungal Paper Deterioration by Antifungal Drugs, Essential Oils, Gamma and Laser Irradiation

Control of Fungal Paper Deterioration by Antifungal Drugs, Essential Oils, Gamma and Laser Irradiation

Cloning, Heterologous Expression, and Characterization of the Xylitol and l-Arabitol Dehydrogenase Genes, Texdh and Telad, from the Thermophilic Fungus Talaromyces emersonii

Xylose reductase from the thermophilic fungus Talaromyces emersonii: cloning and heterologous expression of the native gene (Texr) and a double mutant (Texr K271R + N273D) with altered coenzyme specificity

Contact Info

Product

Resources

About