CENPO is Associated with Immune Cell Infiltration and is a Potential Diagnostic and Prognostic Marker for Hepatocellular Carcinoma

Abstract

Purpose To examine the expression, clinical significance, and potential regulatory mechanism of centromere protein O (CENPO) in hepatocellular carcinoma (HCC). Methods CENPO expression in pan-cancer was studied using the TCGA-GTEx database, in HCC and normal liver tissues using the GEO and TCGA databases, and in clinical HCC samples by RT-qPCR. The diagnostic value of CENPO was assessed using receiver operating characteristic curves. Univariate and multivariate regression analyses of factors associated with HCC prognosis were performed. CENPO function and its mechanism in HCC were explored using GO, KEGG, and GSEA analyses of differentially expressed genes (DEGs). Association of CENPO expression with immune cell infiltration and immune checkpoint-associated molecules was conducted using TCGA data and the TIMER2.0 database. Relationships between CENPO expression and DNA methylation were analyzed using the UALCAN and cBioPortal databases. CENPO expression in HCC cell lines was detected using RT-qPCR. Results CENPO is upregulated in most cancers, including HCC and cell lines, and is a potential biomarker for HCC diagnosis (AUC = 0.936, 95% CI: 0.911–0.960). Higher CENPO expression was associated with poorer outcomes in patients with HCC (OS, p = 0.004; DSS, p = 0.002; PFI, p < 0.001), and CENPO was an independent predictor of factors influencing overall survival in HCC. DEGs between samples with high and low CENPO levels were enriched in various biological processes, including activation of the G2M checkpoint and other signaling pathways, while CENPO expression correlated with HCC immune cell infiltration and immune checkpoint-associated molecules, as well as CENPO promoter methylation (p < 0.001). Conclusion In HCC and cell lines, CENPO is overexpressed, a potential diagnostic marker and an indicator of poor prognosis. CENPO may regulate HCC development by influencing nuclear division and tumor immune infiltration and is regulated by methylation, making it a potential target for HCC immunotherapy.

High Expression of DNTTIP1 Predicts Poor Prognosis in Clear Cell Renal Cell Carcinoma

“…41 G2M checkpoint had a significant effect on cell proliferation, especially tumors. [42][43][44] Stabilizing Snail1 could regulate metastasis and chemoresistance of triple-negative breast cancer by. 45 MKI67 and MMPs could promote the progress of tumor, were the potential biomarker for clinical examination of malignant characteristics in tumors.…”

Advances in lung adenocarcinoma: A novel perspective on prognoses and immune responses of CENPO as an oncogenic superenhancer

“…To date, the utility of SEs in targeting strategies for human tumor therapy has been poorly studied [29] . CENPO has been reported to be elevated in various human tumors, including gastric cancer, hepatocellular carcinoma, bladder cancer [ 16 , 30 , 31 ], and is positively associated with poor prognosis, suggesting that CENPO may affect the response to chemotherapy and immunotherapy. However, the prognostic impact of CENPO on LUAD is still unclear.…”

High expression levels of centromere protein O participates in cell activity of human ovarian cancer

“…It has been reported that the expression of CENP-O is low in normal tissues, but high in cancer tissues (He et al, 2022;Wang et al, 2023). To evaluate the signi cance of CENP-O in ovarian cancer, we rst used qPCR to examine the expression levels of CENP-O across ve different ovarian cancer cell lines against normal ovarian cell lines IOSE80.…”